Endothelial cell Seeding: Coating Dacron and Expanded Polytetrafluoroethylene Vascular Grafts with a Biological Glue Allows Adhesion and Growth of Human Saphenous Vein Endothelial cells

SummaryEndothelial cell seeding, a promising method to improve the performance of small-diameter vascular grafts, requires a suitable substrate, such as crosslinked collagen. Commonly used crosslinking agents such as glutaraldehyde and formaldehyde cause, however, cytotoxic reactions and thereby hamper endothelialization of currently available collagen-coated vascular graft materials.The aim of this study was to investigate the effects of an alternative method for crosslinking of collagen, using N-(3-dimethylaminopropyl)-N’-ethylcarbodiimide (EDC) in combination with N-hydroxysuccinimide (NHS), on various cellular functions of human umbilical vein endothelial cells (HUVECs) in vitro. Compared to non-crosslinked type I collagen, proliferation of seeded endothelial cells was significantly increased on EDC/NHS-crosslinked collagen. Furthermore, higher cell numbers were found with increasing crosslink densities. Neither the morphology of the cells nor the secretion of prostacyclin (PGI2), von Willebrand factor (vWF), tissue plasminogen activator (t-PA) and plasminogen activator inhibitor (PAI-1) was affected by the crosslink density of the collagen substrate. Therefore, EDC/NHScrosslinked collagen is candidate substrate for in vivo application such as endothelial cell seeding of collagen-coated vascular grafts.

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High stretch induces endothelial dysfunction accompanied by oxidative stress and actin remodeling in human saphenous vein endothelial cells

Scientific Reports ◽

10.1038/s41598-021-93081-3 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

T. Girão-Silva ◽

M. H. Fonseca-Alaniz ◽

J. C. Ribeiro-Silva ◽

J. Lee ◽

N. P. Patil ◽

...

Keyword(s):

Oxidative Stress ◽

Endothelial Cells ◽

Endothelial Cell ◽

Endothelial Dysfunction ◽

Saphenous Vein ◽

Saphenous Vein Graft ◽

Artery Bypass ◽

Bypass Graft ◽

Human Saphenous Vein ◽

Actin Remodeling

AbstractThe rate of the remodeling of the arterialized saphenous vein conduit limits the outcomes of coronary artery bypass graft surgery (CABG), which may be influenced by endothelial dysfunction. We tested the hypothesis that high stretch (HS) induces human saphenous vein endothelial cell (hSVEC) dysfunction and examined candidate underlying mechanisms. Our results showed that in vitro HS reduces NO bioavailability, increases inflammatory adhesion molecule expression (E-selectin and VCAM1) and THP-1 cell adhesion. HS decreases F-actin in hSVECs, but not in human arterial endothelial cells, and is accompanied by G-actin and cofilin’s nuclear shuttling and increased reactive oxidative species (ROS). Pre-treatment with the broad-acting antioxidant N-acetylcysteine (NAC) supported this observation and diminished stretch-induced actin remodeling and inflammatory adhesive molecule expression. Altogether, we provide evidence that increased oxidative stress and actin cytoskeleton remodeling play a role in HS-induced saphenous vein endothelial cell dysfunction, which may contribute to predisposing saphenous vein graft to failure.

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Endothelial cell seeding of vascular grafts: Experimental investigations and clinical update

Cardiovascular Pathology ◽

10.1016/s1054-8807(96)90000-9 ◽

1996 ◽

Vol 5 (5) ◽

pp. 296 ◽

Cited By ~ 1

Author(s):

Manfred Deutsch ◽

Johann Meinhart ◽

Teddy Fischlein ◽

Peter Zilla

Keyword(s):

Endothelial Cell ◽

Vascular Grafts ◽

Experimental Investigations ◽

Cell Seeding ◽

Endothelial Cell Seeding

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Endothelial Cell Seeding on PTFE Vascular Prostheses Using a Standardized Seeding Technique

The International Journal of Artificial Organs ◽

10.1177/039139888901200410 ◽

1989 ◽

Vol 12 (4) ◽

pp. 270-275 ◽

Cited By ~ 3

Author(s):

J. Gerlach ◽

K.-M. Kreusel ◽

H.H. Schauwecker ◽

E.S. Bücherl

Keyword(s):

Endothelial Cells ◽

Rotation Speed ◽

Vascular Grafts ◽

Cell Number ◽

Cell Seeding ◽

Vascular Prostheses ◽

Adhesion Rate ◽

Endothelial Cell Seeding ◽

Kinetics Of ◽

Graft Diameter

A standardized method was developed for seeding endothelial cells (EC) in tubular vascular grafts. A rotational cell seeding device for tubular prostheses is presented and parameters influencing the kinetics of cell adhesion (rotation speed, graft diameter, cell suspension level, inoculated cell number) are reported. Seeding EC in 14 mm ID PTFE vascular grafts with rotation rate of 10 rph gave an adhesion rate of 80% in a homogeneous monolayer.

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