Effect of Low Nitrate Supply on Nitrogen Fixation in Alfalfa Root Nodules Induced by Rhizobium meliloti Strains with Varied Nitrate Reductase Activity

1989 ◽  
Vol 135 (2) ◽  
pp. 207-211 ◽  
Author(s):  
Cesáreo Arrese-Igor ◽  
José M. Estavillo ◽  
José I. Peña ◽  
Carmen Gonzalez-Murua ◽  
Pedro M. Aparicio-Tejo
Keyword(s):  
Nitrogen Fixation ◽  
Nitrate Reductase ◽  
Nitrate Reductase Activity ◽  
Rhizobium Meliloti ◽  
Reductase Activity ◽  
Root Nodules ◽  
Nitrate Supply ◽  
Alfalfa Root

Phenotypic reversion of nitrogenase in pleiotropic mutants of Rhizobium meliloti

1979 ◽  
Vol 25 (3) ◽  
pp. 298-301 ◽  
Author(s):  
Ilona Barabás ◽  
Tibor Sik
Keyword(s):  
Amino Acids ◽  
Nitrogen Fixation ◽  
Nitrate Reductase ◽  
Nitrate Reductase Activity ◽  
Symbiotic Nitrogen Fixation ◽  
Rhizobium Meliloti ◽  
Nitrogenase Activity ◽  
Reductase Activity ◽  
Amino Acid Utilization ◽  
Phenotypic Reversion

In two out of three pleiotropic mutants of Rhizobium meliloti, defective in nitrate reductase induced by amino acid utilization in vegetative bacteria and in symbiotic nitrogen fixation, nitrogenase activity could be restored completely by purines and partially by the amino acids L-glutamate, L-aspartate, L-glutamine, and L-asparagine. The compounds restoring effectiveness in nitrogen fixation did not restore nitrate reductase activity in vegetative bacteria. The restoration of effectiveness supports our earlier conclusion that the mutation is not in the structural gene for a suggested common subunit of nitrogenase and nitrate reductase.

Nitrate reductase activities of rhizobia and the correlation between nitrate reduction and nitrogen fixation

1979 ◽  
Vol 25 (10) ◽  
pp. 1169-1174 ◽  
Author(s):  
James R. Manhart ◽  
Peter P. Wong
Keyword(s):  
Nitrogen Fixation ◽  
Nitrate Reductase ◽  
Nitrate Reduction ◽  
Nitrate Reductase Activity ◽  
Reductase Activity ◽  
Root Nodules ◽  
Sole Source ◽  
Rhizobium Japonicum ◽  
Rhizobium Trifolii ◽  
Rhizobium Species

All species of Rhizobium except R. lupini had nitrate reductase activity. Only R. lupini was incapable of growth with nitrate as the sole source of nitrogen. However, the conditions necessary for the induction of nitrate reductase varied among species of Rhizobium. Rhizobium japonicum and some Rhizobium species of the cowpea strains expressed nitrate reductase activities both in the root nodules of appropriate leguminous hosts and when grown in the presence of nitrate. Rhizobium trifolii, R. phaseoli, and R. legnminosarum did not express nitrate reductase activities in the root nodules, but they did express them when grown in the presence of nitrate. In bacteroids of R. japonicum and some strains of cowpea Rhizobium, high N2 fixation activities were accompanied by high nitrate reductase activities. In bacteroids of R. trifolii, R. leguminosarum, and R. phaseoli, high N2 fixation activities were not accompanied by high nitrate reductase activities.

Nitrate modification of photosynthesis and photoassimilate export in young nodulated soybean plants

1982 ◽  
Vol 60 (12) ◽  
pp. 2665-2670 ◽  
Author(s):  
D. J. Ursino ◽  
D. M. Hunter ◽  
R. D. Laing ◽  
J. L. S. Keighley
Keyword(s):  
Nitrogen Fixation ◽  
Nitrate Reductase ◽  
Nutrient Solution ◽  
Nitrate Reductase Activity ◽  
Reductase Activity ◽  
Nodule Development ◽  
Nitrate Availability ◽  
Data Support ◽  
Nutrient Fertilization ◽  
Soybean Plants

Soybean plants (cv. Harosoy 63) inoculated with rhizobia were germinated from seed and beginning on day 7 after planting were subjected to one of four patterns of nutrient fertilization. One group received a nutrient solution containing 5 mM nitrate, a second group received nitrate-free nutrient solution, and two other groups received nitrate-containing solution either from days 7 to 13 or from days 14 to 20. On day 21 rates of leaf photosynthetic CO2 uptake and nitrate reductase activity were measured, as well as the capacities of the leaf to export recent photosynthate and of the nodules to reduce acetylene. The data support the hypothesis that sufficient nitrate availability in the leaves of young soybean plants can modify both photosynthetic CO2 uptake and light-mediated photoassimilate export to an extent that nodule development and the capacity for nitrogen fixation are reduced.

EFFECTS OF NITRATE SUPPLY ON PLANT GROWTH, NITROGEN, PHOSPHORUS AND POTASSIUM ACCUMULATION, AND NITRATE REDUCTASE ACTIVITY IN CRAMBE

2013 ◽  
Vol 36 (2) ◽  
pp. 275-283 ◽  
Author(s):  
Diego de Mello Conde de Brito ◽  
Carlos Diego dos Santos ◽  
Fabíola Vieira Gonçalves ◽  
Rosane Nora Castro ◽  
Sonia Regina de Souza
Keyword(s):  
Nitrate Reductase ◽  
Plant Growth ◽  
Nitrate Reductase Activity ◽  
Reductase Activity ◽  
Potassium Accumulation ◽  
Nitrate Supply ◽  
Phosphorus And Potassium ◽  
Nitrogen Phosphorus

Further Studies on the Induction of Nitrate Reductase by Arginine in the Filamentous Cyanobacterium Oscillatoria chalybea

1992 ◽  
Vol 47 (7-8) ◽  
pp. 540-544 ◽  
Author(s):  
J. Bednarz ◽  
G. H. Schmid
Keyword(s):  
Nitrogen Fixation ◽  
Nitrate Reductase ◽  
Nitrate Reductase Activity ◽  
Reductase Activity ◽  
Filamentous Cyanobacterium ◽  
Nitrogen Fixation Activity ◽  
Fixation Activity ◽  
Cyanobacterium Anabaena ◽  
Oscillatoria Chalybea ◽  
Anabaena Pcc7120

In an earlier publication we reported on the role of arginine for the development of nitrate reductase activity in cells of the filamentous cyanobacterium Oscillatoria chalybea (Bednarz and Schmid, Z. Naturforsch. 46c, 591 (1991)). In the present paper we present further evidence that arginine is the natural inducer for nitrate reductase activity. Thus, we show that the induction is regulated by transcription, probably related to the apoprotein or the molybdenumcofactor. We also examined the influence of arginine on nitrate reductase activity in the filamentous cyanobacterium Anabaena PCC7120. In contrast to Oscillatoria chalybea this cyanobacterium forms heterocysts and shows nitrogen fixation activity. Like in Oscillatoria chalybea nitrate reductase activity in Anabaena PCC7120 is stimulated in the presence of arginine as the sole nitrogen source. However, this stimulation is limited to an early growth stage. Subsequently, nitrogen fixation activity appears and nitrate reductase activity decreases.

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