A Prototype Model for Indole-3-Acetic Acid (IAA) Production by Azospirillum brasilense SP245

2004 ◽  
Vol 37 (9) ◽  
pp. 493-498 ◽  
Author(s):  
Ilse Y. Smets ◽  
Kristel Bernaerts ◽  
Astrid Cappuyns ◽  
Ositadinma Ona ◽  
Jos Vanderleyden ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Azospirillum Brasilense ◽  
Prototype Model ◽  
Iaa Production ◽  
Indole 3 Acetic Acid

scholarly journals Volatile 1-octanol of tea (Camellia sinensis L.) fuels cell division and indole-3-acetic acid production in phylloplane isolate Pseudomonas sp. NEEL19

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Poovarasan Neelakandan ◽  
Chiu-Chung Young ◽  
Asif Hameed ◽  
Yu-Ning Wang ◽  
Kui-Nuo Chen ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Cell Division ◽  
Petri Dish ◽  
Gas Chromatography Mass Spectrometry ◽  
Dependent Manner ◽  
Tea Leaves ◽  
Acetic Acid Production ◽  
Iaa Production ◽  
Solvent Tolerant ◽  
Indole 3 Acetic Acid

AbstractTea leaves possess numerous volatile organic compounds (VOC) that contribute to tea’s characteristic aroma. Some components of tea VOC were known to exhibit antimicrobial activity; however, their impact on bacteria remains elusive. Here, we showed that the VOC of fresh aqueous tea leaf extract, recovered through hydrodistillation, promoted cell division and tryptophan-dependent indole-3-acetic acid (IAA) production in Pseudomonas sp. NEEL19, a solvent-tolerant isolate of the tea phylloplane. 1-octanol was identified as one of the responsible volatiles stimulating cell division, metabolic change, swimming motility, putative pili/nanowire formation and IAA production, through gas chromatography-mass spectrometry, microscopy and partition petri dish culture analyses. The bacterial metabolic responses including IAA production increased under 1-octanol vapor in a dose-dependent manner, whereas direct-contact in liquid culture failed to elicit such response. Thus, volatile 1-octanol emitting from tea leaves is a potential modulator of cell division, colonization and phytohormone production in NEEL19, possibly influencing the tea aroma.

Characterization of the indole-3-acetic acid (IAA) biosynthetic pathway in an epiphytic strain of Erwinia herbicola and IAA production in vitro

1996 ◽  
Vol 42 (6) ◽  
pp. 586-592 ◽  
Author(s):  
M. Brandi ◽  
E. M. Clark ◽  
S. E. Lindow
Keyword(s):  
Acetic Acid ◽  
Pseudomonas Syringae ◽  
Pyruvic Acid ◽  
Enzyme Assays ◽  
Erwinia Herbicola ◽  
Iaa Production ◽  
Acid Pathway ◽  
Culture Supernatants ◽  
Indole 3 Acetic Acid

An epiphytic strain of Erwinia herbicola (strain 299R) synthesized indole-3-acetic acid (IAA) from indole-3-pyruvic acid and indole-3-acetaldehyde, but not from indole-3-acetamide and other intermediates of various IAA biosynthetic pathways in enzyme assays. TLC, HPLC, and GC–MS analyses revealed the presence of indole-3-pyruvic acid, indole-3-ethanol, and IAA in culture supernatants of strain 299R. Indole-3-acetaldehyde was detected in enzyme assays. Furthermore, strain 299R genomic DNA shared no homology with the iaaM and iaaH genes from Pseudomonas syringae pv. savastanoi, even in Southern hybridizations performed under low-stringency conditions. These observations strongly suggest that unlike gall-forming bacteria which can synthesize IAA by indole-3-acetamide, the indole-3-pyruvic acid pathway is the primary route for IAA biosynthesis in this plant-associated strain. IAA synthesis in tryptophan-supplemented cultures of strain 299R was over 10-fold higher under nitrogen-limiting conditions, indicating a possible role for IAA production by bacterial epiphytes in the acquisition of nutrients during growth in their natural habitat.Key words: indole-3-acetic acid, Erwinia, tryptophan, indole-3-pyruvic acid, nitrogen.

Tryptophan conversion to indole-3-acetic acid via indole-3-acetamide in Azospirillum brasilense Sp7

1993 ◽  
Vol 39 (1) ◽  
pp. 81-86 ◽  
Author(s):  
Tami Bar ◽  
Yaacov Okon
Keyword(s):  
Molecular Weight ◽  
Acetic Acid ◽  
Azospirillum Brasilense ◽  
Soil Bacteria ◽  
Plant Interactions ◽  
Monooxygenase Activity ◽  
Azospirillum Brasilense Sp7 ◽  
Indole 3 Acetic Acid ◽  
Induced Proteins

The phytohormone indole-3-acetic acid is involved in several types of microorganism-plant interactions. In the most widely studied pathway, tryptophan-2-monooxygenase converts tryptophan to the intermediate indole-3-acetamide, and indole-3-acetamide hydrolase catalyzes the conversion of indole-3-acetamide to indole-3-acetic acid. The genetic determinants for these enzymatic conversions are iaaM and iaaH, respectively. This pathway has been observed in many pathogenic and symbiotic soil bacteria. The associative soil bacteria of the genus Azospirillum are known to promote plant growth, probably via the secretion of phytohormones, including indole-3-acetic acid. The following evidence is presented for the existence of the above-described indole-3-acetic acid pathway in Azospirillum brasilense Sp7: the high toxicity of α-methyltryptophan as compared with that of 5-methyltryptophan; indole-3-acetic acid formation in vivo from indole-3-acetamide; the existence of two tryptophan-induced proteins, one of which has a molecular weight similar to that of tryptophan-2-monooxygenase; tryptophan-2-monooxygenase activity observed on nondenaturing gel; the existence of a protein with high tryptophan-2-monooxygenase activity with a molecular weight similar to that of one of the tryptophan-induced proteins on a two-dimensional gel; and the partial homology between the iaaM gene, which encodes tryptophan-2-monooxygenase in Pseudomonas savastanoi, and A. brasilense Sp7 total DNA.Key words: Azospirillum brasilense Sp7, indole-3-acetic acid, tryptophan, indole-3-acetamide.

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