Differences in sulfation patterns of heparan sulfate derived from human bone marrow and umbilical vein endothelial cells

2001 ◽  
Vol 29 (7) ◽  
pp. 884-893 ◽  
Author(s):  
T Netelenbos
Keyword(s):  
Bone Marrow ◽  
Endothelial Cells ◽  
Heparan Sulfate ◽  
Umbilical Vein ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Umbilical Vein Endothelial Cells

scholarly journals Chemotactic and Angiogenic Potential of Mineralized Collagen Scaffolds Functionalized with Naturally Occurring Bioactive Factor Mixtures to Stimulate Bone Regeneration

2021 ◽  
Vol 22 (11) ◽  
pp. 5836
Author(s):  
Henriette Bretschneider ◽  
Mandy Quade ◽  
Anja Lode ◽  
Michael Gelinsky ◽  
Stefan Rammelt ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Platelet Rich Plasma ◽  
Umbilical Vein ◽  
Release Kinetics ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Collagen Scaffolds ◽  
Angiogenic Potential ◽  
Naturally Occurring ◽  
Mineralized Collagen

To develop cost-effective and efficient bone substitutes for improved regeneration of bone defects, heparin-modified mineralized collagen scaffolds were functionalized with concentrated, naturally occurring bioactive factor mixtures derived from adipose tissue, platelet-rich plasma and conditioned medium from a hypoxia-treated human bone marrow-derived mesenchymal stem cell line. Besides the analysis of the release kinetics of functionalized scaffolds, the bioactivity of the released bioactive factors was tested with regard to chemotaxis and angiogenic tube formation. Additionally, functionalized scaffolds were seeded with human bone marrow-derived mesenchymal stromal cells (hBM-MSC) and their osteogenic and angiogenic potential was investigated. The release of bioactive factors from the scaffolds was highest within the first 3 days. Bioactivity of the released factors could be confirmed for all bioactive factor mixtures by successful chemoattraction of hBM-MSC in a transwell assay as well as by the formation of prevascular structures in a 2D co-culture system of hBM-MSC and human umbilical vein endothelial cells. The cells seeded directly onto the functionalized scaffolds were able to express osteogenic markers and form tubular networks. In conclusion, heparin-modified mineralized collagen scaffolds could be successfully functionalized with naturally occurring bioactive factor mixtures promoting cell migration and vascularization.

Immortalisation of human bone marrow endothelial cells: characterisation of new cell lines

2000 ◽  
Vol 30 (7) ◽  
pp. 618-629 ◽  
Author(s):  
Rood ◽  
Calafat ◽  
Kr. Von dem Borne ◽  
Gerritsen ◽  
Van Der Schoot
Keyword(s):  
Bone Marrow ◽  
Endothelial Cells ◽  
Cell Lines ◽  
Human Bone ◽  
Human Bone Marrow
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