Immobilized enzyme reaction controlled by magnetic heating: γ-Fe2O3-loaded thermosensitive polymer gels consisting of N-isopropylacrylamide and acrylamide

1997 ◽  
Vol 83 (2) ◽  
pp. 152-156 ◽  
Author(s):  
Fujio Takahashi ◽  
Yasuzo Sakai ◽  
Yohsuke Mizutani
Keyword(s):  
Immobilized Enzyme ◽  
Enzyme Reaction ◽  
Polymer Gels ◽  
Thermosensitive Polymer ◽  
Magnetic Heating

A stopped-flow clinical analyzer in which immobilized-enzyme reaction loops are used.

1977 ◽  
Vol 23 (6) ◽  
pp. 1033-1036 ◽  
Author(s):  
M D Joseph ◽  
D J Kasprzak ◽  
S R Crouch
Keyword(s):  
Immobilized Enzyme ◽  
Enzymatic Reaction ◽  
Enzyme Reaction ◽  
Fixed Time ◽  
Stopped Flow ◽  
Good Precision ◽  
Indicator Reaction ◽  
Flow Mixing ◽  
Kinetic Mode

Abstract A stopped-flow clinical analyzer is described that makes use of a reaction loop containing immobilized enzyme(s) for the determination of the analyte/substrate. The analyzer has been evaluated by determining glucose with immobilized glucose oxidase. The stopped-flow mixing system was constructed at a current cost of less than $500. The analyzer separates the enzymatic reaction from a followup, spectrophotometric indicator reaction. This separation allows the enzymatic reaction to be used in either a fixed-time, kinetic mode or in an equilibrium mode. Likewise, the indicator reaction can be used in either mode. Results for glucose in blood serum indicate that good precision and accuracy can be obtained.

Immobilized enzyme reaction stability: Attrition of the support material

1974 ◽  
Vol 16 (3) ◽  
pp. 333-343 ◽  
Author(s):  
D. L. Regan ◽  
P. Dunnill ◽  
M. D. Lilly
Keyword(s):  
Immobilized Enzyme ◽  
Enzyme Reaction ◽  
Support Material
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