scholarly journals Infectivity of gastropod-shed third-stage larvae of Angiostrongylus vasorum and Crenosoma vulpis to dogs

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
William Robbins ◽  
Gary Conboy ◽  
Spencer Greenwood ◽  
Roland Schaper
Keyword(s):  
Tap Water ◽  
Natural Infection ◽  
Angiostrongylus Vasorum ◽  
Intermediate Hosts ◽  
Red Foxes ◽  
Romaine Lettuce ◽  
Crenosoma Vulpis ◽  
Artificial Digestion ◽  
Third Stage ◽  
Limax Maximus

Abstract Background Metastrongyloid parasites Angiostrongylus vasorum and Crenosoma vulpis infect wild and domestic canids and are important pathogens in dogs. Recent studies indicate that gastropod intermediate hosts infected with various metastrongyloids spontaneously shed infective third-stage larvae (L3) into the environment via feces and mucus under laboratory conditions. Shed L3 retain motility up to 120 days, but whether they retain infectivity was unknown. Methods To assess the infectivity of shed L3, the heart/lungs of six red foxes (Vulpes vulpes) were obtained from trappers in Newfoundland, Canada. Lungs were examined for first-stage larvae (L1) by the Baermann technique. A high number of viable A. vasorum L1 and a low number of C. vulpis L1 were recovered from one fox; these were used to infect naïve laboratory-raised Limax maximus. L3 recovered from slugs by artificial digestion were fed to two naïve purpose-bred research beagles (100 L3/dog). L1 shed by these two dogs was used to infect 546 L. maximus (2000–10,000 L1/slug). L3 shedding was induced by anesthetizing slugs in soda water and transferring them into warm (45 °C) tap water for at least 8 h. Shed L3 recovered from slugs were aliquoted on romaine lettuce in six-well tissue culture plates (80–500 L3/well) and stored at 16 °C/75% relative humidity. Four naïve research beagles were then exposed to 100 L3/dog from larvae stored for 0, 2, 4, or 8 weeks, respectively, after shedding. Results All four dogs began shedding C. vulpis L1 by 26–36 days post-infection (PI). All four dogs began shedding A. vasorum L1 by 50 days PI. Conclusions L3 infectivity for the definitive host was retained in both metastrongyloids, indicating the potential for natural infection in dogs through exposure from environmental contamination. As an additional exposure route, eating or licking plant or other material(s) contaminated with metastrongyloid L3 could dramatically increase the number of dogs at risk of infection from these parasites. Graphic Abstract

scholarly journals Natural infection of Deroceras laeve (Mollusca: gastropoda) with metastrongylid larvae in a transmission focus of abdominal angiostrongyliasis

2002 ◽  
Vol 44 (1) ◽  
pp. 53-54 ◽  
Author(s):  
Rafael Lucyk MAURER ◽  
Carlos GRAEFF-TEIXEIRA ◽  
José Willibaldo THOMÉ ◽  
Luís Antônio CHIARADIA ◽  
Hiroko SUGAYA ◽  
...  
Keyword(s):  
Epidemiological Study ◽  
Natural Infection ◽  
Southern Brazil ◽  
Intermediate Hosts ◽  
Santa Catarina ◽  
Crop Pest ◽  
The Third ◽  
The Family ◽  
Third Stage ◽  
First Time

Angiostrongylus costaricensis is a nematode parasitic of rodents. Man may become infected by ingestion of the third stage larvae produced within the intermediate hosts, usually slugs from the family Veronicellidae. An epidemiological study carried out in a locality in southern Brazil (western Santa Catarina State) where these slugs are a crop pest and an important vector for A. costaricensis has documented for the first time the natural infection of Deroceras laeve with metastrongylid larvae. This small limacid slug is frequently found amid the folds of vegetable leaves and may be inadvertently ingested. Therefore D. laeve may have an important role in transmission of A. costaricensis to man.

scholarly journals Detection of Crenosoma spp., Angiostrongylus vasorum and Aelurostrongylus abstrusus in Gastropods in Eastern Austria

Pathogens ◽  
2020 ◽  
Vol 9 (12) ◽  
pp. 1046 ◽  
Author(s):  
Hans-Peter Fuehrer ◽  
Simone Morelli ◽  
Julian Bleicher ◽  
Thomas Brauchart ◽  
Mirjam Edler ◽  
...  
Keyword(s):  
At Risk ◽  
Veterinary Medicine ◽  
Sequence Analysis ◽  
Angiostrongylus Vasorum ◽  
Risk Of Infection ◽  
Aelurostrongylus Abstrusus ◽  
Crenosoma Vulpis ◽  
Arion Vulgaris ◽  
Eastern Austria ◽  
Limax Maximus

Canine and feline cardiorespiratory parasites are of utmost relevance in veterinary medicine. Key epizootiological information on major pet metastrongyloids, i.e., Angiostrongylus vasorum and Crenosoma vulpis infecting dogs, and Aelurostrongylus abstrusus and Troglostrongylus brevior infecting cats, is missing from Austria. This study investigated their occurrence in 1320 gastropods collected in the Austrian provinces of Styria, Burgenland, Lower Austria, and in metropolitan Vienna. Metastrongyloid larvae were microscopically detected in 25 samples, and sequence analysis confirmed the presence of metastrongyloids in nine samples, i.e., A. vasorum in one slug (Arion vulgaris) (0.07%), C. vulpis in five slugs (one Limax maximus and four A. vulgaris) (0.4%), A. abstrusus in two A. vulgaris (0.17%), and the hedgehog lungworm Crenosoma striatum was detected in one A. vulgaris. The present study confirms the enzooticity of major cardiorespiratory nematodes in Austria and that canine and feline populations are at risk of infection.

GIS-based environmental analysis of fox and canine lungworm distribution: an epidemiological study of Angiostrongylus vasorum and Crenosoma vulpis in red foxes from Slovakia

2018 ◽  
Vol 117 (2) ◽  
pp. 521-530 ◽  
Author(s):  
Viktória Čabanová ◽  
Martina Miterpáková ◽  
Michal Druga ◽  
Zuzana Hurníková ◽  
Daniela Valentová
Keyword(s):  
Epidemiological Study ◽  
Environmental Analysis ◽  
Angiostrongylus Vasorum ◽  
Red Foxes ◽  
Crenosoma Vulpis

Angiostrongylus vasorum and Crenosoma vulpis in red foxes (Vulpes vulpes) in Newfoundland, Canada

2004 ◽  
Vol 82 (1) ◽  
pp. 66-74 ◽  
Author(s):  
Rebecca A Jeffery ◽  
Murray W Lankester ◽  
Mike J McGrath ◽  
Hugh G Whitney
Keyword(s):  
Vulpes Vulpes ◽  
Pulmonary Arteries ◽  
Angiostrongylus Vasorum ◽  
Red Foxes ◽  
Crenosoma Vulpis ◽  
Host Sex ◽  
Winter Temperatures ◽  
The Right ◽  
Apparent Association ◽  
Dual Infections

Angiostrongylus vasorum and Crenosoma vulpis infect the pulmonary arteries and airways, respectively, of red foxes (Vulpes vulpes). Both are widespread in Europe, but within North America, A. vasorum occurs only on the island of Newfoundland. During 2000–2002, 366 red fox carcasses were examined from six regions of Newfoundland for the purpose of determining the distribution of both parasites, effects on the condition of their host, and whether infection with one affects that of the other. Crenosoma vulpis occurred island-wide with a prevalence of 87% and mean (±SE) intensity of 230 ± 20.8. Young-of-the-year red foxes had more C. vulpis (260 ± 39.4) than yearlings (91 ± 31.2) or adults (78 ± 41.1) (χ2 = 25.72, df = 2, p < 0.001), and numbers of adult worm were weakly related to fecal output of first-stage larvae (r2 = 0.20, p < 0.001) but not to host sex or body-fat index. Angiostrongylus vasorum occurred only in southeast Newfoundland where prevalence was 56% and mean intensity was 72 ± 7.6. Its distribution may be limited by cold, as it was absent from areas with mean winter temperatures below –4 °C. Intensity of adult A. vasorum was not related to host age, sex, larval output, or measures of body condition. Although referred to as a heartworm, 88% of adult worms were actually found in the pulmonary arteries rather than in the right ventricle. Furthermore, there was no apparent association between infections with the two parasites (Gc[1] = 0.10) even though 40% of red foxes had dual infections.

The life history and development of Amplicaecum robertsi, an ascaridoid nematode of the carpet python (Morelia spilotes variegatus). II. Growth and host specificity of larval stages in relation to the food chain

Parasitology ◽  
1963 ◽  
Vol 53 (1-2) ◽  
pp. 321-337 ◽  
Author(s):  
J. F. A. Sprent
Keyword(s):  
Life History ◽  
Host Specificity ◽  
Food Chain ◽  
Natural Infection ◽  
Indigenous Species ◽  
Intermediate Hosts ◽  
Second Stage ◽  
The Third ◽  
Wide Range ◽  
Third Stage

Observations are reported on the life history of Amplicaecum robertsi, in relation to the mode of infection as it occurs under natural conditions. Some of the natural intermediate hosts are listed, as compiled from observations on natural infection with third-stage larvae in Queensland mammals. Various natural intermediate hosts were experimentally infected by means of eggs, and observations on the growth and development of the larvae are described.It was found that the second-stage larvae have a wide range of hosts, including earthworms, snails, fish, tadpoles, reptiles, birds, and mammals. Development to the third-stage was only observed to occur in birds and mammals. Third-stage larvae exhibited a wide variation in the degree of growth attained in different animals. In birds, growth was slight, not extending beyond 3–4 mm.; in mammals, growth varied considerably in different species; it was greater in the indigenous species, reaching 70–80 mm. No development beyond the third-stage occurred, except in reptiles.Second-stage larvae in the tissues of invertebrates and lower vertebrates could be transferred by feeding to reptiles, birds and mammals. Development proceeded in these hosts to the same extent as occurred following egg infection.Third-stage larvae could be transferred to certain snakes and lizards by feeding liver of infected rodents, but third-stage larvae over 3 mm. could not be transferred to mammals. Third-stage larvae were found to be uninfective at all stages of growth for birds.The third moult occurred in the carpet snake when fed with third-stage larvae in the liver of Trichosurus caninus, Rattus assimilis and Melomys cervinipes infected 1 month previously. This was in contrast to previous findings with laboratory rodents, in which maturation of the third-stage larva took considerably longer.Besides the carpet snake, the third and fourth moult was observed to occur in the goanna (Varanus spp.), the blue-tongued lizard (Tiliqua scincoides) and the bearded dragon (Amphibolurus barbatus). No growth in length was observed and no eggs were evident. As far as is known, none of these lizards is a definitive host of A. robertsi.The results are discussed in relation to the food chain involved in the natural habitat of the carpet snake. The snake is depicted as the apex of a food pyramid, whose base comprises a variety of animals ranging from earthworms to herbivorous mammals. It is concluded that A. robertsi has adapted its life history in such a way as to enable the parasite to ascend the pyramid by transference through a series of predatory episodes, which culminate in the infection of the natural prey of the snake.The life history is thus regarded, not as a life cycle, but as a life pyramid; development proceeds according to a pattern of diminishing host-specificity. Host-specificity is wide at the base of the pyramid, so that second-stage larvae occur in a wide variety of paratenic hosts. Host specificity narrows at the second moult which may occur in birds and mammals. It narrows still further in the third stage, because this larva, though it will survive in reptiles, birds and mammals, will not grow to a length at which it is capable of further development in the snake, except in certain mammals. At the third moult, host specificity shifts to certain reptiles, but becomes eventually restrictive to the carpet snake, because this host alone appears to provide a suitable environment for maturation of the eggs.This work was financed by a research grant from the University of Queensland. The writer's sincere thanks are due to Miss Ann Pritchard and Mr J. Ballantyne for their valuable assistance.

scholarly journals The current situation of Angiostrongylus vasorum in Romania: a national questionnaire-based survey

2021 ◽  
Vol 17 (1) ◽  
Author(s):  
Georgiana Deak ◽  
Eduardo Berriatua ◽  
Andrei Daniel Mihalca
Keyword(s):  
Pulmonary Arteries ◽  
Clinical Manifestations ◽  
Epidemiological Studies ◽  
Domestic Dogs ◽  
Angiostrongylus Vasorum ◽  
Intermediate Hosts ◽  
Red Foxes ◽  
Neurological Signs ◽  
Wide Range ◽  
The Right

Abstract Background Angiostrongylus vasorum (Nematoda, Metastrongyloidea) is a vascular nematode that resides in the pulmonary arteries and the right side of the heart of a wide variety of carnivores, with an indirect life cycle using coprophagic gastropods as intermediate hosts. For domestic dogs, the infection with A. vasorum can be asymptomatic, but more frequently, it is associated with a wide range of clinical manifestations like cardio-respiratory signs, bleedings, neurological signs, and ocular problems which can lead to death when not treated accordingly. Angiostrongylosis was confirmed for the first time in Romania in red foxes (Vulpes vulpes) in 2017 and two years later a seroepidemiologic study was conducted among domestic dogs. However, to this date, no clinical canine angiostrongylosis cases were published in Romania. The aim of the present paper was to evaluate the knowledge about canine angiostrongylosis among veterinarians in Romania and to update the distribution of this disease using a national wide anonymous questionnaire. Results Overall, 147 unique responses were submitted, from 31 out of 42 counties. Twelve veterinarians (8%) from 8 counties (26%) acknowledged diagnosing a case of angiostrongylosis including 5 from the Bucharest and 1 from each of the remaining seven counties. All affected dogs had respiratory distress, 75% suffered cardiopathy, 16% coagulopathies and 8% neurological signs. Case diagnosis was based mostly on larval detection by coprology (67%) and serological antigen detection test (42%). Conclusions Romanian veterinarians are aware of canine angiostrongylosis and a significant number have clinical experience with the disease. Epidemiological studies are now needed to assess its distribution in the country, and further efforts are required to improve understanding of the disease, its diagnostic and treatment methods among veterinarians.

scholarly journals Larval development of the cat lungworm Aelurostrongylus abstrusus in the tropical freshwater snail Biomphalaria glabrata

2016 ◽  
Vol 2 ◽  
Author(s):  
EVA-MARIA ZOTTLER ◽  
MANUELA SCHNYDER
Keyword(s):  
Biomphalaria Glabrata ◽  
Freshwater Snail ◽  
Intermediate Hosts ◽  
Aelurostrongylus Abstrusus ◽  
Artificial Digestion ◽  
Third Stage ◽  
Stray Cat ◽  
Biomolecular Analysis ◽  
Freshwater Pulmonate Snail ◽  
Tropical Freshwater

SUMMARYAelurostrongylus abstrususis a worldwide occurring lungworm affecting felids. This metastrongyloid nematode has an indirect lifecycle relying on slugs and snails as intermediate hosts. In the present study the development of first-stage (L1) to third-stage larvae (L3) in the tropical freshwater pulmonate snailBiomphalaria glabratawas assessed. A total of 306 snails were individually exposed to 300A. abstrususL1, which were obtained from a naturally infected stray cat. The species was confirmed by biomolecular analysis. Second stage larvae (L2) and L3 were first isolated by artificial digestion of snails in the second and fourth week post exposure (wpe), respectively. From 8 wpe onwards, all larvae had developed into L3. Snails remained infected for up to 26 wpe. Only 0.4% of the L1 had pursued their development into L3, indicating low suitability of this artificial intermediate host for production of infectiveA. abstrususL3.

scholarly journals Faecal detection of the lungworms Crenosoma vulpis and Angiostrongylus vasorum and serological detection of A. vasorum in dogs from the Czech Republic

2017 ◽  
Vol 86 (4) ◽  
pp. 393-398 ◽  
Author(s):  
Markéta Hajnalová ◽  
Vlasta Svobodová ◽  
Manuela Schnyder ◽  
Roland Schaper ◽  
Miroslav Svoboda
Keyword(s):  
Czech Republic ◽  
Angiostrongylus Vasorum ◽  
Intermediate Hosts ◽  
Serum Samples ◽  
The Czech Republic ◽  
Specific Antibodies ◽  
Crenosoma Vulpis ◽  
Faecal Analysis ◽  
Faecal Samples ◽  
Circulating Antigen

Dogs are definitive hosts for the lungworms Crenosoma vulpis and Angiostrongylus vasorum. Intermediate hosts are gasteropoda (Mollusca). In the past, lungworms in dogs were rarely identified in the Czech Republic and in neighbouring countries. A total of 253 dogs were sampled for faecal analysis performed by the Baermann method for isolation of first stage larvae (L1) of lungworms. A total of 193 serum samples were collected and analysed by ELISA for the detection of a circulating antigen and specific antibodies against A. vasorum. All dog owners were asked to fill out a questionnaire. Nine dogs (4.7%, 95% Confidence Interval, CI: 2.2–8.7%) were positive for the A. vasorum circulating antigen, seven dogs (3.6%, CI: 1.5–7.3%) had specific antibodies against A. vasorum; of these, three animals (1.6%, CI: 0.3–4.5%) were positive for both ELISAs. Most of them were under the age of three years. Only three of 13 seropositive dogs were not healthy, namely, in one dog vomitus was observed and two dogs were pyretic. Faecal samples of 5 dogs were positive for C. vulpis L1 (2.0%, CI: 0.6–4.6%). Angiostrongylus vasorum L1 were found in the faecal sample of one dog (0.4%, CI: 0.01–2.2%). This study illustrates the wide-spread occurrence of lungworms in the Czech Republic. Disease awareness of lungworms among animal owners and veterinarians should be implemented.

scholarly journals Angiostrongylus vasorum in Domestic Dogs in Castilla y León, Iberian Peninsula, Spain

Animals ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1513
Author(s):  
Rodrigo Morchón ◽  
José Alberto Montoya-Alonso ◽  
José Ángel Sánchez-Agudo ◽  
Juan de Vicente-Bengochea ◽  
Xiomara Murcia-Martínez ◽  
...  
Keyword(s):  
Iberian Peninsula ◽  
Epidemiological Studies ◽  
Angiostrongylus Vasorum ◽  
Intermediate Hosts ◽  
Red Foxes ◽  
Stagnant Water ◽  
Castilla Y Leon ◽  
Wild Canids ◽  
Efficient Control ◽  
Irrigated Crops

Angiostrongylus vasorum is the causative agent of canine angiostrongylosis, a disease affecting domestic and wild canids. In Europe, it is an emerging disease, mainly reported in red foxes. In Spain, there are a few studies that address the prevalence and pathology of this disease. Castilla y León is the largest region of the Iberian Peninsula, whose extensive area is 94,224 km2; however, until now, there have been no epidemiological studies on this disease. Therefore, the aim of this study was to analyze the presence of antigens of A. vasorum in 1475 dogs from Castilla y León, showing an overall prevalence of 0.75%. The infected dogs were mainly outdoors, guard and hunting breed dogs and living in locations with mild climates close to areas of high edaphic humidity, such as stagnant water, irrigated crops or riverbanks, with the vegetation dominated by alders, holm oak and gall oak forests, where the intermediate hosts develop. It is necessary to carry out more in-depth studies on the epidemiology and pathology of this disease in Spain and Europe in order to carry out efficient control in both domestic and wild animals.

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