Duck hepatitis B virus: Cloning and subcloning of the viral genome

ABSTRACT Hepatitis B virus assembly begins with the packaging of an RNA pregenome into intracellular nucleocapsids, with subsequent reverse transcription within these nucleocapsids converting the RNA into a characteristic, partially double-stranded DNA, which, alone, is found in enveloped extracellular virions as the viral genome. Using a synchronized replication system for the duck hepatitis B virus, together with a stringent two-step assay for virion secretion, we demonstrate that this selective genome secretion results from an intrinsic secretion competence gained only by the nucleocapsids at the late stage of reverse transcription.

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Faculty Opinions recommendation of Translation of duck hepatitis B virus reverse transcriptase by ribosomal shunting.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1021978.247821 ◽

2004 ◽

Author(s):

Don Coen

Keyword(s):

Hepatitis B Virus ◽

Hepatitis B ◽

Reverse Transcriptase ◽

Duck Hepatitis B Virus ◽

Duck Hepatitis ◽

B Virus

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Integrated structures of duck hepatitis B virus DNA in hepatocellular carcinoma.

Journal of Virology ◽

10.1128/jvi.62.3.861-865.1988 ◽

1988 ◽

Vol 62 (3) ◽

pp. 861-865 ◽

Cited By ~ 13

Author(s):

F Imazeki ◽

K Yaginuma ◽

M Omata ◽

K Okuda ◽

M Kobayashi ◽

...

Keyword(s):

Hepatocellular Carcinoma ◽

Hepatitis B Virus ◽

Hepatitis B ◽

Duck Hepatitis B Virus ◽

Hepatitis B Virus Dna ◽

Duck Hepatitis ◽

B Virus ◽

Integrated Structures

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Fine Mapping of Neutralization Epitopes on Duck Hepatitis B Virus (DHBV) pre-S Protein Using Monoclonal Antibodies and Overlapping Peptides

Virology ◽

10.1006/viro.1993.1024 ◽

1993 ◽

Vol 192 (1) ◽

pp. 217-223 ◽

Cited By ~ 23

Author(s):

Sylvie Chassot ◽

Véronique Lambert ◽

Alan Kay ◽

Catherine Godinot ◽

Bernard Roux ◽

...

Keyword(s):

Monoclonal Antibodies ◽

Hepatitis B Virus ◽

Hepatitis B ◽

Fine Mapping ◽

Duck Hepatitis B Virus ◽

S Protein ◽

Duck Hepatitis ◽

B Virus

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Duck hepatitis B virus: a model to assess efficacy of disinfectants against hepadnavirus infectivity

Epidemiology and Infection ◽

10.1017/s0950268800067480 ◽

1991 ◽

Vol 106 (3) ◽

pp. 435-443 ◽

Cited By ~ 33

Author(s):

S. M. Murray ◽

J. S. Freiman ◽

K. Vickery ◽

D. Lim ◽

Y. E. Cossart ◽

...

Keyword(s):

Hepatitis B Virus ◽

Hepatitis B ◽

Room Temperature ◽

Virus Titre ◽

Experimental Protocol ◽

Duck Hepatitis B Virus ◽

Duck Hepatitis ◽

B Virus ◽

Undiluted Serum ◽

Sterile Filter

SUMMARYThe efficacy of three proprietary glutaraldehyde disinfectants and their component bases was assessed using the duck hepatitis B virus (DHBV) model. Inactivation of infectivity of undiluted serum containing 106·8ID50/ml DHBV was assessed after a mixture with an equal volume of disinfectant had stood at room temperature for 10 min. A dried spill of infectious serum was simulated using sterile filter paper disks, saturated with serum containing DHBV, dried and then exposed to test disinfectant for 10 min. Residual infectivity, and hence the reduction in virus titre, was determined by inoculation of dilutions of the treated samples into 1-day-old ducklings. A greater than 3 log10reduction in virus titre could be demonstrated for the disinfectants as well as for some of their component bases. Disinfectant activity varied according to the method of viral presentation but a reduction of exposure time from 10 to 2·5 min did not diminish activity. The experimental protocol permits a comparative and quantitative assessment of the efficacy of both established and new disinfectants.

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