ESTRATÉGIAS NUTRICIONAIS PARA AUMENTAR AS PROPRIEDADES NUTRACÊUTICAS DO LEITE: CONCENTRAÇÕES DE ENTEROLACTONA E ÁCIDO LINOLEICO CONJUGADO

Objetivou-se descrever estratégias nutricionais para aumentar as propriedades nutracêuticas do leite, incluindo principalmente estratégias para o aumento nas concentrações de enterolactona (EL) e ácido linoleico conjugado (CLA). Os compostos EL e CLA, ambos produzidos endogenamente a partir de componentes da dieta, são compostos bioativos presente no leite e têm sido apontados como compostos nutracêuticos, capazes de melhorar a saúde humana através da prevenção e tratamento de doenças. Estratégias nutricionais aplicadas na alimentação de vacas leiteiras possibilitam o aumento da concentração destes compostos no leite, tornando possível a produção de leite com maior potencial de beneficiamento a saúde humana. A inclusão de fontes de lignanas vegetais, como por exemplo, o farelo de linhaça e a inclusão de fontes de sacarose na dieta de vacas leiteiras possibilitam aumentar a concentração de EL no leite. Da mesma forma, o fornecimento de fontes ricas de ácidos graxos polinsaturados, como por exemplo, os óleos vegetais, possibilita o aumento da concentração de CLA no leite. A temática da nutrição animal voltada para o aumento das propriedades nutracêuticas do leite, apesar de estar iniciando, apresenta grande perspectiva, e é uma ferramenta valiosa para promoção da associação do leite com benefícios a saúde humana. Palavras-chave: nutracêutico; compostos bioativos; lignanas; vacas de leite; benefícios do leite; redução do risco de doenças. Nutritional strategies to improve nutraceutical proprieties of milk: improvements on milk enterolactone and conjugated linoleic acid ABSTRACT: This study aimed to describe nutritional strategies to increase milk nutraceutical properties, including mainly strategies to increase the concentrations of enterolactone (EL) and conjugated linoleic acid (CLA). Enterolactone and CLA are endogenously produced from dietary components and have been identified as nutraceutical compounds with the potential of improving human health through the prevention and treatment of diseases. The concentration of these compounds in milk can be modulated through nutritional strategies which enable to produce milk with greater potential for improving human health. The inclusion of vegetable lignans sources, such as flaxseed meal and the inclusion of sucrose sources in the diet of dairy cows increase the concentration of EL in milk. Feeding sources of polyunsaturated fatty acids, such as vegetable oils, results in increased concentration of CLA in milk. The application of animal nutrition to increase nutraceutical properties of milk is a valuable tool for promoting the association of milk with human health benefits and is of great interest. Keywords: nutraceutical proprieties; bioactive compounds; lignans; dairy cows; health benefits of milk; disease risk reduction.

Enriching laying hens eggs by feeding diets with different fatty acid composition and antioxidants

The current study was conducted to evaluate egg quality, egg yolk fatty acids, health-related indices and antioxidants from laying hens' eggs fed different combined vegetable by-products, rich in fatty acids and antioxidants. One hundred twenty 50 weeks-old Tetra SL laying hens were divided into three groups. They were given daily a standard diet (Control, C), a diet containing 9% rapeseed meal with 3% grapeseed meal (T1 diet), or a diet containing 9% flaxseed meal and 3% sea buckthorn meal (T2 diet). Hen production performances, egg quality, egg yolk fatty acids total polyphenols content and antioxidant capacity were determined. The T1 diet significantly reduced the egg yolk content of palmitic acid from 76.615 mg (C) to 46.843 mg (T1) and that of oleic acid from 788.13 mg (C) to 682.83 mg (T1). Feeding flaxseed and sea buckthorn meals significantly increased the egg yolk content of α-linolenic acid in T2 yolks (35.297 mg) compared with C yolks (4.752 mg) and that of docosahexaenoic acid (DHA) from 16.282 mg (C) to 74.918 mg (T2). The atherogenicity indices (AI) were not significantly affected, whereas the thrombogenicity indices (TI) decreased significantly (p < 0.0007) from 0.72 (C) to 0.60 (T1) and 0.66 (T2), respectively. Adding this combination of meals to the hens' diets, increased the total polyphenol content and antioxidant capacity in T1 and T2 eggs compared to C eggs. The significant enrichment of eggs with n-3 fatty acids and antioxidant capacity, as well on the health-related indices especially from T2 eggs, represents a potential functional feed ingredient in poultry feeding, to obtain eggs as functional food.

Ultrasonic-Assisted Aqueous Two-Phase Extraction Combined with Macroporous Resin Enrichment of Lignans from Flaxseed Meal and Their Antioxidant Activities

Background Secoisolariciresinol di glucoside (SDG) is a natural antioxidant generally extracted from flaxseed, which is one of the most important oil crops in China, the by-product of the flaxseed oil, i.e., flaxseed meal, still contains a lot of lignans. However, flaxseed meal is generally treated as waste, resulting in a huge waste of resources. Objective To establish an efficient and convenient method for extraction and purification of lignans from flaxseed meal. Methods First, we used response surface methodology (RSM) to optimize the extraction conditions of the ultrasonic-assisted aqueous two-phase system (UAATPS), and we obtained the purified extracts by macroporous resin purification (MRP). Second, the antioxidant ability of the extracts was studied in vitro. Results The best extraction conditions obtained were as follows: 9.0 wt% sodium hydroxide, 30.0 wt% isopropanol, extraction time 39 min, liquid-to-solid ratio of 52.0 mL/g, ultrasonic wave 560 W, and extraction temperature 40 °C. Under the optimal conditions, the purity of crude extracts (SDG-APTS-C) reached 21.5%. The desorption conditions of MRP were as follows: eluting 3 BV with ultrapure water, and then eluting with 25% ethanol at 2 BV/h to collect eluents. The purified extracts (SDG-ATPS-P) had a purity quotient of 73.9%, 52.4% higher than that of SDG-ATPS-C. Additionally, experiments conducted in this paper revealed that SDG-ATPS-C and SDG-ATPS-P could effectively remove DPPH, ABTS, and hydroxyl free radicals in vitro. Conclusions The method was validated for extracting SDG from flaxseed meal, thus achieving the reuse of flaxseed meal. Highlights This research provides some references for the application of UAATPS combined with MRP in natural products.

Apparent and standardised digestibility in broilers fed flaxseed meal with multicarbohydrase and phytase at 35 days of age

The apparent total tract digestibility (ATTD; trial 1) and the apparent (AID) and standardized (SID) ileal digestibility of the amino acids (AA) (trial 2) in flaxseed meal (FM) were evaluated with the addition of exogenous multi-carbohydrase (MC) and phytase (Phy). A total of 80 28-day-old broilers were allotted in a completely randomized design to receive treatments up to 35 days of age. A 2 × 2 factorial design as used to determine the enzyme effects, on the ATTD of dry matter, nitrogen, calcium, phosphorus and fibre; energy use and the AID and SID of AA, in five replicate cages. There was synergism between MC and Phy on the ATTD of nitrogen and energy. The isolated inclusion of MC and Phy on the ATTD of dry matter, calcium, phosphorus and fibre of FM. The enzyme inclusion on the AID and SID of AA in FM, established by comparing the means, suggested a better response to the addition of MC + Phy. Supplementation with Phy or combination of MC and Phy was a viable alternative to increase the ATTD of nutrients and energy. The Combination of MC + Phy resulted in higher apparent and standardised digestibility of amino acidsAA from FM.

FLAXSEED MEAL FEEDING TO DAIRY COWS AS A STRATEGY TO IMPROVE MILK ENTEROLACTONE CONCENTRATION: A LITERATURE REVIEW

ABSTRACT: Flaxseed (Linum usitatissimum) is the richest source of the plant lignan secoisolariciresinol diglucoside (SDG). In mammals, including bovine, SDG is converted to the mammalian lignans enterolactone (EL) and enterodiol (ED) by the action of gastrointestinal microbes. There is a great deal of interest in promoting increased intakes of lignans in humans’ diet due to the potential health benefits of mammalian lignans, especially in the prevention of cardiovascular diseases, hypercholesterolaemia, breast and prostate cancers, and osteoporosis. Consumption of milk and dairy products enriched in EL could be an excellent strategy to increase the intake of lignans by humans. This literature review will focus on presenting feeding strategies capable to improve milk enterolactone concentration. Research has demonstrated the potential of flaxseed meal (FM) feeding to dairy cows as a strategy to improve milk EL concentration, therefore enhancing milk nutraceutical proprieties. A considerable number of studies have demonstrated that feeding vegetable lignans-rich sources, such as FM, to dairy cows improves EL in milk. Additionally, it has been reported that changes in the carbohydrate profile of FM-based diets fed to dairy cows can alter the output of milk EL. The application of animal nutrition as a tool to increase nutraceutical properties of milk (i.e. increased EL concentration) is a valuable strategy for promoting the association of milk with humans’ health benefits and is of great interest in contemporary society. Keywords: nutraceutical proprieties, bioactive compounds, lignans, dairy cows, disease risk reduction

Process Parameter Optimization and Characterization for an Edible Film: Flaxseed Concern

Consumer demands for biocompatible, minimally processed and eco-friendly foods have increased drastically and are currently trending. Polysaccharides derived from various plant seeds exhibiting structure conformational diversity are among such foods and used for the development of edible films. In this study, the physical properties of flaxseed, proximate characterization and rheological, mechanical and thermal features of flaxseed meal-based edible films were investigated. A development strategy worked through adding pectin + flaxseed meal to a plasticizer (glycerol) with a ratio of 7:3 w/v, whereas, in the control group, the flaxseed meal remained unaltered. The rheological results showed the non-Newtonian behavior of film-forming solutions and data were well fitted into the power law model. The developed film (flaxseed + pectin) was slightly brownish and exhibited a transparency of 17.78, which is clear enough to be used as see-through packaging material, whereas the control sample had a transparency of 38.25, indicating its fair transparency. The water vapor permeability of the test sample was also beneficial (0.992 g/cm2/24 h) and was competitively close to the control (0.981 g/cm2/24 h). The developed blended films were 98–99% soluble in water and acid, indicating their usefulness when applied as a coating. The mechanical properties, tensile strength and elongation value of the test sample were less than the control. This study will be helpful in the development of a novel biodegradable film for extending the life of different foods.

Influence of different concentrations of chia (Salvia hispanica) and flaxseed (Linum usitatissimum) meal on fillet fatty acid profile of Nile tilapia (Oreochromis niloticus)

The nutritional quality of tilapia fillet is characterized by low omega-3 polyunsaturated fatty acids (n-3 PUFAs). The objective of the present study was to evaluate the effect of different dietary concentrations of chia (Salvia hispanica) and flaxseed (Linum usitatissimum) meal on the omega-3 fatty acid content of tilapia (Oreochromis niloticus) fillet. Six experimental diets incorporating 5, 10, and 15% chia or flaxseed meals, respectively, were given to the fish (238.36 ± 7.61 g, initial weight) for 45 days before harvesting. The α-linolenic acid (ALA) and n-3 PUFA contents in the fillet differed significantly among diets (P < 0.05). Diets containing 15% chia or flaxseed meal had the highest concentrations of ALA (4.80 and 4.75%, respectively) and n-3 PUFA (13.65 and 15.92%, respectively). Thus, chia or flaxseed meal dietary inclusion increased the ALA and n-3 PUFA contents in the tilapia fillet. Chia or flaxseed meals are useful and potential alternatives to improve the nutritional value of O. niloticus fillets.

Impact of Dietary Supplementation of Flaxseed Meal on Intestinal Morphology, Specific Enzymatic Activity, and Cecal Microbiome in Broiler Chickens

The use of natural feed additives could be a beneficial approach to maintaining the health of chickens and a way to improve food digestion. Flaxseed is a rich source of omega-3 fatty acid, alpha linolenic acid, oleic acid, and fiber. The purpose of this study was to evaluate the effects of dietary inclusion of 4% flaxseed on the intestinal morphology, specific enzymatic activity, and cecal microbiome in broiler chickens. The 4-week feeding trial was conducted on 100 Cobb 500 (14 days of age) unsexed broiler chickens divided into two groups: a control group (C) and an experimental group (E). The broilers were housed in boxes of size 3 m2 (each group was housed in a single box with 10 replicates, 5 chickens per replicate) and reared on permanent wood shaves litter (10–12 cm thick). At the end of the experiment, chickens (n = 10) were sacrificed and tissue samples were harvested from the duodenum, jejunum, and cecum for histological, enzymatic, and microbiome analyses. In group E, histological analysis revealed a significant increase in villus height (p < 0.001) possibly leading to enhanced intestinal nutrient absorption. An increase in the specific activities of α-amylase (p < 0.05), invertase (p < 0.01), and endo-β-1,4-glucanase (p < 0.001) was noticed in the E group for the duodenum and jejunum compared to the control group. In contrast, maltase activity decreased in the duodenum and increased in the jejunum in the E group. The trypsin and lipase specific activities did not vary in a significant way. In addition, the cecal microbiome of the E group was characterized by an increase in Lactobacilli (p < 0.01) and Clostridium coccoides and a decrease in Bacteroides, Ruminoccocus, Enterobacteriaceae, and Clostridium leptum. In conclusion, our results suggest that dietary supplementation of flaxseed meal may boost intestinal health status in poultry.

Bioethanol Production from Enzymatic Hydrolyzates of Pretreated Flaxseed Meals by Baker’s Yeast

The present study investigated the usefulness of flaxseed meals as a novel feedstock for the production of bioethanol. The proximate composition of the flaxseed meal was carried out before the pretreatment of the flaxseed meal. In this study, flaxseed meal was pretreated with dilute acid, alkali, and aqueous for disruption of lignocellulosic compounds. The acid pretreated flaxseed meal was used for enzymatic hydrolysis by different enzymes (cellulase, α-amylase, and cellulase combined with α-amylase) for better release of reducing sugar. The cellulose conversion to reducing sugar was significantly higher for acid pretreated flaxseed meals. After enzymatic hydrolysis with cellulase, cellulose conversions to reducing sugars were found to be significantly higher than those of α-amylase and cellulase combined with α-amylase. The bioethanol production was also investigated. The fermentation process was carried out by using baker’s yeast (Saccharomyces cerevisiae) with the acid pretreated flaxseed meal enzymatic hydrolyzate. Maximum ethanol production (0.11 g/l) was achieved from the fermented medium obtained from the acid pretreated flaxseed meal followed by enzymatic hydrolysis by using cellulase enzyme. The structural analysis of bioethanol was also investigated by FTIR.