genetic immunization
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2021 ◽  
Vol 155 ◽  
pp. 104888
Author(s):  
Narjes Yektaeian ◽  
Abdorrasoul Malekpour ◽  
Amir Atapour ◽  
Tahereh Davoodi ◽  
Gholamreza Hatam

2020 ◽  
Vol 140 (12) ◽  
pp. 2528-2531.e2 ◽  
Author(s):  
Geza Erdos ◽  
Stephen C. Balmert ◽  
Cara Donahue Carey ◽  
Gabriel D. Falo ◽  
Nikita A. Patel ◽  
...  

2020 ◽  
Vol 21 (19) ◽  
pp. 7074
Author(s):  
Julie Pelletier ◽  
Hervé Agonsanou ◽  
Fabiana Manica ◽  
Elise G. Lavoie ◽  
Mabrouka Salem ◽  
...  

We have generated polyclonal and monoclonal antibodies by genetic immunization over the last two decades. In this paper, we present our most successful methodology acquired over these years and present the animals in which we obtained the highest rates of success. The technique presented is convenient, easy, affordable, and generates antibodies against mammalian proteins in their native form. This protocol requires neither expensive equipment, such as a gene gun, nor sophisticated techniques such as the conjugation of gold microspheres, electroporation, or surgery to inject in lymph nodes. The protocol presented uses simply the purified plasmid expressing the protein of interest under a strong promoter, which is injected at intramuscular and intradermal sites. This technique was tested in five species. Guinea pigs were the animals of choice for the production of polyclonal antibodies. Monoclonal antibodies could be generated in mice by giving, as a last injection, a suspension of transfected cells. The antibodies detected their antigens in their native forms. They were highly specific with very low non-specific background levels, as assessed by immune-blots, immunocytochemistry, immunohistochemistry and flow cytometry. We present herein a detailed and simple procedure to successfully raise specific antibodies against native proteins.


2020 ◽  
Vol 110 ◽  
pp. 254-265
Author(s):  
Leonardo Rojas-Sánchez ◽  
Ejuan Zhang ◽  
Viktoriya Sokolova ◽  
Maohua Zhong ◽  
Hu Yan ◽  
...  

2019 ◽  
Vol 10 (2) ◽  
Author(s):  
Agathe Dubuisson ◽  
Cécile Favreau ◽  
Eric Fourmaux ◽  
Sabrina Lareure ◽  
Rafael Rodrigues-Saraiva ◽  
...  
Keyword(s):  

Vaccine ◽  
2018 ◽  
Vol 36 (22) ◽  
pp. 3072-3078 ◽  
Author(s):  
D.V. Lopez-Guerrero ◽  
N. Arias ◽  
L. Gutierrez-Xicotencatl ◽  
L. Chihu-Amparan ◽  
A. González ◽  
...  

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