Comparison of progesterone concentration determination by 12 non-isotopic immunoassays and gas chromatography/mass spectrometry in 99 human serum samples

2001 ◽  
Vol 78 (1) ◽  
pp. 97-104 ◽  
Author(s):  
Philippe Boudou ◽  
Joëlle Taieb ◽  
Bruno Mathian ◽  
Yves Badonnel ◽  
Isabelle Lacroix ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Human Serum ◽  
Gas Chromatography Mass Spectrometry ◽  
Progesterone Concentration ◽  
Serum Samples ◽  
Human Serum Samples ◽  
Concentration Determination ◽  
Chromatography Mass Spectrometry

scholarly journals Determination of Mammalian Lignans in Biological Samples by Modified Gas Chromatography/Mass Spectrometry

2007 ◽  
Vol 90 (3) ◽  
pp. 641-646 ◽  
Author(s):  
Ajay Bommareddy ◽  
Bhanu L Arasada ◽  
Duane P Mathees ◽  
Chandradhar Dwivedi
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Gas Chromatography Mass Spectrometry ◽  
Serum Samples ◽  
Selected Ion Monitoring ◽  
Beneficial Effects ◽  
Chromatography Mass Spectrometry ◽  
Flaxseed Meal

Abstract Lignans in flaxseed have been part of the human diet for centuries. In 1955, the isolation and structure of the lignan derivative secoisolariciresinol diglucoside (SDG) was reported. The biological role of SDG and mammalian lignan metabolites enterodiol and enterolactone was initially reported 20 years later. Experimental evidences showed the beneficial effects of lignans on breast, colon, and thyroid cancer. A modified gas chromatography/mass spectrometry (GC/MS) assay was developed for lignans in serum and colon samples of rats fed flaxseed meal. The method developed for the analysis of metabolites involves extraction and derivatization of samples and quantitative analysis by selected ion monitoring using GC/MS. The levels of lignan metabolites enterodiol and enterolactone were determined to be 0.013 and 0.23 M in serum samples and 0.008 and 1.63 M in colon samples.

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