A solid phase extraction reversed-phase HPLC method for the simultaneous determination of methoprene, permethrin and selected metabolites in rat plasma and urine

2001 ◽  
Vol 15 (7) ◽  
pp. 464-470 ◽  
Author(s):  
Aqel W. Abu-Qare ◽  
Mohamed B. Abou-Donia
Keyword(s):  
Solid Phase Extraction ◽  
Simultaneous Determination ◽  
Solid Phase ◽  
Rat Plasma ◽  
Reversed Phase ◽  
Hplc Method ◽  
Phase Extraction ◽  
Reversed Phase Hplc

scholarly journals Determination of Degradation Products of Doxercalciferol by Solid-Phase Extraction and Reversed-Phase HPLC

2013 ◽  
Vol 52 (6) ◽  
pp. 520-525
Author(s):  
N. Simonzadeh ◽  
B. Ronsen ◽  
S. Upadhyaya ◽  
E. Wilkinson ◽  
K. Kanesvaran ◽  
...  
Keyword(s):  
Solid Phase Extraction ◽  
Solid Phase ◽  
Degradation Products ◽  
Reversed Phase ◽  
Phase Extraction ◽  
Reversed Phase Hplc

scholarly journals Determination of urinary free cortisol by HPLC

1997 ◽  
Vol 43 (8) ◽  
pp. 1386-1391 ◽  
Author(s):  
Ursula Turpeinen ◽  
Helene Markkanen ◽  
Matti Välimäki ◽  
Ulf-Håkan Stenman
Keyword(s):  
Solid Phase Extraction ◽  
Mobile Phase ◽  
Solid Phase ◽  
Internal Standard ◽  
Reversed Phase ◽  
Hplc Method ◽  
Extraction Column ◽  
Phase Extraction ◽  
Free Cortisol

Abstract We here report a reversed-phase HPLC method for the determination of free cortisol in human urine, using methylprednisolone as the internal standard. Before chromatography, samples were extracted with a C18 solid-phase extraction column and the steroids were separated on a LiChrospher 100 C18 column with a mobile phase of methanol/acetonitrile/water (43/3/54 by vol). Linearity, precision, and accuracy of the method were established. The detection limit was 10 pmol of cortisol, and total CVs were <8%. With various solid-phase extraction columns the recovery of cortisol was 36–97%; recovery of the internal standard was 43–85%. Study of interference by 6 other steroids and metabolites and 24 drugs showed that carbamazepine and digoxin partly overlapped with cortisol, but this interference could be reduced by modification of the mobile phase. The HPLC method was compared with an RIA and an automated immunoassay method. The results obtained by HPLC averaged 40% of the RIA values.

Sign in / Sign up

Export Citation Format

Share Document