Differential expression of three sucrose-phosphate synthase isoforms during sucrose accumulation in citrus fruits (Citrus unshiu Marc.)

Sucrose accumulation rates, sucrose-phosphate synthase (SPS, EC 2.4.1.14) and soluble sucrose synthase (SuSy, EC 2.4.1.13) activities were measured in internodal tissue from a sugarcane (Saccharum species hybrids) variety N19. The sucrose accumulation rate sharply increases between internodes 3 to 11. In the older internodes SPS activity was at least three times higher than the SuSy activity. A highly significant positive correlation was found between SPS activity and sucrose content. In contrast, no significant correlation was observed between SuSy and sucrose content. In agreement, when radiolabelled glucose was fed to internodes with a high sucrose accumulation rate, label was equally distributed in the hexose moieties of sucrose. This clearly indicates that SPS is the major sucrose synthesis activity in the culm of sugarcane. Different kinetic forms of SPS apparently exist in the internodal tissue at different stages of development.

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Salt-induced Sucrose Accumulation is Mediated by Sucrose-phosphate-synthase in Cyanobacteria

Journal of Plant Physiology ◽

10.1016/s0176-1617(99)80126-6 ◽

1999 ◽

Vol 155 (3) ◽

pp. 424-430 ◽

Cited By ~ 32

Author(s):

Martin Hagemann ◽

Kay Marin

Keyword(s):

Sucrose Phosphate Synthase ◽

Sucrose Accumulation ◽

Sucrose Phosphate ◽

Phosphate Synthase

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Sucrose Accumulation in the Sugarcane Stem Is Regulated by the Difference between the Activities of Soluble Acid Invertase and Sucrose Phosphate Synthase

PLANT PHYSIOLOGY ◽

10.1104/pp.115.2.609 ◽

1997 ◽

Vol 115 (2) ◽

pp. 609-616 ◽

Cited By ~ 108

Author(s):

Y. J. Zhu ◽

E. Komor ◽

P. H. Moore

Keyword(s):

Sucrose Phosphate Synthase ◽

Acid Invertase ◽

Sucrose Accumulation ◽

Soluble Acid Invertase ◽

Soluble Acid ◽

The Difference ◽

Sugarcane Stem ◽

Sucrose Phosphate ◽

Phosphate Synthase

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Cloning and Characterization of a Sucrose Phosphate Synthase-encoding Gene from Muskmelon

Journal of the American Society for Horticultural Science ◽

10.21273/jashs.132.4.557 ◽

2007 ◽

Vol 132 (4) ◽

pp. 557-562 ◽

Cited By ~ 5

Author(s):

Xiyan Yu ◽

Xiufeng Wang ◽

Jide Fan ◽

Hongmei Tian ◽

Chengchao Zheng

Keyword(s):

Critical Role ◽

Sucrose Phosphate Synthase ◽

Transcript Level ◽

Sucrose Accumulation ◽

Mature Fruit ◽

Mrna Accumulation ◽

Reading Frame ◽

Rapid Amplification ◽

Sucrose Phosphate ◽

Phosphate Synthase

Sucrose phosphate synthase [SPS (EC 2.4.1.14)] is thought to play a critical role in sucrose accumulation in muskmelon (Cucumis melo L.) fruit. A full-length cDNA clone encoding sucrose phosphate synthase was isolated from muskmelon by reverse transcriptase–polymerase chain reaction and rapid amplification of cDNA ends. The clone, designated CmSPS1, contains 3377 nucleotides with an open reading frame of 3162 nucleotides. The deduced 1054 amino acids sequence showed high identities with other plant sucrose phosphate synthases. Northern blot analysis indicated that CmSPS1 was expressed in leaves, stems, and mature fruit, but was not detected in roots or flowers. Moreover, the mRNA accumulation of CmSPS1 started at 25 days after pollination (DAP) and reached highest level in mature fruit. Interestingly, both sucrose content and SPS activity increased dramatically between 20 and 30 DAP during fruit development, suggesting that sucrose accumulation may be linked to the CmSPS1 transcript level in muskmelon fruit.

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