Effects of aging on the susceptibility to the toxic effects of cyclosporin A in rats. Changes in liver glutathione and antioxidant enzymes

2001 ◽  
Vol 30 (8) ◽  
pp. 836-845 ◽  
Author(s):  
J Palomero
Keyword(s):  
Antioxidant Enzymes ◽  
Cyclosporin A ◽  
Toxic Effects ◽  
Liver Glutathione ◽  
Effects Of Aging

scholarly journals Further evidence that cyclosporin A protects mitochondria from calcium overload by inhibiting a matrix peptidyl-prolyl cis-trans isomerase. Implications for the immunosuppressive and toxic effects of cyclosporin

1991 ◽  
Vol 274 (2) ◽  
pp. 611-614 ◽  
Author(s):  
E J Griffiths ◽  
A P Halestrap
Keyword(s):  
Cyclosporin A ◽  
Mechanism Of Action ◽  
Adenine Nucleotide ◽  
Calcium Overload ◽  
Toxic Effects ◽  
The Novel ◽  
Fk 506 ◽  
Previous Hypothesis ◽  
Liver And Kidney ◽  
Pore Opening

The Ki values of cyclosporins A, G and H for the peptidyl-prolyl cis-trans isomerase (PPIase) of liver and heart mitochondria are about 2, 20 and 500 nM respectively. This parallels their profile as inhibitors of non-specific pore opening of mitochondria induced by supraphysiological Ca2+ concentrations. The novel immunosuppressant FK-506 gave little inhibition of either process at 5 microM. These data support our previous hypothesis [Halestrap & Davidson (1990) Biochem. J. 268, 153-160] that pore opening involves an interaction between matrix PPIase and the adenine nucleotide translocase. It is suggested that this model may help to clarify the mechanism of action of cyclosporin as an immunosuppressant and its toxic effects on the liver and kidney following prolonged therapy.

714 The Effects of Aging and Training on Glutathione Status and Antioxidant Enzymes in Rat Skeletal Muscle

1993 ◽  
Vol 25 (Supplement) ◽  
pp. S129
Author(s):  
C. Leeuwenburgh ◽  
E. W. Mitchell ◽  
R. Chandwaney ◽  
S. Leichtweis ◽  
L. L. Ji
Keyword(s):  
Skeletal Muscle ◽  
Antioxidant Enzymes ◽  
Rat Skeletal Muscle ◽  
Glutathione Status ◽  
Effects Of Aging ◽  
And Training

Susceptibility to the toxic effects of cyclosporin in the rat: effects of aging

2001 ◽  
Vol 34 ◽  
pp. 202
Author(s):  
J. Palomero ◽  
A. Galan ◽  
J. Gonzalez-Buitrago ◽  
M. Munoz ◽  
M. Tunon ◽  
...  
Keyword(s):  
Toxic Effects ◽  
Effects Of Aging

scholarly journals Effects of Aging and Cyclosporin A on Collagen Turnover in Human Gingiva

2009 ◽  
Vol 3 (1) ◽  
pp. 219-226 ◽  
Author(s):  
N Gagliano ◽  
F Costa ◽  
G.M Tartaglia ◽  
L Pettinari ◽  
F Grizzi ◽  
...  
Keyword(s):  
Cyclosporin A ◽  
Collagen Content ◽  
Gingival Fibroblasts ◽  
Collagen Turnover ◽  
Dot Blot ◽  
Aged People ◽  
Human Gingiva ◽  
Young Subjects ◽  
Effects Of Aging ◽  
Sirius Red

Background: We aimed at characterizing the aging gingiva analyzing: i) collagen content and turnover in human gingival tissues and fibroblasts obtained from healthy young and aging subjects. ii) the effect of cyclosporin A administration in human cultured gingival fibroblasts obtained from aging compared to young subjects. Methods: Morphological analysis was performed on haematoxylin-eosin and Sirius red stained paraffin-embedded gingival biopsies from young and aging healthy subjects. The expression of the main genes and proteins involved in collagen turnover were determined by real time PCR, dot blot and SDS-zymography on cultured young and aging gingival fibroblasts, and after cyclosporin A administration. Results: Our results suggest that in healthy aged people, gingival connective tissue is characterized by a similar collagen content and turnover. Collagen turnover pathways are similarly affected by cyclosporin A treatment in young and aging gingival fibroblasts. Conclusions: Cyclosporin A administration affects gingival collagen turnover pathways in young and aging fibroblasts at the same extent, suggesting that during aging cyclosporin A administration is not related to relevant collagen turnover modifications.

scholarly journals The influence of natural dicarbonils on the antioxidant enzymes activity in vitro and in vivo

2012 ◽  
Vol 58 (6) ◽  
pp. 727-736 ◽  
Author(s):  
V.Z. Lankin ◽  
G.G. Konovalova ◽  
A.K. Tikhaze ◽  
L.V. Nedosugova
Keyword(s):  
Oxidative Stress ◽  
Diabetes Mellitus ◽  
Antioxidant Enzymes ◽  
Carbonyl Stress ◽  
Diabetic Patients ◽  
Glutathione S Transferase ◽  
Liver Glutathione ◽  
Bovine Erythrocytes

Natural dicarbonyls, which may be accumulated during oxidative stress in atherosclerosis (e.g. malondialdehyde) or carbonyl stress in diabetes mellitus (glyoxal and methylglyoxal) effectively inhibited the activities of commercial preparations of antioxidant enzymes: catalase, Cu,Zn-superoxide dismutase (Cu,Zn-SOD) and Se-contained glutathione peroxidase from human and bovine erythrocytes and also rat liver glutathione-S-transferase. After incubation of human erythrocytes with 10 mM of each investigated dicarbonyls the decrease of intracellular Cu,Zn-SOD was observed. The decreased activity of erythrocyte Cu,Zn-SOD was also detected in diabetic patients with carbohydrate metabolism disturbance but effective sugar-lowered therapy was accompanied by the increase of this enzyme activity. The increase of erythrocytes activity of Cu,Zn-SOD of diabetic patients theated with metformin (which may utilize methylglyoxal) was higher than in erythrocytase of diabetic patients subjected to traditional therapy.

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