Interaction between estradiol and growth factors in the regulation of specific gene expression in MCF-7 human breast cancer cells

1997 ◽  
Vol 60 (5-6) ◽  
pp. 269-276 ◽  
Author(s):  
Mohammed K.K. El-Tanani ◽  
Chris D. Green
Keyword(s):  
Breast Cancer ◽  
Gene Expression ◽  
Growth Factors ◽  
Cancer Cells ◽  
Human Breast Cancer ◽  
Breast Cancer Cells ◽  
Specific Gene ◽  
Human Breast Cancer Cells ◽  
Human Breast ◽  
Mcf 7

scholarly journals Melatonin enhances the apoptotic effects and modulates the changes in gene expression induced by docetaxel in MCF‑7 human breast cancer cells

2017 ◽  
Author(s):  
Carolina Alonso-Gonz�lez ◽  
Javier Men�ndez-Men�ndez ◽  
Alicia Gonz�lez-Gonz�lez ◽  
Alicia Gonz�lez ◽  
Samuel Cos ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Gene Expression ◽  
Cancer Cells ◽  
Human Breast Cancer ◽  
Breast Cancer Cells ◽  
Human Breast Cancer Cells ◽  
Human Breast ◽  
Apoptotic Effects ◽  
Mcf 7

scholarly journals Estrogen regulation of transferrin gene expression in MCF-7 human breast cancer cells

2002 ◽  
Vol 29 (3) ◽  
pp. 305-317 ◽  
Author(s):  
C Vyhlidal ◽  
X Li ◽  
S Safe
Keyword(s):  
Breast Cancer ◽  
Gene Expression ◽  
Cancer Cells ◽  
Human Breast Cancer ◽  
Breast Cancer Cells ◽  
Human Breast Cancer Cells ◽  
Human Breast ◽  
Estrogen Regulation ◽  
Tf Gene ◽  
Mcf 7

Transferrin (Tf) is an iron transport protein expressed in MCF-7 human breast cancer cells. In nuclear run-on assays, 17beta-estradiol (E2) increased the rate of Tf gene expression approximately 3-fold within 1 h after treatment and reporter gene activity was also induced in MCF-7 cells transfected with a construct containing a -3600 to +39 Tf gene promoter insert. Deletion and mutation analysis identified an E2-responsive promoter region between -811 and -762, which was GC-rich (80%) and contained two nonconsensus estrogen response elements (EREs). E2-responsiveness of this region was associated with a GGACA(N)(3)TGGCC motif (-803 to -791) which bound human estrogen receptor alpha (hERalpha) in gel mobility shift assays. In Drosophila Schneider SL-2 cells, the -811 to -752 was E2-responsive after cotransfection with hERalpha expression plasmid plus E2, whereas Sp1 protein did not induce transactivation. These studies confirm that E2 induces Tf gene expression through a nonconsensus distal ERE.

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