Constitutive expression of a phenylalanine ammonia-lyase gene from Stylosanthes humilis in transgenic tobacco leads to enhanced disease resistance but impaired plant growth

2002 ◽  
Vol 60 (6) ◽  
pp. 275-282 ◽  
Author(s):  
H Way
Keyword(s):  
Disease Resistance ◽  
Plant Growth ◽  
Transgenic Tobacco ◽  
Phenylalanine Ammonia Lyase ◽  
Constitutive Expression ◽  
Stylosanthes Humilis ◽  
Phenylalanine Ammonia Lyase Gene

Rice phenylalanine ammonia-lyase gene OsPAL4 is associated with broad spectrum disease resistance

2014 ◽  
Vol 87 (3) ◽  
pp. 273-286 ◽  
Author(s):  
Bradley W. Tonnessen ◽  
Patricia Manosalva ◽  
Jillian M. Lang ◽  
Marietta Baraoidan ◽  
Alicia Bordeos ◽  
...  
Keyword(s):  
Disease Resistance ◽  
Broad Spectrum ◽  
Phenylalanine Ammonia Lyase ◽  
Phenylalanine Ammonia Lyase Gene

scholarly journals Constitutive Expression of hrap Gene in Transgenic Tobacco Plant Enhances Resistance Against Virulent Bacterial Pathogens by Induction of a Hypersensitive Response

2002 ◽  
Vol 15 (8) ◽  
pp. 764-773 ◽  
Author(s):  
Mang-jye Ger ◽  
Cheng-hsien Chen ◽  
Shaw-yhi Hwang ◽  
Hsiang-en Huang ◽  
Appa Rao Podile ◽  
...  
Keyword(s):  
Disease Resistance ◽  
Transgenic Tobacco ◽  
Pseudomonas Syringae ◽  
Hypersensitive Response ◽  
Plant Disease Resistance ◽  
Constitutive Expression ◽  
Sweet Pepper ◽  
Blue Staining ◽  
Wild Type ◽  
Pathogen Infection

Hypersensitive response-assisting protein (HRAP) has been previously reported as an amphipathic plant protein isolated from sweet pepper that intensifies the harpinPss-mediated hypersensitive response (HR). The hrap gene has no appreciable similarity to any other known sequences, and its activity can be rapidly induced by incompatible pathogen infection. To assess the function of the hrap gene in plant disease resistance, the CaMV 35S promoter was used to express sweet pepper hrap in transgenic tobacco. Compared with wild-type tobacco, transgenic tobacco plants exhibit more sensitivity to harpinPss and show resistance to virulent pathogens (Pseudomonas syringae pv. tabaci and Erwinia carotovora subsp. carotovora). This disease resistance of transgenic tobacco does not originate from a constitutive HR, because endogenous level of salicylic acid and hsr203J mRNA showed similarities in transgenic and wild-type tobacco under noninfected conditions. However, following a virulent pathogen infection in hrap transgenic tobacco, hsr203J was rapidly induced and a micro-HR necrosis was visualized by trypan blue staining in the infiltration area. Consequently, we suggest that the disease resistance of transgenic plants may result from the induction of a HR by a virulent pathogen infection.

scholarly journals Demonstration of improved plant growth and biomass production by At1g 73160 promoter-controlled root-preferential expression of AtGA20ox gene

2020 ◽  
Author(s):  
Linh Khanh Ly ◽  
Thao Phuong Bui ◽  
Phat Tien Do ◽  
Anh Van Thi Le ◽  
Phong Van Nguyen ◽  
...  
Keyword(s):  
Transgenic Plants ◽  
Plant Growth ◽  
Transgenic Tobacco ◽  
Plant Species ◽  
Leaf Shape ◽  
Ectopic Expression ◽  
Constitutive Expression ◽  
Stem Length ◽  
Melia Azedarach ◽  
Significant Difference

Abstract Background: Overexpression of GA20 oxidase gene has been a recent trend for improving plant growth and biomass. Constitutive expression of GA20ox has successfully improved plant growth and biomass in several plant species. However, the constitutive expression of this gene causes side-effects, such as reduced leaf size and stem diameters, etc. To avoid these effects, different tissue-specific promoters had been identified and employed for GA20ox overexpression. In this study, we demonstrate the potential of At1g, a root-preferential promoter, for GA20ox expression to enhance plant biomass in tobacco and Melia azedarach.Results: We examined the utility of At1g promoter to drive the expression of GUS (β-1,4-glucuronidase) reporter and GA20ox genes in tobacco and Melia azedarach. Histochemical GUS assays in tobacco showed that At1g was a root-preferential promoter whose expression was particularly strong in root tips. The ectopic expression of AtGA20ox gene under the control of At1g promoter showed the improved plant growth and biomass of both tobacco and M. azedarach transgenic plants compared to wild-type (WT) control plants. Stem length as well as stem and root fresh weights increased by up to 1.5-3 folds in transgenic tobacco and 2 folds in transgenic M. azedarach. Both tobacco and M. azedarach transgenic plants showed increases in the root xylem width, xylem over phloem ratio by 50%-100% as compared to WT plants. Importantly, no significant difference in the leaf shape and size was observed between At1g::AtGA20ox transgenic and WT plants. Moreover, transgenic M. azedarach showed a 135% increase in stem diameter even though no change was found in transgenic tobacco.Conclusions: These results demonstrate the great utility of At1g promoter, when driving AtGA20ox gene, for growth and biomass improvements in woody plants and potentially some other plant species.

Demonstration of improved plant growth and biomass by At1g promoter-controlled root-preferential expression of AtGA20ox gene

2020 ◽  
Author(s):  
Linh Khanh Ly ◽  
Thao Phuong Bui ◽  
Phat Tien Do ◽  
Anh Van Thi Le ◽  
Phong Van Nguyen ◽  
...  
Keyword(s):  
Transgenic Plants ◽  
Plant Growth ◽  
Transgenic Tobacco ◽  
Plant Species ◽  
Leaf Shape ◽  
Ectopic Expression ◽  
Constitutive Expression ◽  
Stem Length ◽  
Melia Azedarach ◽  
Significant Difference

Abstract Background: Overexpression of GA20 oxidase gene has been a recent trend for improving plant growth and biomass. Constitutive expression of GA20ox has successfully improved plant growth and biomass in several plant species. However, the constitutive expression of this gene causes side-effects, such as reduced leaf size and stem diameters, etc. To avoid these effects, different tissue-specific promoters had been identified and employed for GA20ox overexpression. In this study, we demonstrate the potential of At1g, a root-preferential promoter, for GA20ox expression to enhance plant biomass.Results: We examined the utility of At1g promoter to drive the expression of GUS (b-glucuronidase) reporter and GA20ox genes in tobacco and Melia azedarach. Histochemical GUS assays in tobacco showed that At1g was a root-preferential promoter whose expression was particularly strong in root tips. The ectopic expression of AtGA20ox gene under the control of At1g promoter showed the improved plant growth and biomass of both tobacco and M. azedarach transgenic plants compared to wild-type (WT) control plants. Stem length as well as stem and root fresh weights increased by up to 1.5-3 folds in transgenic tobacco and 2 folds in transgenic M. azedarach. Both tobacco and M. azedarach transgenic plants showed increases in the root xylem width, xylem over phloem ratio by 50%-100% as compared to WT plants. Importantly, no significant difference in the leaf shape and size was observed between At1g::AtGA20ox transgenic and WT plants. Moreover, transgenic M. azedarach showed a 135% increase in stem diameter even though no change was found in transgenic tobacco.Conclusions: These results demonstrate the great utility of At1g promoter, when driving AtGA20ox gene, for growth and biomass improvements in woody plants and potentially some other plant species.

scholarly journals Demonstration of improved plant growth and biomass production by At1g73160 promoter-controlled root-preferential expression of the AtGA20ox gene

2020 ◽  
Author(s):  
Linh Khanh Ly ◽  
Thao Phuong Bui ◽  
Phat Tien Do ◽  
Anh Van Thi Le ◽  
Phong Van Nguyen ◽  
...  
Keyword(s):  
Transgenic Plants ◽  
Plant Growth ◽  
Transgenic Tobacco ◽  
Plant Species ◽  
Plant Biomass ◽  
Leaf Shape ◽  
Ectopic Expression ◽  
Constitutive Expression ◽  
Stem Length ◽  
Melia Azedarach

Abstract Background: Overexpression of the GA20 oxidase gene has been a recent trend for improving plant growth and biomass. Constitutive expression of GA20ox has successfully improved plant growth and biomass in several plant species. However, the constitutive expression of this gene causes side-effects, such as reduced leaf size and stem diameters. To avoid these effects, different tissue-specific promoters were identified and employed for GA20ox overexpression. In this study, we demonstrated the potential of At1g, a root-preferential promoter, for GA20ox expression to enhance plant biomass in tobacco and Melia azedarach.Results: We examined the utility of the At1g promoter to drive the expression of a GUS (β-1,4-glucuronidase) reporter and the GA20ox gene in tobacco and Melia azedarach. Histochemical GUS assays in tobacco showed that At1g was a root-preferential promoter whose expression was particularly strong in root tips. The ectopic expression of the AtGA20ox gene under the control of the At1g promoter showed improved plant growth and biomass of both tobacco and M. azedarach transgenic plants compared to wild-type (WT) control plants. Stem length as well as stem and root fresh weights increased by up to 1.5-3 folds in transgenic tobacco and 2-fold in transgenic M. azedarach. Both tobacco and M. azedarach transgenic plants showed increases in the root xylem width and xylem over phloem ratio by 50%–100% compared to WT plants. Importantly, no significant differences in the leaf shape or size were observed between the At1g::AtGA20ox transgenic and WT plants. Moreover, transgenic M. azedarach showed a 135% increase in stem diameter although no change was found in transgenic tobacco.Conclusions: These results demonstrate the great utility of the At1g promoter for driving the AtGA20ox gene to induce growth and biomass improvements in woody plants and potentially some other plant species.

scholarly journals Phenylpropanoid compounds and disease resistance in transgenic tobacco with altered expression of l -phenylalanine ammonia-lyase

2003 ◽  
Vol 64 (1) ◽  
pp. 153-161 ◽  
Author(s):  
Gail L. Shadle ◽  
S.Varsha Wesley ◽  
Kenneth L. Korth ◽  
Fang Chen ◽  
Chris Lamb ◽  
...  
Keyword(s):  
Disease Resistance ◽  
Transgenic Tobacco ◽  
Phenylalanine Ammonia Lyase ◽  
Altered Expression
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