Metabolic activation of heterocyclic amines and other procarcinogens in Salmonella typhimurium umu tester strains expressing human cytochrome P4501A1, 1A2, 1B1, 2C9, 2D6, 2E1, and 3A4 and human NADPH-P450 reductase and bacterial O-acetyltransferase

2001 ◽  
Vol 492 (1-2) ◽  
pp. 81-90 ◽  
Author(s):  
Yoshimitsu Oda ◽  
Pramod Aryal ◽  
Takao Terashita ◽  
Elizabeth M.J Gillam ◽  
F.Peter Guengerich ◽  
...  
Keyword(s):  
Salmonella Typhimurium ◽  
Metabolic Activation ◽  
Heterocyclic Amines ◽  
Cytochrome P4501a1 ◽  
P450 Reductase ◽  
Human Cytochrome

scholarly journals Metabolic activation of N -alkylnitrosamines in genetically engineered Salmonella typhimurium expressing CYP2E1 or CYP2A6 together with human NADPH-cytochrome P450 reductase

2000 ◽  
Vol 21 (6) ◽  
pp. 1227-1232 ◽  
Author(s):  
Hirotaka Kushida ◽  
Ken-ichi Fujita ◽  
Akihiro Suzuki ◽  
Masami Yamada ◽  
Toru Endo ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Salmonella Typhimurium ◽  
Metabolic Activation ◽  
Genetically Engineered ◽  
Cytochrome P450 Reductase ◽  
Nadph Cytochrome ◽  
P450 Reductase ◽  
Nadph Cytochrome P450 Reductase

Metabolic activation of carcinogenic 1-nitropyrene by human cytochrome P450 1B1 in Salmonella typhimurium strain expressing an O-acetyltransferase in SOS/umu assay

2001 ◽  
Vol 497 (1-2) ◽  
pp. 223-233 ◽  
Author(s):  
Naoya Hatanaka ◽  
Hiroshi Yamazaki ◽  
Yoshimitsu Oda ◽  
F.Peter Guengerich ◽  
Miki Nakajima ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Salmonella Typhimurium ◽  
Metabolic Activation ◽  
Cytochrome P450 1B1 ◽  
Typhimurium Strain ◽  
Human Cytochrome

scholarly journals Metabolic activation of N-alkylnitrosamines in genetically engineered Salmonella typhimurium expressing CYP2E1 or CYP2A6 together with human NADPH-cytochrome P450 reductase

2000 ◽  
Vol 21 (6) ◽  
pp. 1227-1232 ◽  
Author(s):  
Hirotaka Kushida ◽  
Ken-ichi Fujita ◽  
Akihiro Suzuki ◽  
Masami Yamada ◽  
Toru Endo ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Salmonella Typhimurium ◽  
Metabolic Activation ◽  
Genetically Engineered ◽  
Cytochrome P450 Reductase ◽  
Nadph Cytochrome ◽  
P450 Reductase ◽  
Nadph Cytochrome P450 Reductase

Mutagenicity of some heterocyclic amines in Salmonella typhimurium with metabolic activation by human red blood cell cytosol

1991 ◽  
Vol 261 (4) ◽  
pp. 261-265 ◽  
Author(s):  
M. Duverger-van Bogaert ◽  
M.-C. Crutzen-Fayt ◽  
C. Stecca
Keyword(s):  
Blood Cell ◽  
Salmonella Typhimurium ◽  
Red Blood Cell ◽  
Metabolic Activation ◽  
Heterocyclic Amines ◽  
Cell Cytosol

Genotoxic activation of 2-phenylbenzotriazole-type compounds by human cytochrome P4501A1 and N-acetyltransferase expressed in Salmonella typhimurium umu strains

2008 ◽  
Vol 654 (1) ◽  
pp. 52-57 ◽  
Author(s):  
Yoshimitsu Oda ◽  
Tetsushi Watanabe ◽  
Yoshiyasu Terao ◽  
Haruo Nukaya ◽  
Keiji Wakabayashi
Keyword(s):  
Salmonella Typhimurium ◽  
Cytochrome P4501a1 ◽  
Human Cytochrome

scholarly journals Mutagenicity of carcinogenic heterocyclic amines in Salmonella typhimurium YG strains and transgenic rodents including gpt delta

2021 ◽  
Vol 43 (1) ◽  
Author(s):  
Takehiko Nohmi ◽  
Masahiko Watanabe
Keyword(s):  
Salmonella Typhimurium ◽  
Ames Test ◽  
Point Mutations ◽  
High Sensitivity ◽  
Metabolic Activation ◽  
Reporter Genes ◽  
Heterocyclic Amines ◽  
In Vivo Mutagenicity

AbstractChemical carcinogens to humans have been usually identified by epidemiological studies on the relationships between occupational or environmental exposure to the agents and specific cancer induction. In contrast, carcinogenic heterocyclic amines were identified under the principle that mutagens in bacterial in the Ames test are possible human carcinogens. In the 1970s to 1990s, more than 10 heterocyclic amines were isolated from pyrolysates of amino acids, proteins, meat or fish as mutagens in the Ames test, and they were demonstrated as carcinogens in rodents. In the 1980s and 1990s, we have developed derivatives of the Ames tester strains that overexpressed acetyltransferase of Salmonella typhimurium. These strains such as Salmonella typhimurium YG1024 exhibited a high sensitivity to the mutagenicity of the carcinogenic heterocyclic amines. Because of the high sensitivity, YG1024 and other YG strains were used for various purposes, e.g., identification of novel heterocyclic amines, mechanisms of metabolic activation, comparison of mutagenic potencies of various heterocyclic amines, and the co-mutagenic effects. In the 1990s and 2000s, we developed transgenic mice and rats for the detection of mutagenicity of chemicals in vivo. The transgenics were generated by the introduction of reporter genes for mutations into fertilized eggs of mice and rats. We named the transgenics as gpt delta because the gpt gene of Escherichia coli was used for detection of point mutations such as base substitutions and frameshifts and the red/gam genes of λ phage were employed to detect deletion mutations. The transgenic rodents gpt delta and other transgenics with lacI or lacZ as reporter genes have been utilized for characterization of mutagenicity of heterocyclic amines in vivo. In this review, we summarized the in vitro mutagenicity of heterocyclic amines in Salmonella typhimurium YG strains and the in vivo mutagenicity in transgenic rodents. We discussed the relationships between in vitro and in vivo mutagenicity of the heterocyclic amines and their relations to the carcinogenicity.

Sign in / Sign up

Export Citation Format

Share Document