Structure of the human amnion: A new perspective on mesenchymal cell/epithelial cell interactions

1998 ◽  
Vol 5 (1) ◽  
pp. 72A-72A ◽  
Author(s):  
J HEAD ◽  
M CASEY ◽  
P MACDONALD
Keyword(s):  
Epithelial Cell ◽  
Mesenchymal Cell ◽  
Cell Interactions ◽  
Human Amnion ◽  
New Perspective

Mesenchyme-mediated effects of retinoic acid during rat intestinal development

1997 ◽  
Vol 110 (10) ◽  
pp. 1227-1238 ◽  
Author(s):  
M. Plateroti ◽  
J.N. Freund ◽  
C. Leberquier ◽  
M. Kedinger
Keyword(s):  
Retinoic Acid ◽  
Epithelial Cell ◽  
Polyclonal Antibodies ◽  
Mesenchymal Cell ◽  
Mesenchymal Cells ◽  
Cell Polarization ◽  
Cell Interactions ◽  
Intestinal Cell ◽  
Cell Phenotype ◽  
Intestinal Development

In previous experiments we showed that intestinal development was dependent upon epithelial-mesenchymal cell interactions. The aim of this study was to investigate the possible role of retinoic acid (RA), a morphogenetic and differentiating agent, on the gut epithelial-mesenchymal unit. For this purpose we first analyzed the effects of a physiological dose of RA on 14-day fetal rat intestine using short-term organ culture experiments, or long-term grafts under the skin of nude mice. In these conditions, RA accelerated villus outgrowth and epithelial cell differentiation as assessed by the onset of lactase expression, and it also stimulated muscle and crypt formation. In order to analyze potential effects of RA mediated by mesenchymal cells, we isolated and characterized gut mucosa mesenchyme-derived cell cultures (mesenchyme-derived intestinal cell lines, MIC). These cells were shown to express mRNAs for retinoid binding proteins similar to those expressed in situ in the intestinal mesenchyme. MIC cells co-cultured with 14-day intestinal endoderms promoted endodermal cell adhesion and growth, and the addition of exogeneous RA enhanced epithelial cell polarization and differentiation assessed by cytokeratin and lactase immunostaining. Such a differentiating effect of RA was not observed on endodermal cells when cultured without a mesenchymal feeder layer or maintained in conditioned medium from RA-treated MIC cells. In the co-cultures, immunostaining of laminin and collagen IV with polyclonal antibodies, as well as alpha1 and beta1 laminin chains mRNAs (analyzed by RT-PCR) increased concurrently with the RA-enhanced differentiation of epithelial cells. It is worth noting that this stimulation by RA was also obvious on the mesenchymal cells cultured alone. These results show that RA plays a role in intestinal morphogenesis and differentiation. In addition, they indicate that RA acts on the mesenchymal cell phenotype and suggest that RA may modify the mesenchymal-epithelial cell interactions during intestinal development.

Large Vulvar Lipoma in an Adolescent: A Case Report

JMS SKIMS ◽  
2011 ◽  
Vol 14 (1) ◽  
pp. 28-29
Author(s):  
R K Maurya ◽  
Pawan Kumar Singh ◽  
Sandeep Singh
Keyword(s):  
Case Report ◽  
Epithelial Cell ◽  
Adolescent Girl ◽  
Rare Case ◽  
Mesenchymal Cell ◽  
Benign Tumors ◽  
Melanocytic Tumors

Lipomas of vulva have been reported only rarely. Benign tumors of the vulva are normally classified according to their origin as epithelial cell tumors (e.g., keratinocytic, adnexal and ectopic tumors), or mesenchymal cell tumors (e.g., vascular, fibrous, muscular, neural, adipose and melanocytic tumors). Vulvar lipomas need to be differentiated from liposarcomas, which are rare but are very similar to lipomas clinically. Here we present a rare case of large vulvar lipoma in an adolescent girl. JMS 2011;14(1):28-29

Increased epithelial-free areas in thymuses with altered EphB-mediated thymocyte–thymic epithelial cell interactions

2017 ◽  
Vol 148 (4) ◽  
pp. 381-394 ◽  
Author(s):  
Javier García-Ceca ◽  
Sara Montero-Herradón ◽  
David Alfaro ◽  
Agustín G. Zapata
Keyword(s):  
Epithelial Cell ◽  
Cell Interactions ◽  
Thymic Epithelial Cell

scholarly journals Transgenic Expression of VEGF in Intestinal Epithelium Drives Mesenchymal Cell Interactions and Epithelial Neoplasia

2009 ◽  
Vol 136 (2) ◽  
pp. 596-606.e4 ◽  
Author(s):  
Amelie Boquoi ◽  
Rodrigo Jover ◽  
Tina Chen ◽  
Marieke Pennings ◽  
Greg H. Enders
Keyword(s):  
Intestinal Epithelium ◽  
Mesenchymal Cell ◽  
Cell Interactions ◽  
Transgenic Expression

scholarly journals Inverse-power-law behavior of cellular motility reveals stromal–epithelial cell interactions in 3D co-culture by OCT fluctuation spectroscopy

Optica ◽  
2015 ◽  
Vol 2 (10) ◽  
pp. 877 ◽  
Author(s):  
Amy L. Oldenburg ◽  
Xiao Yu ◽  
Thomas Gilliss ◽  
Oluwafemi Alabi ◽  
Russell M. Taylor ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Power Law ◽  
Cell Interactions ◽  
Inverse Power ◽  
Cellular Motility ◽  
Inverse Power Law

scholarly journals UDCA modulates monocyte/epithelial cell interactions

2015 ◽  
Vol 29 (S1) ◽  
Author(s):  
Aoife O Dwyer ◽  
Joseph Ward ◽  
Catherine Greene ◽  
Stephen Keely
Keyword(s):  
Epithelial Cell ◽  
Cell Interactions

scholarly journals Ultrastructural observations on cellular and subcellular aspects of experimental Mycoplasma pneumoniae disease

1980 ◽  
Vol 29 (3) ◽  
pp. 1117-1124
Author(s):  
J L Carson ◽  
A M Collier ◽  
S C Hu
Keyword(s):  
Epithelial Cell ◽  
Mycoplasma Pneumoniae ◽  
Freeze Fracture ◽  
Ultrastructural Organization ◽  
Organ Cultures ◽  
Distinct Cell ◽  
Membrane Blebs ◽  
Cell Cytosol ◽  
Subsequent Loss ◽  
New Perspective

The ultrastructural organization of Mycoplasma pneumoniae membranes and spatial relationships of this pathogen to epithelial cells in tracheal organ cultures were examined ultrastructurally by freeze-fracture techniques. Areas of morphologically distinct cell membrane variability characterized by membrane blebs and altered distributions of membrane associated particles were observed in replicas of M. pneumoniae cells. Inspection of the host tracheal epithelium demonstrated the alignment of M. pneumoniae to the epithelium with an accompanying deterioration in the integrity of the lumenal surface membranes and subsequent loss of the epithelial cell cytosol. Ciliary dysfunction was suggested by the observation of ciliary lesions and of disorganized epithelial cell cilia. The methodology used in these studies has permitted a new perspective of host-pathogen interactions at both the cellular and subcellular levels in tracheal organ cultures. These studies may also illustrate ultrastructural correlates of the alteration of host macromolecular synthesis in experimental M. pneumoniae infection.

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