scholarly journals Neuron-specific expression of reporter gene in transgenic mice carrying the 5′-upstream region of mouse P/Q-type Ca2+ channel α1A subunit gene fused to E. coli lacZ reporter gene

1999 ◽  
Vol 850 (1-2) ◽  
pp. 47-54 ◽  
Author(s):  
Eiki Takahashi ◽  
Norimasa Miyamoto ◽  
Tohru Oki ◽  
Noriko Kajiwara ◽  
Keiko Furuya ◽  
...  
Keyword(s):  
Transgenic Mice ◽  
Reporter Gene ◽  
Upstream Region ◽  
Subunit Gene ◽  
Lacz Reporter ◽  
Lacz Reporter Gene ◽  
Specific Expression ◽  
E Coli

scholarly journals Analysis of the 5'-upstream region of mouse P/Q-type Ca2+ channel alpha1A subunit gene for expression in pancreatic islet beta cells using transgenic mice and HIT-T15 cells

2000 ◽  
Vol 24 (2) ◽  
pp. 225-232 ◽  
Author(s):  
E Takahashi ◽  
N Miyamoto ◽  
T Nagasu
Keyword(s):  
Gene Expression ◽  
Transgenic Mice ◽  
Beta Cell ◽  
Reporter Gene ◽  
Promoter Activity ◽  
Upstream Region ◽  
Subunit Gene ◽  
Reporter Expression ◽  
Hit Cells ◽  
Cell Expression

The omega-agatoxin-IVA-sensitive P/Q-type Ca(2+) channel plays a role in insulin release from the pancreatic islets of beta cells. To dissect the molecular mechanisms underlying beta cell expression of the P/Q-type channel, we characterized the 5'-upstream region of the mouse alpha(1A) subunit gene using transgenic mice and HIT insulinoma cells. The E. coli lacZ reporter gene was expressed in pancreatic acini and islets in transgenic mice carrying the 6.3 kb or 3.0 kb of the 5'-upstream region, although those with 1.5 kb or 0. 5 kb of the 5'-upstream region failed to show reporter expression on histological examination. As the expression of alpha(1A)subunit gene could not be detected in acini using RT-PCR analysis, the reporter expression in acini might have been ectopic expression. When linked to the placental alkaline phosphatase reporter gene to examine promoter activity for beta cell expression, the 6.3 kb and 3.0 kb fragment of the 5'-upstream region, but not the smaller 1.5 kb fragment, were able to drive reporter gene expression in HIT cells. The sequence between 3.0 and 1.5 kb upstream of the start codon enhanced thymidine kinase promoter activity in HIT cells, but not in fibroblast NIH3T3 cells. These results suggested that the beta cell-specific elements of the alpha(1A) subunit gene are likely to be located in the distal upstream region (-3021 to-1563) of the 5'-upstream sequence and that the 6.3 kb fragment of the 5'-upstream region alone might be a lack of a negative cis-regulatory element(s) to suppress the alpha(1A) subunit gene expression in acini.

Mosaic expression of LacZ reporter gene controlled by 5′-regulatory sequences of alpha-S 1 -Casein gene in transgenic mice

2009 ◽  
Vol 3 (5) ◽  
pp. 409-416
Author(s):  
I. A. Serova ◽  
L. E. Andreeva ◽  
N. V. Khaidarova ◽  
L. P. B. Dias ◽  
G. A. Dvoryanchikov ◽  
...  
Keyword(s):  
Transgenic Mice ◽  
Reporter Gene ◽  
Regulatory Sequences ◽  
Lacz Reporter ◽  
Lacz Reporter Gene ◽  
Casein Gene ◽  
Mosaic Expression

Expression analysis of Escherichia coli lacZ reporter gene in transgenic mice

2000 ◽  
Vol 5 (2) ◽  
pp. 159-166 ◽  
Author(s):  
Eiki Takahashi ◽  
Norimasa Miyamoto ◽  
Noriko Kajiwara ◽  
Keiko Furuya ◽  
Keiko Yanai-Taniguchi ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Transgenic Mice ◽  
Reporter Gene ◽  
Expression Analysis ◽  
Lacz Reporter ◽  
Lacz Reporter Gene

Expression pattern of lacZ reporter gene representing connexin36 in transgenic mice

2004 ◽  
Vol 473 (4) ◽  
pp. 511-525 ◽  
Author(s):  
Joachim Degen ◽  
Carola Meier ◽  
Ruben S. Van Der Giessen ◽  
Goran Söhl ◽  
Elisabeth Petrasch-Parwez ◽  
...  
Keyword(s):  
Transgenic Mice ◽  
Expression Pattern ◽  
Reporter Gene ◽  
Lacz Reporter ◽  
Lacz Reporter Gene

scholarly journals Tissue-specific regulation of the mouse Pkhd1 (ARPKD) gene promoter

2014 ◽  
Vol 307 (3) ◽  
pp. F356-F368 ◽  
Author(s):  
Scott S. Williams ◽  
Patricia Cobo-Stark ◽  
Sachin Hajarnis ◽  
Karam Aboudehen ◽  
Xinli Shao ◽  
...  
Keyword(s):  
Binding Site ◽  
Reporter Gene ◽  
Lacz Reporter ◽  
Polycystic Kidney ◽  
Lacz Reporter Gene ◽  
Specific Expression ◽  
Tissue Specific ◽  
Tissue Specific Expression ◽  
Endogenous Expression ◽  
Collecting Ducts

Autosomal recessive polycystic kidney disease, an inherited disorder characterized by the formation of cysts in renal collecting ducts and biliary dysgenesis, is caused by mutations of the polycystic kidney and hepatic disease 1 ( PKHD1) gene. Expression of PKHD1 is tissue specific and developmentally regulated. Here, we show that a 2.0-kb genomic fragment containing the proximal promoter of mouse Pkhd1 directs tissue-specific expression of a lacZ reporter gene in transgenic mice. LacZ is expressed in renal collecting ducts beginning during embryonic development but is not expressed in extrarenal tissues. The Pkhd1 promoter contains a binding site for the transcription factor hepatocyte nuclear factor (HNF)-1β, which is required for activity in transfected cells. Mutation of the HNF-1β-binding site abolishes the expression of the lacZ reporter gene in renal collecting ducts. Transgenes containing the 2.0-kb promoter and 2.7 kb of additional genomic sequence extending downstream to the second exon are expressed in the kidney, intrahepatic bile ducts, and male reproductive tract. This pattern overlaps with the endogenous expression of Pkhd1 and coincides with sites of expression of HNF-1β. We conclude that the proximal 2.0-kb promoter is sufficient for tissue-specific expression of Pkhd1 in renal collecting ducts in vivo and that HNF-1β is required for Pkhd1 promoter activity in collecting ducts. Additional genomic sequences located from exons 1-2 or elsewhere in the gene locus are required for expression in extrarenal tissues.

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