This paper investigates closure property of the classes of sets accepted by space-bounded two-dimensional alternating Turing machines (2-atm's) and space-bounded two-dimensional alternating pushdown automata (2-apda's), and space-bounded two-dimensional alternating counter automata (2-aca's). Let L(m, n): N2 → N (N denotes the set of all positive integers) be a function with two variables m (= the number of rows of input tapes) and n (= the number of columns of input tapes). We show that (i) for any function f(m) = o( log m) (resp. f(m) = o( log m/ log log m)) and any monotonic nondecreasing function g(n) space-constructible by a two-dimensional Turing machine (2-Tm) (resp. two-dimensional pushdown automaton (2-pda)), the class of sets accepted by L(m,n) space-bounded 2-atm's (2-apda's) is not closed under row catenation, row + or projection, and (ii) for any function f(m) = o(m/ log ) (resp. for any function f(m) such that log f(m) = o( log m)) and any monotonic nondecreasing function g(n) space-constructible by a two-dimensional counter automaton (2-ca), the class of sets accepted by L(m, n) space-bounded 2-aca's is not closed under row catenation, row + or projection, where L(m, n) = f(m) + g(n) (resp. L(m, n) = f(m) × g(n)).