Thermo-labile stability of σH (Spo0H) in temperature-sensitive spo0H mutants of Bacillus subtilis can be suppressed by mutations in RNA polymerase β subunit

Gene ◽  
1999 ◽  
Vol 229 (1-2) ◽  
pp. 117-124 ◽  
Author(s):  
Yoshiaki Ohashi ◽  
Kei Sugimaru ◽  
Hideaki Nanamiya ◽  
Tamaki Sebata ◽  
Kei Asai ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Rna Polymerase ◽  
Temperature Sensitive ◽  
Β Subunit

scholarly journals Uncovering New Metabolic Capabilities of Bacillus subtilis Using Phenotype Profiling of Rifampin-Resistant rpoB Mutants

2007 ◽  
Vol 190 (3) ◽  
pp. 807-814 ◽  
Author(s):  
Amy E. Perkins ◽  
Wayne L. Nicholson
Keyword(s):  
Bacillus Subtilis ◽  
Rna Polymerase ◽  
Metabolic Profiling ◽  
Transcriptional Control ◽  
Β Subunit ◽  
Subunit Gene ◽  
Wild Type ◽  
Mutant Strains ◽  
Utilization Patterns ◽  
Flow Of Information

ABSTRACT RNA polymerase is a central macromolecular machine controlling the flow of information from genotype to phenotype, and insights into global transcriptional regulation can be gained by studying mutational perturbations in the enzyme. Mutations in the RNA polymerase β subunit gene rpoB causing resistance to rifampin (Rifr) in Bacillus subtilis were previously shown to lead to alterations in the expression of a number of global phenotypes known to be under transcriptional control, such as growth, competence for transformation, sporulation, and germination (H. Maughan, B. Galeano, and W. L. Nicholson, J. Bacteriol. 186:2481-2486, 2004). To better understand the global effects of rpoB mutations on metabolism, wild-type and 11 distinct congenic Rifr mutant strains of B. subtilis were tested for utilization of 95 substrates by use of Biolog GP2 MicroPlates. A number of alterations of substrate utilization patterns were observed in the Rifr mutants, including the utilization of novel substrates previously unknown in B. subtilis, such as gentiobiose, β-methyl-d-glucoside, and d-psicose. The results indicate that combining global metabolic profiling with mutations in RNA polymerase provides a system-wide approach for uncovering previously unknown metabolic capabilities and further understanding global transcriptional control circuitry in B. subtilis.

scholarly journals A Mutation in the Bacillus subtilis rsbU Gene That Limits RNA Synthesis during Sporulation

2017 ◽  
Vol 199 (14) ◽  
Author(s):  
David M. Rothstein ◽  
David Lazinski ◽  
Marcia S. Osburne ◽  
Abraham L. Sonenshein
Keyword(s):  
Bacillus Subtilis ◽  
Rna Polymerase ◽  
Sigma Factor ◽  
Rna Synthesis ◽  
Bacterial Species ◽  
Ethanol Stress ◽  
Temperature Sensitive ◽  
Content Type ◽  
Promoter Recognition ◽  
Bacillis Subtilis

ABSTRACT Mutants of Bacillis subtilis that are temperature sensitive for RNA synthesis during sporulation were isolated after selection with a 32P suicide agent. Whole-genome sequencing revealed that two of the mutants carried an identical lesion in the rsbU gene, which encodes a phosphatase that indirectly activates SigB, the stress-responsive RNA polymerase sigma factor. The mutation appeared to cause RsbU to be hyperactive, because the mutants were more resistant than the parent strain to ethanol stress. In support of this hypothesis, pseudorevertants that regained wild-type levels of sporulation at high temperature had secondary mutations that prevented expression of the mutant rsbU gene. The properties of these RsbU mutants support the idea that activation of SigB diminishes the bacterium's ability to sporulate. IMPORTANCE Most bacterial species encode multiple RNA polymerase promoter recognition subunits (sigma factors). Each sigma factor directs RNA polymerase to different sets of genes; each gene set typically encodes proteins important for responses to specific environmental conditions, such as changes in temperature, salt concentration, and nutrient availability. A selection for mutants of Bacillus subtilis that are temperature sensitive for RNA synthesis during sporulation unexpectedly yielded strains with a point mutation in rsbU, a gene that encodes a protein that normally activates sigma factor B (SigB) under conditions of salt stress. The mutation appears to cause RsbU, and therefore SigB, to be active inappropriately, thereby inhibiting, directly or indirectly, the ability of the cells to transcribe sporulation genes.

Non-coordinate synthesis of RNA polymerase ββ′ subunits in a temperature-sensitive β′-subunit mutant of Escherichia coli

1982 ◽  
Vol 188 (2) ◽  
pp. 173-178 ◽  
Author(s):  
Naoki Nakada ◽  
Koichi Yoshinaga ◽  
Akira Ishihama ◽  
Haruko Nagasawa-Fujimori
Keyword(s):  
Escherichia Coli ◽  
Rna Polymerase ◽  
Temperature Sensitive ◽  
Β Subunit

Zinc is associated with the β subunit of DNA-dependent RNA polymerase of Bacillus subtilis

Biochemistry ◽  
1977 ◽  
Vol 16 (13) ◽  
pp. 2880-2884 ◽  
Author(s):  
Shirley M. Halling ◽  
F. J. Sanchez-Anzaldo ◽  
Ryuji Fukuda ◽  
Roy H. Doi ◽  
Claude F. Meares
Keyword(s):  
Bacillus Subtilis ◽  
Rna Polymerase ◽  
Β Subunit

A temperature sensitive mutation of the β′-subunit of DNA-dependent RNA polymerase fromE. coliT16

FEBS Letters ◽  
1974 ◽  
Vol 48 (2) ◽  
pp. 241-245 ◽  
Author(s):  
Susan R. Panny ◽  
Alfred Heil ◽  
Barbara Mazus ◽  
Peter Palm ◽  
Wolfram Zillig
Keyword(s):  
Rna Polymerase ◽  
Temperature Sensitive ◽  
Β Subunit ◽  
Temperature Sensitive Mutation

scholarly journals Mutation in the Bacillus subtilis RNA Polymerase β′ Subunit Confers Resistance to Lipiarmycin

2006 ◽  
Vol 50 (1) ◽  
pp. 401-402 ◽  
Author(s):  
Maxime Gualtieri ◽  
Philippe Villain-Guillot ◽  
Jaqueline Latouche ◽  
Jean-Paul Leonetti ◽  
Lionel Bastide
Keyword(s):  
Bacillus Subtilis ◽  
Rna Polymerase ◽  
Β Subunit

scholarly journals Novel rpoB Mutations Conferring Rifampin Resistance on Bacillus subtilis: Global Effects on Growth, Competence, Sporulation, and Germination

2004 ◽  
Vol 186 (8) ◽  
pp. 2481-2486 ◽  
Author(s):  
Heather Maughan ◽  
Belinda Galeano ◽  
Wayne L. Nicholson
Keyword(s):  
Bacillus Subtilis ◽  
Amino Acid ◽  
Rna Polymerase ◽  
Amino Acid Change ◽  
Β Subunit ◽  
Resistance Mutations ◽  
Rifampin Resistance

ABSTRACT Previously, spontaneous rifampin resistance mutations were isolated in cluster I of the rpoB gene, resulting in amino acid replacements (Q469R, H482R, H482Y, or S487L) in the Bacillus subtilis RNA polymerase β subunit (W. L. Nicholson and H. Maughan, J. Bacteriol. 184:4936-4940, 2002). In this study, each amino acid change in the β subunit was observed to result in its own unique spectrum of effects on growth and various developmental events, including sporulation, germination, and competence for transformation. The results thus establish the important role played by the RNA polymerase β subunit, not only in the catalytic aspect of transcription, but also in the regulation of major developmental events in B. subtilis.

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