scholarly journals 940. Lentika/3™: Creation and Characterization of High Titer Third Generation Lentivirus Producer Clones in a VSV.G Packaging Cell Line

2002 ◽  
Vol 5 (5) ◽  
pp. S307
2006 ◽  
Vol 14 (2) ◽  
pp. 276-284 ◽  
Author(s):  
Adam S. Cockrell ◽  
Hong Ma ◽  
Kailing Fu ◽  
Thomas J. McCown ◽  
Tal Kafri

1999 ◽  
Vol 73 (1) ◽  
pp. 576-584 ◽  
Author(s):  
Tal Kafri ◽  
Henriette van Praag ◽  
Ling Ouyang ◽  
Fred H. Gage ◽  
Inder M. Verma

ABSTRACT Lentivirus vectors can transduce dividing and nondividing cells. Using three-plasmid transient transfections, high-titer (>109 IU/ml) recombinant lentivirus vectors pseudotyped with vesicular stomatitis virus G (VSV-G) protein can be generated (T. Kafri et al., Nat. Genet. 17:314–317, 1997; H. Miyoshi et al., Proc. Natl. Acad. Sci. USA 94:10319–10323, 1997; L. Naldini et al., Science 272:263–267, 1996). The recombinant lentiviruses can efficiently infect brain, liver, muscle, and retinal tissue in vivo. Furthermore, the transduced tissues demonstrated long-term expression of reporter genes in immunocompetent rodents. We now report the generation of a tetracycline-inducible VSV-G pseudotyped lentivirus packaging cell line which can generate virus particles at titers greater than 106 IU/ml for at least 3 to 4 days. The vector produced by the inducible cell line can be concentrated to titers of 109 IU/ml and can efficiently transduce nondividing cells in vitro and in vivo. The availability of a lentivirus packaging cell line will significantly facilitate the production of high-titer lentivirus vectors for gene therapy and study of human immunodeficiency virus biology.


1989 ◽  
Vol 159 (3) ◽  
pp. 1191-1198 ◽  
Author(s):  
Brian Salmons ◽  
Sylviane Moritz-Legrand ◽  
Iqbal Garcha ◽  
Walter H. Günzburg

2018 ◽  
Vol 51 (1) ◽  
pp. 66-70 ◽  
Author(s):  
Sabrina Ribeiro de Almeida Queiroz ◽  
José Valter Joaquim Silva Júnior ◽  
Andréa Nazaré Monteiro Rangel da Silva ◽  
Amanda Gomes de Oliveira Carvalho ◽  
Jefferson José da Silva Santos ◽  
...  

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