Determination of immunity to measles virus in young adults: comparative evaluation of a commercial enzyme immunoassay and the hemagglutination inhibition techniques

1996 ◽  
Vol 7 (1) ◽  
pp. 1-6 ◽  
Author(s):  
P. Duvdevani ◽  
N. Varsano ◽  
R. Slepon ◽  
Y. Lerman ◽  
T. Shohat ◽  
...  
Keyword(s):  
Young Adults ◽  
Enzyme Immunoassay ◽  
Hemagglutination Inhibition ◽  
Comparative Evaluation ◽  
Measles Virus ◽  
Commercial Enzyme ◽  
Commercial Enzyme Immunoassay

scholarly journals Determination of salivary cortisol in donkey stallions

2018 ◽  
Author(s):  
MICAELA SGORBINI ◽  
Francesca Bonelli ◽  
Alessandra Rota ◽  
Christine Aurich ◽  
Natascha Ille ◽  
...  
Keyword(s):  
Enzyme Immunoassay ◽  
Salivary Cortisol ◽  
Circadian Variation ◽  
Cross Reactivity ◽  
Commercial Enzyme ◽  
Standard Curve ◽  
Non Invasive ◽  
Detectable Concentration ◽  
Commercial Enzyme Immunoassay

Salivary cortisol provides information about free plasma cortisol concentration and salivary sampling is a non-invasive well-tolerate procedure. The aim of this study was to validate a commercial enzyme immunoassay for the determination of salivary cortisol in donkeys. Saliva samples were collected in 4 donkey stallions on thirteen non-consecutive days at 8:30 AM to avoid circadian variation. Animals were already accustomed to be handled. Saliva was collected by using a swab inserted at the angle of the lips, placed onto the tongue for 1 min and returned into a polypropylene tube. Tubes were centrifuged and at least 1 ml of saliva was aspirated from each sample and frozen at −20° C until analysis. A commercial enzyme immunoassay kit without extraction was used for determination of cortisol in saliva. Median cortisol concentrations with minimum and maximum value were calculated. Recovery of cortisol standard in donkey saliva was 107.9% and serial dilution of donkey saliva samples with assay buffer resulted in changes in optical density parallel to the standard curve. Cross-reactivity of the antiserum was 10.4% with 11-deoxycortisol, 5.2% with corticosterone, 0.4% with 11-deoxycorticosterone, 0.2% with cortisone and <0.1% with testosterone, progesterone and estradiol. The intra-assay coefficient of variation was 10.7%, the inter-assay variation was 8.0% and the minimal detectable concentration was 0.01 ng/ml. The results of the present study demonstrate the validity of a commercial kit to determine the concentration of cortisol in donkey saliva, as already reported in other species.

Evaluation of a commercial enzyme immunoassay for HIV screening in urine

2004 ◽  
Vol 23 (11) ◽  
pp. 831-835 ◽  
Author(s):  
J. Almeda ◽  
J. Casabona ◽  
L. Matas ◽  
V. Gonz�lez ◽  
R. Muga ◽  
...  
Keyword(s):  
Enzyme Immunoassay ◽  
Hiv Screening ◽  
Commercial Enzyme ◽  
Commercial Enzyme Immunoassay
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