Determination of nitrate ion at the part per billion level in environmental samples with a continuous flow immobilized enzyme reaction

1976 ◽  
Vol 48 (7) ◽  
pp. 954-958 ◽  
Author(s):  
David R. Senn ◽  
Peter W. Carr
Keyword(s):  
Continuous Flow ◽  
Environmental Samples ◽  
Immobilized Enzyme ◽  
Enzyme Reaction ◽  
Nitrate Ion

A stopped-flow clinical analyzer in which immobilized-enzyme reaction loops are used.

1977 ◽  
Vol 23 (6) ◽  
pp. 1033-1036 ◽  
Author(s):  
M D Joseph ◽  
D J Kasprzak ◽  
S R Crouch
Keyword(s):  
Immobilized Enzyme ◽  
Enzymatic Reaction ◽  
Enzyme Reaction ◽  
Fixed Time ◽  
Stopped Flow ◽  
Good Precision ◽  
Indicator Reaction ◽  
Flow Mixing ◽  
Kinetic Mode

Abstract A stopped-flow clinical analyzer is described that makes use of a reaction loop containing immobilized enzyme(s) for the determination of the analyte/substrate. The analyzer has been evaluated by determining glucose with immobilized glucose oxidase. The stopped-flow mixing system was constructed at a current cost of less than $500. The analyzer separates the enzymatic reaction from a followup, spectrophotometric indicator reaction. This separation allows the enzymatic reaction to be used in either a fixed-time, kinetic mode or in an equilibrium mode. Likewise, the indicator reaction can be used in either mode. Results for glucose in blood serum indicate that good precision and accuracy can be obtained.

The use of immobilized enzyme reactors in continuous-flow analyzers for the determination of glucose, urea, and uric acid.

1979 ◽  
Vol 25 (10) ◽  
pp. 1744-1748 ◽  
Author(s):  
R Chirillo ◽  
G Caenaro ◽  
B Pavan ◽  
A Pin
Keyword(s):  
Uric Acid ◽  
Continuous Flow ◽  
Immobilized Enzyme ◽  
Immobilized Enzymes ◽  
Free Enzyme ◽  
Serum Samples ◽  
Assay Sensitivity ◽  
Enzyme Reactors ◽  
Immobilized Enzyme Reactors

Abstract We describe the use of immobilized enzymes in assay methods for the determination of glucose with glucose oxidase, uric acid with uricase, and urea with urease in serum samples. The enzyme reactor tubes were adapted to continuous-flow analyzers (Technicon AA II, SMA 12/60, and SMAC) used in routine laboratory determinations, and results with their use were compared to those from assays involving soluble enzymes. We substituted the reactors for the free enzyme reagents in the respective channels of the SMA 12/60 and SMAC, without modifying the parameters of the remaining channels. We compared assay sensitivity, precision, and carryover for immobilized and conventional liquid enzymes. Immobilized enzyme reactors provide accurate, reliable, convenient, and economical alternatives to the use of free enzyme reagents in these systems.

Determination of Nitrate Ion in Cheese and Sake

2020 ◽  
Vol 61 (6) ◽  
pp. 229-234
Author(s):  
Takahiro Sasaki ◽  
Shoichi Tahara ◽  
Ritsuko Toyohara ◽  
Mari Morikawa ◽  
Narue Sakamaki ◽  
...  
Keyword(s):  
Nitrate Ion
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