Research of microplastics in the gastrointestinal contents of sea turtles by the method of artificial digestion

2021 ◽  
Vol 350 ◽  
pp. S187-S188
Author(s):  
F. Lo Chiano ◽  
S. Rubini ◽  
M. Munari ◽  
L. Fiorentini ◽  
P. Bonilauri ◽  
...  
2019 ◽  
Vol 132 (2) ◽  
pp. 99-108
Author(s):  
RY Mejía-Radillo ◽  
AA Zavala-Norzagaray ◽  
JA Chávez-Medina ◽  
AA Aguirre ◽  
CM Escobedo-Bonilla
Keyword(s):  

2018 ◽  
Vol 589 ◽  
pp. 263-268 ◽  
Author(s):  
B Calmanovici ◽  
D Waayers ◽  
J Reisser ◽  
J Clifton ◽  
M Proietti

2019 ◽  
Vol 18 (1) ◽  
pp. 3 ◽  
Author(s):  
Charles J. Innis ◽  
Sarah Finn ◽  
Adam Kennedy ◽  
Elizabeth Burgess ◽  
Terry Norton ◽  
...  

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
William Robbins ◽  
Gary Conboy ◽  
Spencer Greenwood ◽  
Roland Schaper

Abstract Background Metastrongyloid parasites Angiostrongylus vasorum and Crenosoma vulpis infect wild and domestic canids and are important pathogens in dogs. Recent studies indicate that gastropod intermediate hosts infected with various metastrongyloids spontaneously shed infective third-stage larvae (L3) into the environment via feces and mucus under laboratory conditions. Shed L3 retain motility up to 120 days, but whether they retain infectivity was unknown. Methods To assess the infectivity of shed L3, the heart/lungs of six red foxes (Vulpes vulpes) were obtained from trappers in Newfoundland, Canada. Lungs were examined for first-stage larvae (L1) by the Baermann technique. A high number of viable A. vasorum L1 and a low number of C. vulpis L1 were recovered from one fox; these were used to infect naïve laboratory-raised Limax maximus. L3 recovered from slugs by artificial digestion were fed to two naïve purpose-bred research beagles (100 L3/dog). L1 shed by these two dogs was used to infect 546 L. maximus (2000–10,000 L1/slug). L3 shedding was induced by anesthetizing slugs in soda water and transferring them into warm (45 °C) tap water for at least 8 h. Shed L3 recovered from slugs were aliquoted on romaine lettuce in six-well tissue culture plates (80–500 L3/well) and stored at 16 °C/75% relative humidity. Four naïve research beagles were then exposed to 100 L3/dog from larvae stored for 0, 2, 4, or 8 weeks, respectively, after shedding. Results All four dogs began shedding C. vulpis L1 by 26–36 days post-infection (PI). All four dogs began shedding A. vasorum L1 by 50 days PI. Conclusions L3 infectivity for the definitive host was retained in both metastrongyloids, indicating the potential for natural infection in dogs through exposure from environmental contamination. As an additional exposure route, eating or licking plant or other material(s) contaminated with metastrongyloid L3 could dramatically increase the number of dogs at risk of infection from these parasites. Graphic Abstract


2021 ◽  
pp. 104063872110018
Author(s):  
Justin R. Perrault ◽  
Michael D. Arendt ◽  
Jeffrey A. Schwenter ◽  
Julia L. Byrd ◽  
Kathryn A. Tuxbury ◽  
...  

Blood glucose measurements provide important diagnostic information regarding stress, disease, and nutritional status. Glucose analytical methodologies include dry chemistry analysis (DCA) of plasma and point-of-care (POC) glucometer analysis of whole blood; however, these 2 methods differ in cost, required sample volume, and processing time. Because POC glucometers use built-in equations based on features of mammalian blood to convert whole blood measurements to plasma equivalent units, obtained glucose data must be compared and validated using gold-standard chemistry analytical methodology in reptiles. For in-water, trawl-captured, immature Kemp’s ridley sea turtles ( Lepidochelys kempii) from Georgia, USA, we observed significant, positive agreement between the 2 glucose determination methods; however, the glucometer overestimated glucose concentrations by 1.4 mmol/L on average in comparison to DCA and produced a wider range of results. The discordance of these results suggests that POC glucometer glucose data should be interpreted in the context of methodology- and brand-specific reference intervals along with concurrent packed cell volume data.


Author(s):  
Catherine Riaux-Gobin ◽  
Matt P. Ashworth ◽  
J.Patrick Kociolek ◽  
Damien Chevallier ◽  
Pablo Saenz-Agudelo ◽  
...  

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