The synthesis and application of 1-(o-nitrophenyl)-3-(2-thiazolyl)triazene for the determination of palladium(II) by the resonance enhanced Rayleigh light-scattering technique

2000 ◽  
Vol 56 (7) ◽  
pp. 1319-1327 ◽  
Author(s):  
Qiu-E. Cao ◽  
Yunkun Zhao ◽  
Xiaojun Yao ◽  
Zhide Hu ◽  
Qiheng Xu
Keyword(s):  
Light Scattering ◽  
Rayleigh Light Scattering ◽  
Scattering Technique ◽  
Light Scattering Technique ◽  
Rayleigh Light

scholarly journals Determination of Protein in Milk Powder Using 2-Sulfophenylazo-Rhodanine as a Probe by the Enhanced Resonance Rayleigh Light-Scattering Technique

2006 ◽  
Vol 89 (5) ◽  
pp. 1353-1359
Author(s):  
Qin Wei ◽  
Yan Li ◽  
Wenying Dong ◽  
Bin Du
Keyword(s):  
Light Scattering ◽  
Serum Albumin ◽  
Milk Powder ◽  
Buffer Solution ◽  
Protein Determination ◽  
Rayleigh Light Scattering ◽  
Scattering Technique ◽  
Light Scattering Technique ◽  
Rayleigh Light

Abstract In this paper, the interaction between 2-sulfophenylazo-rhodanine and protein was investigated by Rayleigh light-scattering technique. Based on this, a novel method for the determination of protein was developed. The effects of different conditions, such as acidity and media, were investigated thoroughly, and the optimum conditions were confirmed. Bis(2-ethylhexyl)sulfosuccinate (AOT) microemulsion, which is introduced into the protein determination, markedly increased the sensitivity of the system by changing the microenvironment. In pH 2.80 Britton-Robinson buffer solution in the presence of AOT microemulsion, the detection limits of bovine serum albumin, human serum albumin, ovalbumin, and -globulin are 5.4, 4.5, 9.8, and 10.1 ng/mL, respectively. The method developed in this paper has been applied to the determination of protein in milk powder with satisfactory results.

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