A tandem solid phase extraction, reversed-phase HPLC method for determining SDZ WAG 994 in dog, monkey and rat blood

1997 ◽  
Vol 15 (6) ◽  
pp. 749-758 ◽  
Author(s):  
Jeffrey A. Cramer ◽  
Susan J. Pasternak ◽  
Lawrence V. Athill
Keyword(s):  
Solid Phase Extraction ◽  
Solid Phase ◽  
Reversed Phase ◽  
Hplc Method ◽  
Phase Extraction ◽  
Reversed Phase Hplc ◽  
Rat Blood

scholarly journals Determination of Degradation Products of Doxercalciferol by Solid-Phase Extraction and Reversed-Phase HPLC

2013 ◽  
Vol 52 (6) ◽  
pp. 520-525
Author(s):  
N. Simonzadeh ◽  
B. Ronsen ◽  
S. Upadhyaya ◽  
E. Wilkinson ◽  
K. Kanesvaran ◽  
...  
Keyword(s):  
Solid Phase Extraction ◽  
Solid Phase ◽  
Degradation Products ◽  
Reversed Phase ◽  
Phase Extraction ◽  
Reversed Phase Hplc

scholarly journals Determination of urinary free cortisol by HPLC

1997 ◽  
Vol 43 (8) ◽  
pp. 1386-1391 ◽  
Author(s):  
Ursula Turpeinen ◽  
Helene Markkanen ◽  
Matti Välimäki ◽  
Ulf-Håkan Stenman
Keyword(s):  
Solid Phase Extraction ◽  
Mobile Phase ◽  
Solid Phase ◽  
Internal Standard ◽  
Reversed Phase ◽  
Hplc Method ◽  
Extraction Column ◽  
Phase Extraction ◽  
Free Cortisol

Abstract We here report a reversed-phase HPLC method for the determination of free cortisol in human urine, using methylprednisolone as the internal standard. Before chromatography, samples were extracted with a C18 solid-phase extraction column and the steroids were separated on a LiChrospher 100 C18 column with a mobile phase of methanol/acetonitrile/water (43/3/54 by vol). Linearity, precision, and accuracy of the method were established. The detection limit was 10 pmol of cortisol, and total CVs were <8%. With various solid-phase extraction columns the recovery of cortisol was 36–97%; recovery of the internal standard was 43–85%. Study of interference by 6 other steroids and metabolites and 24 drugs showed that carbamazepine and digoxin partly overlapped with cortisol, but this interference could be reduced by modification of the mobile phase. The HPLC method was compared with an RIA and an automated immunoassay method. The results obtained by HPLC averaged 40% of the RIA values.

A Simple and Quick Solid Phase Extraction and Reversed Phase HPLC Analysis of Some Tropane Alkaloids in Feedstuffs and Biological Samples

1993 ◽  
Vol 16 (5) ◽  
pp. 975-998 ◽  
Author(s):  
I. N. Papadoyannis ◽  
V. F. Samanidou ◽  
G. A. Theodoridis ◽  
G. S. Vasilikiotis ◽  
G. J. M. Van Kempen ◽  
...  
Keyword(s):  
Solid Phase Extraction ◽  
Biological Samples ◽  
Hplc Analysis ◽  
Solid Phase ◽  
Tropane Alkaloids ◽  
Reversed Phase ◽  
Phase Extraction ◽  
Reversed Phase Hplc

scholarly journals Analysis of low-dose benzodiazepines by HPLC with automated solid-phase extraction

1996 ◽  
Vol 42 (9) ◽  
pp. 1412-1416 ◽  
Author(s):  
K K Akerman ◽  
J Jolkkonen ◽  
M Parviainen ◽  
I Penttilä
Keyword(s):  
Solid Phase Extraction ◽  
Clinical Laboratory ◽  
Solid Phase ◽  
Antidepressant Drugs ◽  
Reversed Phase ◽  
Hplc Method ◽  
Array Detector ◽  
Therapeutic Drug ◽  
Phase Extraction ◽  
Automated Solid Phase Extraction

Abstract We describe a simple, specific, and sensitive reversed-phase HPLC method with automated solid-phase extraction (SPE) for analyzing alprazolam, clonazepam, and nitrazepam concentrations in human serum or plasma. We prepare samples and calibrators with an automated sample preparer, using 100-mg Bond-Elut C18 SPE columns. The HPLC method uses isocratic elution with acetonitrile:methanol:dipotassium hydrogen phosphate, 10 nmol/L, pH 3.7 (30:2:100 by vol), at a flow rate of 1.5 mL/min to separate the drugs. We detect the benzodiazepines with diode-array detector at 240 nm; analyses of peak purity are performed at 210-365 nm. The recoveries were between 94% and 100%. The intraassay and interassay CVs were between 1.0% and 4.1%. The detection limit is 5 nmol/L. The antiepileptic and antidepressant drugs tested did not interfere with the assay. We developed the method for use in a clinical laboratory for therapeutic drug monitoring.

An Optimized Method for the quantitation of Carboxyamidotriazole (CAI) in Human Plasma with Solid Phase Extraction and reversed Phase HPLC

1997 ◽  
Vol 30 (11) ◽  
pp. 2009-2021 ◽  
Author(s):  
Bradley R. Simmons ◽  
Kenneth S. Bauer ◽  
Natalie A. McCall ◽  
Elise C. Kohn ◽  
William D. Figg
Keyword(s):  
Solid Phase Extraction ◽  
Human Plasma ◽  
Solid Phase ◽  
Reversed Phase ◽  
Phase Extraction ◽  
Reversed Phase Hplc
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