Expression analysis of the 5′-upstream region of mouse P/Q-type Ca2+ channel α lA subunit gene fused to Escherichia coli lacZ reporter gene in the spinal cord using transgenic mice

2000 ◽  
Vol 284 (1-2) ◽  
pp. 9-12 ◽  
Author(s):  
Eiki Takahashi ◽  
Noriko Kajiwara ◽  
Keiko Furuya ◽  
Fumihiro Sugiyama ◽  
Ken-ichi Yagami
Keyword(s):  
Escherichia Coli ◽  
Spinal Cord ◽  
Transgenic Mice ◽  
Reporter Gene ◽  
Expression Analysis ◽  
Upstream Region ◽  
Subunit Gene ◽  
Lacz Reporter ◽  
Lacz Reporter Gene

Expression analysis of Escherichia coli lacZ reporter gene in transgenic mice

2000 ◽  
Vol 5 (2) ◽  
pp. 159-166 ◽  
Author(s):  
Eiki Takahashi ◽  
Norimasa Miyamoto ◽  
Noriko Kajiwara ◽  
Keiko Furuya ◽  
Keiko Yanai-Taniguchi ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Transgenic Mice ◽  
Reporter Gene ◽  
Expression Analysis ◽  
Lacz Reporter ◽  
Lacz Reporter Gene

scholarly journals Analysis of the 5'-upstream region of mouse P/Q-type Ca2+ channel alpha1A subunit gene for expression in pancreatic islet beta cells using transgenic mice and HIT-T15 cells

2000 ◽  
Vol 24 (2) ◽  
pp. 225-232 ◽  
Author(s):  
E Takahashi ◽  
N Miyamoto ◽  
T Nagasu
Keyword(s):  
Gene Expression ◽  
Transgenic Mice ◽  
Beta Cell ◽  
Reporter Gene ◽  
Promoter Activity ◽  
Upstream Region ◽  
Subunit Gene ◽  
Reporter Expression ◽  
Hit Cells ◽  
Cell Expression

The omega-agatoxin-IVA-sensitive P/Q-type Ca(2+) channel plays a role in insulin release from the pancreatic islets of beta cells. To dissect the molecular mechanisms underlying beta cell expression of the P/Q-type channel, we characterized the 5'-upstream region of the mouse alpha(1A) subunit gene using transgenic mice and HIT insulinoma cells. The E. coli lacZ reporter gene was expressed in pancreatic acini and islets in transgenic mice carrying the 6.3 kb or 3.0 kb of the 5'-upstream region, although those with 1.5 kb or 0. 5 kb of the 5'-upstream region failed to show reporter expression on histological examination. As the expression of alpha(1A)subunit gene could not be detected in acini using RT-PCR analysis, the reporter expression in acini might have been ectopic expression. When linked to the placental alkaline phosphatase reporter gene to examine promoter activity for beta cell expression, the 6.3 kb and 3.0 kb fragment of the 5'-upstream region, but not the smaller 1.5 kb fragment, were able to drive reporter gene expression in HIT cells. The sequence between 3.0 and 1.5 kb upstream of the start codon enhanced thymidine kinase promoter activity in HIT cells, but not in fibroblast NIH3T3 cells. These results suggested that the beta cell-specific elements of the alpha(1A) subunit gene are likely to be located in the distal upstream region (-3021 to-1563) of the 5'-upstream sequence and that the 6.3 kb fragment of the 5'-upstream region alone might be a lack of a negative cis-regulatory element(s) to suppress the alpha(1A) subunit gene expression in acini.

Mosaic expression of LacZ reporter gene controlled by 5′-regulatory sequences of alpha-S 1 -Casein gene in transgenic mice

2009 ◽  
Vol 3 (5) ◽  
pp. 409-416
Author(s):  
I. A. Serova ◽  
L. E. Andreeva ◽  
N. V. Khaidarova ◽  
L. P. B. Dias ◽  
G. A. Dvoryanchikov ◽  
...  
Keyword(s):  
Transgenic Mice ◽  
Reporter Gene ◽  
Regulatory Sequences ◽  
Lacz Reporter ◽  
Lacz Reporter Gene ◽  
Casein Gene ◽  
Mosaic Expression

Expression pattern of lacZ reporter gene representing connexin36 in transgenic mice

2004 ◽  
Vol 473 (4) ◽  
pp. 511-525 ◽  
Author(s):  
Joachim Degen ◽  
Carola Meier ◽  
Ruben S. Van Der Giessen ◽  
Goran Söhl ◽  
Elisabeth Petrasch-Parwez ◽  
...  
Keyword(s):  
Transgenic Mice ◽  
Expression Pattern ◽  
Reporter Gene ◽  
Lacz Reporter ◽  
Lacz Reporter Gene

Promoter Assessment in Hansenula polymorpha Using a lacZ Reporter Gene

2003 ◽  
pp. 101-115
Author(s):  
Manfred Suckow ◽  
Martina Kutzner ◽  
Carsten Amuel ◽  
Cornelis P. Hollenberg ◽  
Gerd Gellissen
Keyword(s):  
Reporter Gene ◽  
Hansenula Polymorpha ◽  
Lacz Reporter ◽  
Lacz Reporter Gene
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