The role of the oocyte and oocyte kinases in the development of embryos produced by somatic cell nuclear transfer and its relevance to embryonic stem cell isolation

2006 ◽  
Vol 13 ◽  
pp. 15
Author(s):  
KHS Campbell
Keyword(s):  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Somatic Cell ◽  
Somatic Cell Nuclear Transfer ◽  
Nuclear Transfer ◽  
Embryonic Stem ◽  
Cell Isolation ◽  
Stem Cell Isolation

Rabbit embryonic stem cell lines derived from fertilized, parthenogenetic or somatic cell nuclear transfer embryos

2006 ◽  
Vol 312 (18) ◽  
pp. 3669-3682 ◽  
Author(s):  
Zhen F. Fang ◽  
Hui Gai ◽  
You Z. Huang ◽  
Shan G. Li ◽  
Xue J. Chen ◽  
...  
Keyword(s):  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Somatic Cell ◽  
Cell Lines ◽  
Somatic Cell Nuclear Transfer ◽  
Nuclear Transfer ◽  
Embryonic Stem ◽  
Stem Cell Lines

Embryonic Stem Cell Lines from Somatic Cell Nuclei Via Nuclear Transfer

2005 ◽  
Vol 22 (3) ◽  
pp. 152-158
Author(s):  
Sayaka Wakayama ◽  
Masashi Miyake ◽  
Teruhiko Wakayama
Keyword(s):  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Somatic Cell ◽  
Cell Lines ◽  
Nuclear Transfer ◽  
Embryonic Stem ◽  
Cell Nuclei ◽  
Stem Cell Lines

scholarly journals Advance in the Role of Epigenetic Reprogramming in Somatic Cell Nuclear Transfer-Mediated Embryonic Development

2021 ◽  
Vol 2021 ◽  
pp. 1-13
Author(s):  
Xiaolei Zhang ◽  
Shaorong Gao ◽  
Xiaoyu Liu
Keyword(s):  
Somatic Cell ◽  
Somatic Cell Nuclear Transfer ◽  
Nuclear Transfer ◽  
Embryonic Stem Cell Line ◽  
Embryonic Stem ◽  
Blastocyst Development ◽  
Low Efficiency ◽  
Nuclear Transfer Embryo

Somatic cell nuclear transfer (SCNT) enables terminally differentiated somatic cells to gain totipotency. Many species are successfully cloned up to date, including nonhuman primate. With this technology, not only the protection of endangered animals but also human therapeutics is going to be a reality. However, the low efficiency of the SCNT-mediated reprogramming and the defects of extraembryonic tissues as well as abnormalities of cloned individuals limit the application of reproductive cloning on animals. Also, due to the scarcity of human oocytes, low efficiency of blastocyst development and embryonic stem cell line derivation from nuclear transfer embryo (ntESCs), it is far away from the application of this technology on human therapeutics to date. In recent years, multiple epigenetic barriers are reported, which gives us clues to improve reprogramming efficiency. Here, we reviewed the reprogramming process and reprogramming defects of several important epigenetic marks and highlighted epigenetic barriers that may lead to the aberrant reprogramming. Finally, we give our insights into improving the efficiency and quality of SCNT-mediated reprogramming.

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