Seeing the light: calcium imaging in cells for drug discovery

2000 ◽  
Vol 5 ◽  
pp. 15-19 ◽  
Author(s):  
Larry C Mattheakis ◽  
Lynne D Ohler
Keyword(s):  
Drug Discovery ◽  
Calcium Imaging ◽  
In Cells

scholarly journals An expanding role for cell biologists in drug discovery and pharmacology

2012 ◽  
Vol 23 (21) ◽  
pp. 4162-4164 ◽  
Author(s):  
Peter K. Sorger ◽  
Birgit Schoeberl
Keyword(s):  
Drug Discovery ◽  
Pharmaceutical Industry ◽  
Drug Development ◽  
Basic Structure ◽  
Healthcare Systems ◽  
New Drugs ◽  
Conceptual Basis ◽  
The Face ◽  
Programming Skills ◽  
In Cells

The profound challenges facing clinicians, who must prescribe drugs in the face of dramatic variability in response, and the pharmaceutical industry, which must develop new drugs despite ever-rising costs, represent opportunities for cell biologists interested in rethinking the conceptual basis of pharmacology and drug discovery. Much better understanding is required of the quantitative behaviors of networks targeted by drugs in cells, tissues, and organisms. Cell biologists interested in these topics should learn more about the basic structure of drug development campaigns and hone their quantitative and programming skills. A world of conceptual challenges and engaging industry–academic collaborations awaits, all with the promise of delivering real benefit to patients and strained healthcare systems.

scholarly journals Calcium imaging for analgesic drug discovery

2022 ◽  
pp. 100083
Author(s):  
Federico Iseppon ◽  
John E. Linley ◽  
John N. Wood
Keyword(s):  
Drug Discovery ◽  
Calcium Imaging ◽  
Analgesic Drug

scholarly journals Golgi Recruitment Assay for Visualizing Small-Molecule Ligand–target Engagement in Cells

2020 ◽  
Author(s):  
Sachio Suzuki ◽  
Masahiro Ikuta ◽  
Tatsuyuki Yoshii ◽  
Akinobu Nakamura ◽  
Keiko Kuwata ◽  
...  
Keyword(s):  
Drug Discovery ◽  
Ligand Binding ◽  
Drug Target ◽  
Chemical Biology ◽  
Protein Isoforms ◽  
Target Engagement ◽  
Ligand Affinity ◽  
Important Challenge ◽  
Small Molecule Ligand ◽  
In Cells

<b>The development of methods that allow detection of ligand–target engagement in cells is an important challenge in chemical biology and drug discovery. Here, we present a Golgi recruitment (G-REC) assay in which the ligand binding to the target protein can be visualized as Golgi-localized fluorescence signals. We show that the G-REC assay is applicable to the detection of various ligand–target interactions, ligand affinity comparison among distinct protein isoforms, and the monitoring of unmodified drug–target engagement in cells.</b>

Bioorthogonal Probes for the Study of MDM2-p53 Inhibitors in Cells and Development of High-Content Screening Assays for Drug Discovery

2016 ◽  
Vol 55 (52) ◽  
pp. 16026-16030 ◽  
Author(s):  
Pier Luca D'Alessandro ◽  
Nicole Buschmann ◽  
Markus Kaufmann ◽  
Pascal Furet ◽  
Frederic Baysang ◽  
...  
Keyword(s):  
Drug Discovery ◽  
High Content Screening ◽  
Screening Assays ◽  
In Cells

scholarly journals Golgi Recruitment Assay for Visualizing Small-Molecule Ligand–target Engagement in Cells

2020 ◽  
Author(s):  
Sachio Suzuki ◽  
Masahiro Ikuta ◽  
Tatsuyuki Yoshii ◽  
Akinobu Nakamura ◽  
Keiko Kuwata ◽  
...  
Keyword(s):  
Drug Discovery ◽  
Ligand Binding ◽  
Drug Target ◽  
Chemical Biology ◽  
Protein Isoforms ◽  
Target Engagement ◽  
Ligand Affinity ◽  
Important Challenge ◽  
Small Molecule Ligand ◽  
In Cells

<b>The development of methods that allow detection of ligand–target engagement in cells is an important challenge in chemical biology and drug discovery. Here, we present a Golgi recruitment (G-REC) assay in which the ligand binding to the target protein can be visualized as Golgi-localized fluorescence signals. We show that the G-REC assay is applicable to the detection of various ligand–target interactions, ligand affinity comparison among distinct protein isoforms, and the monitoring of unmodified drug–target engagement in cells.</b>

Towards a high-throughput drug discovery platform for the screening of GPCR targets in cells

2011 ◽  
Author(s):  
D M F Prazeres ◽  
S A M Martins ◽  
J R C Trabuco ◽  
G A Monteiro ◽  
A Juskowiak ◽  
...  
Keyword(s):  
Drug Discovery ◽  
High Throughput ◽  
In Cells

scholarly journals Calcium Imaging in Drug Discovery for Psychiatric Disorders

2020 ◽  
Vol 11 ◽  
Author(s):  
Saurav Seshadri ◽  
Daniel J. Hoeppner ◽  
Katsunori Tajinda
Keyword(s):  
Drug Discovery ◽  
Psychiatric Disorders ◽  
Calcium Imaging

Bioorthogonal Probes for the Study of MDM2-p53 Inhibitors in Cells and Development of High-Content Screening Assays for Drug Discovery

2016 ◽  
Vol 128 (52) ◽  
pp. 16260-16264 ◽  
Author(s):  
Pier Luca D'Alessandro ◽  
Nicole Buschmann ◽  
Markus Kaufmann ◽  
Pascal Furet ◽  
Frederic Baysang ◽  
...  
Keyword(s):  
Drug Discovery ◽  
High Content Screening ◽  
Screening Assays ◽  
In Cells

Cytoplasmic Tubules in Cells Infected with Laryngotracheitis Virus

1969 ◽  
Vol 27 ◽  
pp. 376-377
Author(s):  
A. M. Watrach
Keyword(s):  
Tissue Culture ◽  
Small Proportion ◽  
Chicken Embryo ◽  
Culture Cells ◽  
Tissue Culture Cells ◽  
Morphologic Characteristics ◽  
Infectious Laryngotracheitis ◽  
Laryngotracheitis Virus ◽  
In Cells

During a study of the development of infectious laryngotracheitis (LT) virus in tissue culture cells, unusual tubular formations were found in the cytoplasm of a small proportion of the affected cells. It is the purpose of this report to describe the morphologic characteristics of the tubules and to discuss their possible association with the development of virus.The source and maintenance of the strain of LT virus have been described. Prior to this study, the virus was passed several times in chicken embryo kidney (CEK) tissue culture cells.

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