A robot-assisted automated facility was established within a biosafety level 3 laboratory located on a university campus. A unique integration of a Genesis (TECAN) liquid handler into the SAGIAN Core System (Beckman-Coulter) enabled tube liquid handling and transfer of reagents from tubes to microplates. An automated enzyme immunoassay (ELISA) for detecting herpes B virus antibodies was developed. Repetition experiments of the automated ELISA and comparison to the manual ELISA demonstrated the efficacy and high reproducibility of the automated method. It is anticipated that research and education activities will benefit from the establishment of the automated diagnostic laboratory in an academic environment.