Endothelial cell seeding of vascular grafts: Experimental investigations and clinical update

1996 ◽  
Vol 5 (5) ◽  
pp. 296 ◽  
Author(s):  
Manfred Deutsch ◽  
Johann Meinhart ◽  
Teddy Fischlein ◽  
Peter Zilla
Keyword(s):  
Endothelial Cell ◽  
Vascular Grafts ◽  
Experimental Investigations ◽  
Cell Seeding ◽  
Endothelial Cell Seeding

Microvascular Endothelial Cell Seeding of Small-Diameter Dacron|2TM|0 Vascular Grafts

1988 ◽  
Vol 1 (1) ◽  
pp. 35-44 ◽  
Author(s):  
Steven P. Schmidt ◽  
Navid Monajjem ◽  
M. Michelle Evancho ◽  
Todd R. Pippert ◽  
W. V. Sharp
Keyword(s):  
Endothelial Cell ◽  
Vascular Grafts ◽  
Small Diameter ◽  
Microvascular Endothelial Cell ◽  
Cell Seeding ◽  
Endothelial Cell Seeding ◽  
Microvascular Endothelial

Fibrinolytic profiles after endothelial cell seeding of prosthetic vascular grafts

1994 ◽  
Vol 8 ◽  
pp. 139
Author(s):  
J.V. Smyth ◽  
M. Welch ◽  
H.M.H. Carr ◽  
P. Eisenberg ◽  
M.G. Walker
Keyword(s):  
Endothelial Cell ◽  
Vascular Grafts ◽  
Cell Seeding ◽  
Endothelial Cell Seeding

Endothelial Cell Seeding on Crosslinked Collagen: Effects of Crosslinking on Endothelial Cell Proliferation and Functional Parameters

2000 ◽  
Vol 84 (08) ◽  
pp. 325-331 ◽  
Author(s):  
M. J. B. Wissink ◽  
M. J. A. van Luyn ◽  
R. Beernink ◽  
F. Dijk ◽  
A. A. Poot ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Endothelial Cell ◽  
Plasminogen Activator ◽  
Type I Collagen ◽  
Vascular Grafts ◽  
Small Diameter ◽  
Endothelial Cell Proliferation ◽  
Type I ◽  
Cell Seeding ◽  
Endothelial Cell Seeding

SummaryEndothelial cell seeding, a promising method to improve the performance of small-diameter vascular grafts, requires a suitable substrate, such as crosslinked collagen. Commonly used crosslinking agents such as glutaraldehyde and formaldehyde cause, however, cytotoxic reactions and thereby hamper endothelialization of currently available collagen-coated vascular graft materials.The aim of this study was to investigate the effects of an alternative method for crosslinking of collagen, using N-(3-dimethylaminopropyl)-N’-ethylcarbodiimide (EDC) in combination with N-hydroxysuccinimide (NHS), on various cellular functions of human umbilical vein endothelial cells (HUVECs) in vitro. Compared to non-crosslinked type I collagen, proliferation of seeded endothelial cells was significantly increased on EDC/NHS-crosslinked collagen. Furthermore, higher cell numbers were found with increasing crosslink densities. Neither the morphology of the cells nor the secretion of prostacyclin (PGI2), von Willebrand factor (vWF), tissue plasminogen activator (t-PA) and plasminogen activator inhibitor (PAI-1) was affected by the crosslink density of the collagen substrate. Therefore, EDC/NHScrosslinked collagen is candidate substrate for in vivo application such as endothelial cell seeding of collagen-coated vascular grafts.

Endothelial cell seeding of polytetrafluoroethylene vascular grafts in humans: A preliminary report

1987 ◽  
Vol 6 (6) ◽  
pp. 535-541 ◽  
Author(s):  
Peter Zilla ◽  
Roland Fasol ◽  
Manfred Deutsch ◽  
Teddy Fischlein ◽  
Erich Minar ◽  
...  
Keyword(s):  
Endothelial Cell ◽  
Preliminary Report ◽  
Vascular Grafts ◽  
Cell Seeding ◽  
Endothelial Cell Seeding

An improved method for endothelial cell seeding on polytetrafluoroethylene small caliber vascular grafts

1992 ◽  
Vol 15 (4) ◽  
pp. 649-656 ◽  
Author(s):  
Aviva Schneider ◽  
Raphael N. Melmed ◽  
Herzl Schwalb ◽  
Matthias Karck ◽  
Israel Vlodavsky ◽  
...  
Keyword(s):  
Endothelial Cell ◽  
Vascular Grafts ◽  
Cell Seeding ◽  
Improved Method ◽  
Endothelial Cell Seeding

Fibrinolysis Profiles and Platelet Activation After Endothelial Cell Seeding of Prosthetic Vascular Grafts

1995 ◽  
Vol 9 (6) ◽  
pp. 542-546 ◽  
Author(s):  
J.V. Smyth ◽  
M. Welch ◽  
H.M.H. Carr ◽  
P.D.F. Dodd ◽  
P.R. Eisenberg ◽  
...  
Keyword(s):  
Endothelial Cell ◽  
Platelet Activation ◽  
Vascular Grafts ◽  
Cell Seeding ◽  
Endothelial Cell Seeding

scholarly journals Endothelial Cell Seeding of Small Diameter Vascular Grafts

1990 ◽  
Vol 14 (5) ◽  
pp. 355-360 ◽  
Author(s):  
N. L. James ◽  
K. Schindhelm ◽  
P. Slowiaczek ◽  
B. K. Milthorpe ◽  
N. P. B. Dudman ◽  
...  
Keyword(s):  
Endothelial Cell ◽  
Vascular Grafts ◽  
Small Diameter ◽  
Cell Seeding ◽  
Endothelial Cell Seeding ◽  
Small Diameter Vascular Grafts
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