1 Determination of DNA Base Composition

1985 ◽  
pp. 1-31 ◽  
Author(s):  
John L. Johnson
1966 ◽  
Vol 16 (1) ◽  
pp. 107-113 ◽  
Author(s):  
P.C. Huang ◽  
Eugene Rosenberg

2007 ◽  
Vol 28 (3) ◽  
pp. 366-372 ◽  
Author(s):  
Ngoc-Phuc Hua ◽  
Takeshi Naganuma

1976 ◽  
Vol 31 (9-10) ◽  
pp. 554-557 ◽  
Author(s):  
Hans-Joachim Breter ◽  
Ferdinand Hundt ◽  
Rudolf K. Zahn

Abstract The (adenine + thymine)/(guanine + cytosine) base ratios of 19 species of adriatic sponges have been determined by high-pressure liquid cation-exchange chromatography. The base ratios vary from 1.49 (Mycale massa) to 0.63 (Hippospongia communis) according to an (A+T) content of 59.7 and 38.6 mol%, respectively. The DNAs of sponges of the order Keratosa showed marked differences in their (A +T) contents (39.5 to 58.8 mol%) whereas those of Tetractinellida and Halichondrina were nearly identical (39.3 to 40.8 and 49.5 to 49.8 mol%, respectively). The 5-methylcytosine (5MC) content was determined in 8 sponge DNAs by a semiquantitative method. The values differed from 0.8 to 2.2 mol% of 5MC.


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