High-level and stable accumulation of single-chain Fv antibodies in plant storage organs

1998 ◽  
Vol 152 (6) ◽  
pp. 708-711 ◽  
Author(s):  
Udo Conrad ◽  
Ulrike Fiedler ◽  
Olga Artsaenko ◽  
Julian Phillips
Keyword(s):  
Single Chain ◽  
Single Chain Fv ◽  
Storage Organs ◽  
High Level ◽  
Plant Storage

scholarly journals High-Level Expression of Single-Chain Fv-Fc Fusion Protein in Serum and Egg White of Genetically Manipulated Chickens by Using a Retroviral Vector

2005 ◽  
Vol 79 (17) ◽  
pp. 10864-10874 ◽  
Author(s):  
Masamichi Kamihira ◽  
Ken-ichiro Ono ◽  
Kazuhisa Esaka ◽  
Ken-ichi Nishijima ◽  
Ryoko Kigaku ◽  
...  
Keyword(s):  
Transgene Expression ◽  
Leukemia Virus ◽  
Retroviral Vector ◽  
Single Chain ◽  
Transgenic Chicken ◽  
Chicken Embryos ◽  
Single Chain Fv ◽  
High Level ◽  
Transgenic Chickens ◽  
Transgenic Progeny

ABSTRACT We report here the generation of transgenic chickens using a retroviral vector for the production of recombinant proteins. It was found that the transgene expression was suppressed when a Moloney murine leukemia virus-based retroviral vector was injected into chicken embryos at the blastodermal stage. When a concentrated viral solution was injected into the heart of developing embryos after 50 to 60 h of incubation, transgene expression was observed throughout the embryo, including the gonads. For practical production, a retroviral vector encoding an expression cassette of antiprion single-chain Fv fused with the Fc region of human immunoglobulin G1 (scFv-Fc) was injected into chicken embryos. The birds that hatched stably produced scFv-Fc in their serum and eggs at high levels (∼5.6 mg/ml). We obtained transgenic progeny from a transgenic chicken generated with this procedure. The transgene was stably integrated into the chromosomes of transgenic progeny. The transgenic progeny also expressed scFv-Fc in the serum and eggs.

An optimized fermentation process for high-level production of a single-chain Fv antibody fragment in Pichia pastoris

2004 ◽  
Vol 37 (1) ◽  
pp. 18-26 ◽  
Author(s):  
Leonardo M Damasceno ◽  
Itzcoatl Pla ◽  
Hyun-Joo Chang ◽  
Leonard Cohen ◽  
Gerd Ritter ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Fermentation Process ◽  
Antibody Fragment ◽  
Single Chain ◽  
Single Chain Fv ◽  
Fv Antibody ◽  
High Level ◽  
Level Production ◽  
Single Chain Fv Antibody

High-level secretion of two antibody single chain Fv fragments by Pichia pastoris

1997 ◽  
Vol 201 (1) ◽  
pp. 67-75 ◽  
Author(s):  
Patrick Eldin ◽  
Mary E Pauza ◽  
Yoko Hieda ◽  
Gaofeng Lin ◽  
Michael P Murtaugh ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Single Chain ◽  
Single Chain Fv ◽  
High Level

High-level expression in insect cells and purification of secreted monomeric single-chain Fv antibodies

1996 ◽  
Vol 195 (1-2) ◽  
pp. 93-101 ◽  
Author(s):  
Titus Kretzschmar ◽  
Laurent Aoustin ◽  
Otto Zingel ◽  
Marcello Marangi ◽  
Bénédicte Vonach ◽  
...  
Keyword(s):  
Insect Cells ◽  
Single Chain ◽  
High Level Expression ◽  
Single Chain Fv ◽  
High Level

scholarly journals High-level secretion of recombinant monomeric murine and human single-chain Fv antibodies from Drosophila S2 cells

2011 ◽  
Vol 25 (2) ◽  
pp. 59-66 ◽  
Author(s):  
Allissia A. Gilmartin ◽  
Benjamin Lamp ◽  
Till Rümenapf ◽  
Mats A.A. Persson ◽  
Félix A. Rey ◽  
...  
Keyword(s):  
S2 Cells ◽  
Single Chain ◽  
Single Chain Fv ◽  
Drosophila S2 Cells ◽  
High Level

Metabolic signalling and the partitioning of resources in plant storage organs

1999 ◽  
Vol 133 (3) ◽  
pp. 243-249 ◽  
Author(s):  
NIGEL G. HALFORD
Keyword(s):  
Dry Weight ◽  
Wild Relatives ◽  
Crop Plants ◽  
Wild Plants ◽  
Young Plant ◽  
Carbon And Nitrogen ◽  
Cultivated Potato ◽  
Metabolic Signalling ◽  
Storage Organs ◽  
Plant Storage

The most important harvested organs of crop plants, such as seeds, tubers and fruits, are often described as assimilate sinks. They play little or no part in the fixation of carbon through the production of sugars through photosynthesis, or in the uptake of nitrogen and sulphur, but import these assimilated resources to support metabolism and to store them in the form of starch, oils and proteins. Wild plants store resources in seeds and tubers to later support an emergent young plant. Cultivated crops are effectively storing resources to provide us with food and many have been bred to accumulate much more than would be required otherwise. For example, approximately 80% of a cultivated potato plant's dry weight is contained in its tubers, ten times the proportion in the tubers of its wild relatives (Inoue & Tanaka 1978). Cultivation and breeding has brought about a shift in the partitioning of carbon and nitrogen assimilate between the organs of the plant.

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