A truncated variant of the hepatitis C virus core induces a slow but potent immune response in mice following DNA immunization

Vaccine ◽  
2000 ◽  
Vol 19 (7-8) ◽  
pp. 992-997 ◽  
Author(s):  
Santiago Dueñas-Carrera ◽  
Liz Alvarez-Lajonchere ◽  
Julio C. Alvarez-Obregón ◽  
Antonieta Herrera ◽  
Lázaro J. Lorenzo ◽  
...  
Keyword(s):  
Immune Response ◽  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Dna Immunization ◽  
Virus Core ◽  
Potent Immune Response

scholarly journals Additives and Protein-DNA Combinations Modulate the Humoral Immune Response Elicited by a Hepatitis C Virus Core-encoding Plasmid in Mice

2002 ◽  
Vol 97 (1) ◽  
pp. 95-99
Author(s):  
Liz Alvarez-Lajonchere ◽  
Santiago Dueñas-Carrera ◽  
Ariel Viña ◽  
Thelvia Ramos ◽  
Dagmara Pichardo ◽  
...  
Keyword(s):  
Immune Response ◽  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Humoral Immune Response ◽  
Humoral Immune ◽  
Virus Core

DNA immunization with a plasmid carrying the gene of hepatitis C virus protein 5A (NS5A) induces an effective cellular immune response

2010 ◽  
Vol 44 (2) ◽  
pp. 245-253 ◽  
Author(s):  
O. V. Masalova ◽  
E. I. Lesnova ◽  
V. V. Grabovetskii ◽  
O. A. Smirnova ◽  
T. I. Ulanova ◽  
...  
Keyword(s):  
Immune Response ◽  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Cellular Immune Response ◽  
Virus Protein ◽  
Dna Immunization ◽  
Cellular Immune

scholarly journals Genetic Immunization of Wild-Type and Hepatitis C Virus Transgenic Mice Reveals a Hierarchy of Cellular Immune Response and Tolerance Induction against Hepatitis C Virus Structural Proteins

2001 ◽  
Vol 75 (24) ◽  
pp. 12121-12127 ◽  
Author(s):  
Jujin Satoi ◽  
Kazumoto Murata ◽  
Martin Lechmann ◽  
Elanchezhiyan Manickan ◽  
Zhensheng Zhang ◽  
...  
Keyword(s):  
Immune Response ◽  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Transgenic Mice ◽  
Structural Proteins ◽  
Dna Immunization ◽  
Wild Type ◽  
Genetic Immunization ◽  
Cellular Immune ◽  
Hcv Structural Proteins

ABSTRACT To study the effect of genetic immunization on transgenic expression of hepatitis C virus (HCV) proteins, we evaluated the immunological response of HCV transgenic mice to HCV expression plasmids. FVB/n transgenic mice expressing HCV structural proteins (core, E1, and E2) and wild-type (WT) FVB/n mice were immunized intramuscularly with plasmids expressing core (pHCVcore) or core/E1/E2 (pHCVSt). After immunization, HCV-specific humoral and cellular immune response was studied. Both WT and transgenic mice immunized with either HCV construct produced antibodies and exhibited T-cell proliferative responses against core or envelope. In WT mice immunized with pHCVSt, cytotoxic T-lymphocyte (CTL) activities were detected against E2 but not against core or E1, whereas strong CTL activities against core could be detected in WT mice immunized with pHCVcore. In pHCVSt-immunized, transgenic mice, CTL activities against the core or envelope were completely absent, but core-specific CTL activities could be detected in pHCVcore-immunized transgenic mice. A similar pattern of immune responses was also observed in other mouse strains, including a transgenic line expressing human HLA-A2.1 molecules (AAD mice). Despite the presence of a peripheral cellular immunity against HCV, no liver pathology or lymphocytic infiltrate was observed in these transgenic mice. Our study suggests a hierarchy of CTL response against the HCV structural proteins (E2 > core > E1) in vivo when the proteins are expressed as a polyprotein. The HCV transgenic mice can be induced by DNA immunization to generate anti-HCV antibodies and anticore CTLs. However, they are tolerant at the CTL level against the E2 protein despite DNA immunization.

scholarly journals The Role of Micronutrients in the Infection and Subsequent Response to Hepatitis C Virus

Cells ◽  
2019 ◽  
Vol 8 (6) ◽  
pp. 603 ◽  
Author(s):  
Sunil Gupta ◽  
Scott A. Read ◽  
Nicholas A. Shackel ◽  
Lionel Hebbard ◽  
Jacob George ◽  
...  
Keyword(s):  
Immune Response ◽  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Vitamin A ◽  
Innate Immune ◽  
Micronutrient Deficiencies ◽  
Strong Immune Response ◽  
Subsequent Response ◽  
Potent Immune Response

Micronutrient deficiencies develop for a variety of reasons, whether geographic, socioeconomic, nutritional, or as a result of disease pathologies such as chronic viral infection. As micronutrients are essential for a strong immune response, deficiencies can significantly dampen both the innate and the adaptive arms of antiviral immunity. The innate immune response in particular is crucial to protect against hepatitis C virus (HCV), a hepatotropic virus that maintains chronic infection in up to 80% of individuals if left untreated. While many micronutrients are required for HCV replication, an overlapping group of micronutrients are also necessary to enact a potent immune response. As the liver is responsible for the storage and metabolism of many micronutrients, HCV persistence can influence the micronutrients’ steady state to benefit viral persistence both directly and by weakening the antiviral response. This review will focus on common micronutrients such as zinc, iron, copper, selenium, vitamin A, vitamin B12, vitamin D and vitamin E. We will explore their role in the pathogenesis of HCV infection and in the response to antiviral therapy. While chronic hepatitis C virus infection drives deficiencies in micronutrients such as zinc, selenium, vitamin A and B12, it also stimulates copper and iron excess; these micronutrients influence antioxidant, inflammatory and immune responses to HCV.

scholarly journals Expression and immune response to hepatitis C virus core DNA-based vaccine constructs

Hepatology ◽  
1996 ◽  
Vol 24 (1) ◽  
pp. 14-20 ◽  
Author(s):  
K Tokushige ◽  
T Wakita ◽  
C Pachuk ◽  
D Moradpour ◽  
D B Weiner ◽  
...  
Keyword(s):  
Immune Response ◽  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Virus Core

Repeated administration of hepatitis C virus core-encoding plasmid to mice does not necessarily increase the immune response generated against this antigen

2001 ◽  
Vol 33 (1) ◽  
pp. 47 ◽  
Author(s):  
Santiago Dueñas-Carrera ◽  
Liz Alvarez-Lajonchere ◽  
Julio Cesar Alvarez ◽  
Thelvia Ramos ◽  
Dagmara Pichardo ◽  
...  
Keyword(s):  
Immune Response ◽  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Repeated Administration ◽  
Virus Core
Sign in / Sign up

Export Citation Format

Share Document