scholarly journals Matrix effect in bio-analysis of illicit drugs with LC-MS/MS: Influence of ionization type, sample preparation, and biofluid

2003 ◽  
Vol 14 (11) ◽  
pp. 1290-1294 ◽  
Author(s):  
Riet Dams ◽  
Marilyn A. Huestis ◽  
Willy E. Lambert ◽  
Constance M. Murphy
Keyword(s):  
Sample Preparation ◽  
Matrix Effect ◽  
Illicit Drugs ◽  
Type Sample

A Modified QuEChERS Extraction and LC-MS/MS Method for the Determination of Pesticide Residues in Curry Leaves (Murraya koenigii)

2019 ◽  
Vol 6 (1) ◽  
pp. 30-41
Author(s):  
Ranjith Arimboor ◽  
Karunkara Ramakrishna Menon ◽  
Natarajan Ramesh Babu ◽  
Haneesh Chandran
Keyword(s):  
Sample Preparation ◽  
Matrix Effect ◽  
Retention Time ◽  
Pesticide Residues ◽  
Mobile Phase ◽  
Preparation Technique ◽  
Modified Quechers ◽  
Curry Leaves ◽  
Ms Analysis ◽  
The Matrix

Background:Increased consumer demand for curry leaves free from pesticides demands fast and reliable analytical methods for the analysis of pesticide residues.Objective:The optimization of a QuEChERS based sample preparation technique with improved analytical accuracy by removing interfering matrix components for LC-MS/MS analysis of pesticide residues from curry leaves.Methods:A modified QuEChERS solid phase extraction method was developed and validated for the analysis of 26 pesticides in fresh and dried curry leaves. The effects of the sample preparation steps and column retention time on the matrix suppression of analyte ions were also evaluated.Results:Validation parameters were found within an acceptable range. The matrix effect evaluation studies showed that the QuEChERS sample preparation was able to minimize the ion suppression of analytes due to co-eluting matrix of components and that a d-SPE clean up step had major role in reducing matrix effect. The gradient mobile phase with longer retention time for analytes resulted in comparatively lesser matrix effects than the isocratic mobile phase of non-polar nature. Even after the clean up, a considerable number of compounds had more than 20% reduction in their MS response in the gradient mobile phase.Conclusion:This study emphasized the need of proper sample clean up before a LC-MS/MS analysis and the usage of matrix matched standards and mobile phase that ultimately results in an appropriate analyte separation in reasonable retention times.

Evaluation of Effects on Tritium Measurement According to HTO Type Sample Preparation Conditions

2021 ◽  
Vol 44 (4) ◽  
pp. 381-387
Author(s):  
Eun-Mi An ◽  
◽  
Jung-Hoon Kim ◽  
Hong-Yeon Lee ◽  
Sang-Jun Han ◽  
...  
Keyword(s):  
Sample Preparation ◽  
Preparation Conditions ◽  
Type Sample

Fiber-packed needle-type sample preparation device designed for gas chromatographic analysis

2008 ◽  
Vol 393 (3) ◽  
pp. 861-869 ◽  
Author(s):  
Yoshihiro Saito ◽  
Ikuo Ueta ◽  
Mitsuhiro Ogawa ◽  
Akira Abe ◽  
Kentaro Yogo ◽  
...  
Keyword(s):  
Sample Preparation ◽  
Chromatographic Analysis ◽  
Needle Type ◽  
Gas Chromatographic Analysis ◽  
Type Sample

Novel Multiresidue Method for the Determination of Eight Trichothecene Mycotoxins in Pollen Samples Using QuEChERS-Based GC-MS/MS

2017 ◽  
Vol 68 (2) ◽  
pp. 304-306
Author(s):  
Ionela Daniela Morariu ◽  
Liliana Avasilcai ◽  
Madalina Vieriu ◽  
Alina Diana Panainte ◽  
Nela Bibire
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Sample Preparation ◽  
Matrix Effect ◽  
Limit Of Quantification ◽  
Multiresidue Method ◽  
Good Linearity

A simple multi-residue method based on QuEChERS-sample preparation was developed for the determination of eight trichothecene mycotoxins (nivalenol, fusarenon-x, diacetoxyscirpenol, 3-acetyl-deoxynivalenol, neosolaniol, deoxynivalenol, T-2 and HT-2 toxins) in pollen, using gas chromatography coupled with mass spectrometry. The developed method was validated by evaluating the following parameters: linearity, limit of quantification, matrix effect and precision of measurements. All mycotoxins showed good linearity. The method gave good recoveries (91-104%) with RSDs ] 11% for most of the analites (6 out of 8). The limits of quantification varied from 1 to 4�g�kg--1.

scholarly journals Development and validation of a LC-ESI-MS/MS method for simultaneous whole blood analysis of 51 new psychoactive substances

2019 ◽  
Vol 3 (2) ◽  
pp. 36-45 ◽  
Author(s):  
Maria Cristina Franck ◽  
Maristela Goldnadel Monteiro ◽  
Renata Pereira Limberger
Keyword(s):  
Matrix Effect ◽  
Whole Blood ◽  
Illicit Drugs ◽  
Limit Of Detection ◽  
Synthetic Cannabinoids ◽  
Forensic Analysis ◽  
Blood Analysis ◽  
New Psychoactive Substances ◽  
Esi Ms ◽  
Validation Parameters

In recent years, there has been a great increase in seizures and forensic analysis of new psychotropic substances (NPS) in the state of Rio Grande do Sul. The analysis of these compounds needs to be performed in biological samples in cases of violent deaths. A sensitive and reliable liquid chromatography-tandem mass spectrometry with electrospray ionization interface (LC-ESI-MS/MS) method was developed and validated for qualitative analysis of 51 NPS in whole blood forensic samples. Synthetic cathinones, phenethylamines, opioids, tryptamines, synthetic cannabinoids, and other hallucinogens and stimulants were included in the method. The validation parameters assessed were specificity, limit of detection, retention time precision, and matrix effect. Drug free pools (n=6) were used for validation, including post mortem samples as well as from living individuals. Adulterants, pharmaceuticals, metabolites, and other illicit drugs, totalling 39 compounds, were analyzed and no interference was noticed. The detection limits obtained were suitable for evaluation at recreational and non-fatal levels of consumption, mostly. The results revealed an appropriate matrix effect in 24 out of 51 substances tested, indicating the potential for future quantitative analysis with this method for these drugs. The developed and validated method is easy to implement, fast, with low cost, and suitable for use in routine forensic toxicology laboratory analysis.

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