scholarly journals Determination of salivary cortisol in donkey stallions

2018 ◽  
Author(s):  
MICAELA SGORBINI ◽  
Francesca Bonelli ◽  
Alessandra Rota ◽  
Christine Aurich ◽  
Natascha Ille ◽  
...  
Keyword(s):  
Enzyme Immunoassay ◽  
Salivary Cortisol ◽  
Circadian Variation ◽  
Cross Reactivity ◽  
Commercial Enzyme ◽  
Standard Curve ◽  
Non Invasive ◽  
Detectable Concentration ◽  
Commercial Enzyme Immunoassay

Salivary cortisol provides information about free plasma cortisol concentration and salivary sampling is a non-invasive well-tolerate procedure. The aim of this study was to validate a commercial enzyme immunoassay for the determination of salivary cortisol in donkeys. Saliva samples were collected in 4 donkey stallions on thirteen non-consecutive days at 8:30 AM to avoid circadian variation. Animals were already accustomed to be handled. Saliva was collected by using a swab inserted at the angle of the lips, placed onto the tongue for 1 min and returned into a polypropylene tube. Tubes were centrifuged and at least 1 ml of saliva was aspirated from each sample and frozen at −20° C until analysis. A commercial enzyme immunoassay kit without extraction was used for determination of cortisol in saliva. Median cortisol concentrations with minimum and maximum value were calculated. Recovery of cortisol standard in donkey saliva was 107.9% and serial dilution of donkey saliva samples with assay buffer resulted in changes in optical density parallel to the standard curve. Cross-reactivity of the antiserum was 10.4% with 11-deoxycortisol, 5.2% with corticosterone, 0.4% with 11-deoxycorticosterone, 0.2% with cortisone and <0.1% with testosterone, progesterone and estradiol. The intra-assay coefficient of variation was 10.7%, the inter-assay variation was 8.0% and the minimal detectable concentration was 0.01 ng/ml. The results of the present study demonstrate the validity of a commercial kit to determine the concentration of cortisol in donkey saliva, as already reported in other species.

Evaluation of a solid-phase enzyme immunoassay for human choriogonadotropin beta subunit.

1983 ◽  
Vol 29 (11) ◽  
pp. 1964-1966 ◽  
Author(s):  
D D Davey ◽  
M M Sample ◽  
T O Oei
Keyword(s):  
Enzyme Immunoassay ◽  
Solid Phase ◽  
Cross Reactivity ◽  
Beta Subunit ◽  
Neoplastic Disease ◽  
Standard Curve ◽  
Human Choriogonadotropin ◽  
Beta Hcg ◽  
Substantial Problem ◽  
Sample Dilution

Abstract We evaluated a quantitative solid-phase enzyme immunoassay for human choriogonadotropin beta subunit (beta-HCG) with anti-beta-HCG:horseradish peroxidase conjugate, recently marketed by Abbott Laboratories. We compared results on 56 patients' serum specimens, obtained mostly for followup of neoplastic disease, with those by a competitive radioimmunoassay kit. The correlation was good, the differences being of little clinical significance. Linear regression in the low and intermediate ranges gave a slope of 0.93, a y-intercept of 0.34, and a correlation coefficient of 0.97. Precision studies yielded an interassay CV of 6.4% in the intermediate range and 13% in the low range. Sensitivity was 0.69 int. unit/L. Cross reactivity was 1 to 2% with specimens fortified with lutropin or follitropin. The only substantial problem was with linearity in the upper part of the standard curve, especially in the interval, 100-200 int. units/L. This problem is obviated by adequate sample dilution.

Development and Characterization of Anti-Estradiol Polyclonal Antibody

2012 ◽  
Vol 461 ◽  
pp. 67-70 ◽  
Author(s):  
Chao Ying Li ◽  
Jin Qing Jiang
Keyword(s):  
Polyclonal Antibody ◽  
Dynamic Range ◽  
Cross Reactivity ◽  
Enzyme Linked Immunosorbent Assay ◽  
Uv Absorbance ◽  
Standard Curve ◽  
Ic50 Value ◽  
New Zealand White Rabbits

This paper reports an indirect competitive enzyme-linked immunosorbent assay (icELISA) using polyclonal antibody (pAb) for estradiol (E2) residues. After derivation, E2 haptens were conjugated to bovine serum albumin (BSA) and ovalbumin (OVA) through 1-Ethyl-3-(3-dimethylaminopropy) carbodiimide (EDC) method, and New Zealand white rabbits were immunized to produce anti-E2 pAb. The conjugation ratio of E2-BSA was proved to be 18.6:1 by an UV absorbance method. Based on the square matrix titration, an icELISA standard curve was developed. The dynamic range was from 0.16 to 128 ng/mL, with LOD and IC50 value of 0.08 ng/mL and 3.76 ng/mL, respectively. Except for a little cross-reactivity (16.2%) to estrone, this assay showed negligible cross-reactivity to other analogues tested. The results suggest that the produced anti-E2 pAb could be used to develop an icELISA method for the determination of E2 residues in animal-originally products.

Comparison of a new commercial enzyme immunoassay for rapid detection of respiratory syncytial virus

1992 ◽  
Vol 11 (2) ◽  
pp. 175-177 ◽  
Author(s):  
M. T. Garea ◽  
J. M. Lopez ◽  
M. L. Perez del Molino ◽  
A. Coira ◽  
F. Pardo
Keyword(s):  
Respiratory Syncytial Virus ◽  
Enzyme Immunoassay ◽  
Rapid Detection ◽  
Commercial Enzyme ◽  
Syncytial Virus ◽  
Commercial Enzyme Immunoassay

scholarly journals Development of enzyme immunoassay for endogenous ouabain-like compound in human plasma

1997 ◽  
Vol 43 (5) ◽  
pp. 715-722 ◽  
Author(s):  
Steven Harwood ◽  
John A Little ◽  
Gerard Gallacher ◽  
David Perrett ◽  
Raymond Edwards ◽  
...  
Keyword(s):  
Detection Limit ◽  
Healthy Volunteers ◽  
Enzyme Immunoassay ◽  
Human Plasma ◽  
Hplc Analysis ◽  
Cross Reactivity ◽  
Standard Curve ◽  
Endogenous Ouabain ◽  
Endogenous Steroid ◽  
Hplc Study

Abstract Widespread evidence supports the existence of an endogenous digitalis-like compound in mammals. We report here the development of a novel enzyme immunoassay for ouabain that, in conjunction with a detailed HPLC study, identifies a ouabain-like compound (OLC) in extracted human plasma. The assay is sensitive—minimum detection limit for OLC 37 pmol/L (11 pmol/L in plasma)—and has a working range (between-assay CV &lt;10%) of 180–10 000 pmol/L (54–3000 pmol/L in plasma). Mean recoveries of ouabain added to plasma ranged from 90% to 100%, and plasma extracts diluted in parallel to the standard curve. Plasma OLC concentrations in 10 healthy volunteers averaged 92 pmol/L (range 55–168), assuming 100% cross-reactivity of OLC in the ouabain assay. HPLC analysis with two distinct chromatographic conditions demonstrated that endogenous human plasma OLC co-eluted with authentic ouabain. The enzyme immunoassay is rapid and easy to perform and will support further investigation of the nature of this controversial endogenous steroid.

scholarly journals Commercial enzyme immunoassay reagent pack for ouabain compared with human placenta radioreceptor assay

1996 ◽  
Vol 42 (4) ◽  
pp. 648-650 ◽  
Author(s):  
A Paci ◽  
A Ledda ◽  
G Ciarimboli ◽  
P Biver ◽  
G P Bernini
Keyword(s):  
Enzyme Immunoassay ◽  
Human Placenta ◽  
Radioreceptor Assay ◽  
Commercial Enzyme ◽  
Commercial Enzyme Immunoassay

Evaluation of a commercial enzyme immunoassay for detection ofMycoplasma pneumoniae specific immunoglobulin G antibodies

1990 ◽  
Vol 9 (3) ◽  
pp. 221-223 ◽  
Author(s):  
S. A. Uldum ◽  
J. Søndergård-Andersen ◽  
J. Skov Jensen ◽  
K. Lind
Keyword(s):  
Enzyme Immunoassay ◽  
Immunoglobulin G ◽  
Commercial Enzyme ◽  
Specific Immunoglobulin ◽  
Commercial Enzyme Immunoassay

Evaluation of a commercial enzyme immunoassay for HIV screening in urine

2004 ◽  
Vol 23 (11) ◽  
pp. 831-835 ◽  
Author(s):  
J. Almeda ◽  
J. Casabona ◽  
L. Matas ◽  
V. Gonz�lez ◽  
R. Muga ◽  
...  
Keyword(s):  
Enzyme Immunoassay ◽  
Hiv Screening ◽  
Commercial Enzyme ◽  
Commercial Enzyme Immunoassay
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