Collagen Extraction and Stable Isotope Analysis of Small Vertebrate Bones: A Comparative Approach

Radiocarbon ◽  
2016 ◽  
Vol 59 (3) ◽  
pp. 679-694 ◽  
Author(s):  
S Cersoy ◽  
A Zazzo ◽  
M Lebon ◽  
J Rofes ◽  
S Zirah

AbstractBone remains of small vertebrate fossils provide valuable information for paleoenvironmental and paleoclimatic reconstructions. However, direct radiocarbon dating of small vertebrates remains challenging as the extraction of sufficient good quality collagen is required. The efficiency of eight collagen extraction protocols was tested on seven samples, representative of different ages and burial environments, including both macro and small vertebrate taxa. First, the samples were prescreened using attenuated total reflectance–Fourier transform infrared spectroscopy (ATR-FTIR) to quantify collagen content in archaeological bones, revealing that one should be discarded for 14C dating. Then, the quantity of protein extracted (yield) and collagen integrity were checked using conventional elemental analysis. The results show that one protocol was not able to accurately extract collagen from the samples. A soft HCl-based protocol seems more appropriate for the pretreatment of archaeological small mammal bones, whereas a harsher protocol might be more efficient to extract a higher amount of collagen from large mammals as well as amphibian bones. The influence of the tested protocols on carbon and nitrogen isotope values was also investigated. The results showed that isotopic variability, when existing, is related to the interindividual differences rather than the different protocols.

2012 ◽  
Vol 405 (9) ◽  
pp. 2857-2867 ◽  
Author(s):  
Kathrin Schreglmann ◽  
Martina Hoeche ◽  
Sibylle Steinbeiss ◽  
Sandra Reinnicke ◽  
Martin Elsner

2009 ◽  
Vol 44 (4) ◽  
pp. 313-322 ◽  
Author(s):  
Monalisa Elshayeb ◽  
Michael D. MacKinnon ◽  
D. George Dixon ◽  
Michael Power

Abstract One strategy for reclamation of oil sands leases in northern Alberta is the construction of lakes and wetlands by capping oil sands process-affected material (OSPM) with water. To assess this approach, experimental sites containing a range of OSPM have been constructed to monitor the evolution of the resulting aquatic habitats. Stable isotopes of carbon and nitrogen were used to assess the effects of OSPM on aquatic food webs. Carbon and nitrogen isotopic signatures of sediment, dissolved inorganic and organic carbon, particulate organic matter, periphyton, plants, plankton, aquatic invertebrates, and fish were used to assess differences related to the naphthenic acid (NA) concentration in OSPM and reference sites. NAs are a principal contaminant of concern in OSPM. Sites were grouped into low (0 to 4 mg/L), medium (4 to 15 mg/L), and high (>15 mg/L) NA concentrations. There were no significant differences in food web area or length among the three NA groupings. In most cases, carbon isotope analyses of samples from low, medium, and high NA concentration sites were not significantly different, suggesting that OSPM is not a significant contributor to food web carbon sources. Significant differences were found in nitrogen isotope signatures between low, medium, and high NA sites. Ammonia from OSPM is suggested as the main contributor to δ15N enrichment.


2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Alexey G. Nikitin ◽  
Peter Stadler ◽  
Nadezhda Kotova ◽  
Maria Teschler-Nicola ◽  
T. Douglas Price ◽  
...  

AbstractArchaeogenetic research over the last decade has demonstrated that European Neolithic farmers (ENFs) were descended primarily from Anatolian Neolithic farmers (ANFs). ENFs, including early Neolithic central European Linearbandkeramik (LBK) farming communities, also harbored ancestry from European Mesolithic hunter gatherers (WHGs) to varying extents, reflecting admixture between ENFs and WHGs. However, the timing and other details of this process are still imperfectly understood. In this report, we provide a bioarchaeological analysis of three individuals interred at the Brunn 2 site of the Brunn am Gebirge-Wolfholz archeological complex, one of the oldest LBK sites in central Europe. Two of the individuals had a mixture of WHG-related and ANF-related ancestry, one of them with approximately 50% of each, while the third individual had approximately all ANF-related ancestry. Stable carbon and nitrogen isotope ratios for all three individuals were within the range of variation reflecting diets of other Neolithic agrarian populations. Strontium isotope analysis revealed that the ~50% WHG-ANF individual was non-local to the Brunn 2 area. Overall, our data indicate interbreeding between incoming farmers, whose ancestors ultimately came from western Anatolia, and local HGs, starting within the first few generations of the arrival of the former in central Europe, as well as highlighting the integrative nature and composition of the early LBK communities.


2020 ◽  
Vol 34 (18) ◽  
Author(s):  
Kerri J. Smith ◽  
Clive N. Trueman ◽  
Christine A.M. France ◽  
Markus J. Peterson

2015 ◽  
Vol 57 ◽  
pp. 69-79 ◽  
Author(s):  
Jessica A. Pearson ◽  
Amy Bogaard ◽  
Mike Charles ◽  
Simon W. Hillson ◽  
Clark Spencer Larsen ◽  
...  

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