Detection of HIV-1 and HCV Infections Among Antibody-Negative Blood Donors by Nucleic Acid–Amplification Testing

2006 ◽  
Vol 2006 ◽  
pp. 308-309
Author(s):  
M.G. Bissell
Keyword(s):  
Nucleic Acid ◽  
Blood Donors ◽  
Nucleic Acid Amplification ◽  
Nucleic Acid Amplification Testing ◽  
Hiv 1

Detection of HIV-1 and HCV Infections among Antibody-Negative Blood Donors by Nucleic Acid–Amplification Testing

2004 ◽  
Vol 351 (8) ◽  
pp. 760-768 ◽  
Author(s):  
Susan L. Stramer ◽  
Simone A. Glynn ◽  
Steven H. Kleinman ◽  
D. Michael Strong ◽  
Sally Caglioti ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Blood Donors ◽  
Nucleic Acid Amplification ◽  
Nucleic Acid Amplification Testing ◽  
Hiv 1

Detection of HIV-1 infection in blood donors during the immunological window period using the nucleic acid-amplification technology

2007 ◽  
Vol 17 (3) ◽  
pp. 200-204 ◽  
Author(s):  
P. S. P. Scuracchio ◽  
M. C. C. Poli ◽  
M. M. M. Lemos ◽  
A. G. Oliveira Filho ◽  
N. A. Salles ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Blood Donors ◽  
Nucleic Acid Amplification ◽  
Window Period ◽  
Nucleic Acid Amplification Technology ◽  
Hiv 1

scholarly journals Evaluation of Pooling Strategies for Acute HIV-1 Infection Screening Using Nucleic Acid Amplification Testing

2011 ◽  
Vol 49 (10) ◽  
pp. 3667-3668 ◽  
Author(s):  
T. J. Sullivan ◽  
P. Patel ◽  
A. Hutchinson ◽  
S. F. Ethridge ◽  
M. M. Parker
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acid Amplification ◽  
Nucleic Acid Amplification Testing ◽  
Pooling Strategies ◽  
Acute Hiv ◽  
Hiv 1

Nucleic acid amplification testing detection of an HIV-1 infection in a blood donor during the preseroconversion window period

2007 ◽  
Vol 17 (2) ◽  
pp. 147-148 ◽  
Author(s):  
Y. Piquet ◽  
Z. Ivanovic ◽  
S. Laperche ◽  
J. Pillonel ◽  
G. Cristol ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Blood Donor ◽  
Nucleic Acid Amplification ◽  
Window Period ◽  
Nucleic Acid Amplification Testing ◽  
Hiv 1

scholarly journals Fourth-Generation ELISA Screening of Blood Donors: A Reliable Alternative to Nucleic Acid Testing

2021 ◽  
Vol 4 (1) ◽  
pp. 97-104
Author(s):  
Z Ayuba ◽  
OD Damulak ◽  
L Salawu ◽  
ED Jatau ◽  
J Jasini ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Blood Transfusion ◽  
Blood Donors ◽  
Nucleic Acid Amplification ◽  
Fourth Generation ◽  
Research Centre ◽  
Nucleic Acid Amplification Testing ◽  
Resource Limited ◽  
Blood Transfusion Service ◽  
P24 Antigen

The gold standard for HIV screening of blood donors is individual nucleic acid amplification testing (NAT). However, individual NAT testing is cost-prohibitive, especially in a resource-limited setting. The fourth-generation ELISA that detects both p24 antigen and antibody to HIV-1 and 2 has been recommended as the minimum test for HIV to enhance blood transfusion safety and can be an alternative to NAT testing in resource-limited settings. The aim was to assess the performance of a fourth-generation ELISA in use at a regional blood transfusion service using nucleic acid amplification testing on units of screened blood negative to HIV. The study was a cross-sectional study conducted at the National Blood Transfusion Service center and the Plateau State Virology Research Centre, both in Jos, Nigeria. Between August and October 2016, one thousand and eighteen voluntary blood donors were recruited consecutively and had their samples tested using fourth-generation ELISA. One thousand p24 antigen-negative samples were pooled for NAT in an aliquot of 50 samples. All the pools of fifty samples of 1,000 HIV p24 antigen-antibody negative donor blood screened by the fourth-generation ELISA tested negative for HIV RNA on nucleic acid amplification. The yield of pooled NAT for HIV after a fourth-generation ELISA screening of blood donors was found to be zero in this study, thus establishing the fourth-generation ELISA's reliability. Therefore, we recommend adopting the fourth-generation ELISA test as a minimum requirement for blood donor screening.

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