INHERITANCE AND LINKAGE STUDIES WITH THE GRANDPA GENE IN BARLEY, HORDEUM VULGARE L.

1971 ◽  
Vol 13 (3) ◽  
pp. 489-498
Author(s):  
R. W. Matchett ◽  
H. G. Nass ◽  
D. W. Robertson
Keyword(s):  
Hordeum Vulgare ◽  
Chromosomal Location ◽  
Gene Interactions ◽  
Chromosome 2 ◽  
Linkage Studies ◽  
Hordeum Vulgare L ◽  
Barley Genome ◽  
Chlorophyll Mutants ◽  
Mutant Genes ◽  
Linkage Association

This study was initiated to determine the chromosomal location of the grandpa (gp) gene within the barley genome. The gp gene was placed on the long arm of chromosome 2 as indicated by linkage association with liguleless (li).Tests of allelism showed the gp gene to the allelic with the gp-2 gene. Seven sources of "yellow" chlorophyll mutants when crossed to grandpa plants gave albino double recessive seedlings. Three other sources of "yellow" chlorophyll mutants in the double recessive combination with grandpa exhibited yellow and white bands on the leaves. Double recessive individuals carrying the mottled (mt2) and grandpa genes were also albino. This is evidence of gene interactions between chlorophyll mutant genes.

FISH landmarks for barley chromosomes (Hordeum vulgare L.)

Genome ◽  
1999 ◽  
Vol 42 (2) ◽  
pp. 274-281 ◽  
Author(s):  
Susan E Brown ◽  
Janice L Stephens ◽  
Nora LV Lapitan ◽  
Dennis L Knudson
Keyword(s):  
Hordeum Vulgare ◽  
Digital Imaging ◽  
18S Rdna ◽  
Chromosomal Location ◽  
5S Rdna ◽  
Hordeum Vulgare L ◽  
Metaphase Chromosomes ◽  
Bac Clone ◽  
Rdna Probe

Barley metaphase chromosomes (2n = 14) can be identified by fluorescence in situ hybridization (FISH) and digital imaging microscopy using heterologous 18S rDNA and 5S rDNA probe sequences. When these sequences are used together, FISH landmark signals were seen so that all 7 chromosomes were uniquely identified and unambiguously oriented. The chromosomal location of the landmark signals was determined by FISH to a barley trisomic series using the 18S and 5S probes labeled with different fluorophores. The utility of these FISH landmarks for barley physical mapping was also demonstrated when an Amy-2 cDNA clone and a BAC clone were hybridized with the FISH landmark probes.Key words: Hordeum vulgare, barley, FISH, 5S, 18S, rDNA, landmarks, chromosome.

Genetic studies on net blotch resistance in a barley cross

1996 ◽  
Vol 76 (4) ◽  
pp. 715-719 ◽  
Author(s):  
K.M. Ho ◽  
T.M. Choo ◽  
A. Tekauz ◽  
R.A. Martin
Keyword(s):  
Hordeum Vulgare ◽  
Dominant Gene ◽  
Recessive Gene ◽  
Pyrenophora Teres ◽  
Chromosome 2 ◽  
Net Blotch ◽  
Hordeum Vulgare L ◽  
Key Words Barley ◽  
Recessive Genes ◽  
Selection For

An investigation was initiated to study the genetics of resistance to three isolates of Pyrenophora teres (WRS102, WRS858, and WRS857), which have been routinely used for screening for net blotch resistance in Canada. The F1, F2, and doubled-haploid lines were derived from a Leger/CI9831 cross of barley (Hordeum vulgare L.). These materials, along with their parents, were inoculated with each of the three isolates at the three-leaf stage in growth chambers. Results showed that resistance to WRS102 was controlled by three recessive genes, resistance to WRS858 by one recessive gene, and resistance to WRS857 by either one dominant gene or two complementary genes. One of the WRS102-resistance genes appeared to be on chromosome 2 and another linked to the WRS858-resistance gene. Resistance to these three isolates was not associated with awn type, esterase 1, and esterase 5. Selection for resistance to WRS102 and WRS858 would be more effective than selection for resistance to WRS857 in a conventional breeding program. Key words: Barley, Hordeum vulgare, net blotch, Pyrenophora teres, haploids

Physical mapping of four sites of 5S rDNA sequences and one site of the α-amylase-2 gene in barley (Hordeum vulgare)

Genome ◽  
1993 ◽  
Vol 36 (3) ◽  
pp. 517-523 ◽  
Author(s):  
I. J. Leitch ◽  
J. S. Heslop-Harrison
Keyword(s):  
In Situ Hybridization ◽  
Hordeum Vulgare ◽  
Physical Mapping ◽  
5S Rdna ◽  
Chromosome 1 ◽  
Chromosome 2 ◽  
Hordeum Vulgare L ◽  
Rdna Sequences ◽  
5S Rdna Sequence

The 5S rDNA sequences have been mapped on four pairs of barley (Hordeum vulgare L.) chromosomes using in situ hybridization and barley monotelotrisomic lines. The 5S rDNA sequences are located, genetically and physically, on the short arm of chromosome 1 (7I) and the long arms of chromosomes 2 (2I) and 3 (3I). The 5S rDNA sequence is also located on the physically long arm of chromosome 4 (4I). Only one site on chromosome 2(2I) has previously been reported. The characteristic locations of the 5S rDNA sequences make them useful as molecular markers to identify each barley chromosome. The physical position of the low-copy α-amylase-2 gene was determined using in situ hybridization; the location of this gene on the long arm of chromosome 1 (7I) was confirmed by reprobing the same preparation with the 5S rDNA probe. The results show that there is a discrepancy between the physical and genetic position of the α-amylase-2 gene.Key words: genetic mapping, physical mapping, barley, mapping, 5S DNA, α-amylase, in situ hybridization.

Molecular mapping of the shrunken endosperm genes seg8 and sex1 in barley (Hordeum vulgare L.)

Genome ◽  
2006 ◽  
Vol 49 (10) ◽  
pp. 1209-1214 ◽  
Author(s):  
Marion S. Röder ◽  
Christian Kaiser ◽  
Winfriede Weschke
Keyword(s):  
Hordeum Vulgare ◽  
Microsatellite Markers ◽  
Linkage Mapping ◽  
Centromeric Region ◽  
Molecular Mapping ◽  
Recessive Gene ◽  
Rice Chromosome ◽  
Chromosome 2 ◽  
Hordeum Vulgare L ◽  
Causal Genes

A number of mutations affecting seed development in barley (Hordeum vulgare L.) have been known for many years; however, to date, no research has been reported that elucidates the molecular structure of the causal genes. As a first step, we initiated the linkage mapping of the two shrunken endosperm genes seg8 and sex1 using microsatellite markers. The recessive gene seg8 was mapped in the centromeric region of chromosome 7H to a 4.6 cM interval flanked by markers GBM1516 and Bmag341. The recessive sex1 gene showed xenia effects and was located in the centromeric region of barley chromosome 6H, which is in accordance to the previously reported chromosomal location in the classical linkage map. It was flanked by markers GBM5012 and GBM1063 in a 4.2 cM interval. EST-derived microsatellite markers were used to establish the syntenic relationships to the genomic rice sequences. Two orthologous sites on rice chromosome 2 flanking a 4.1 Mb sequence had homology to the respective barley markers in the sex1 region. For the markers in the seg8 region orthologous sites on rice chromosome 6 were detected.

Segregation distortion and linkage studies in microspore-derived double haploid lines of Hordeum vulgare L.

1992 ◽  
Vol 83-83 (6-7) ◽  
pp. 919-924 ◽  
Author(s):  
M. Zivy ◽  
P. Devaux ◽  
J. Blaisonneau ◽  
R. Jean ◽  
H. Thiellement
Keyword(s):  
Hordeum Vulgare ◽  
Segregation Distortion ◽  
Double Haploid ◽  
Linkage Studies ◽  
Hordeum Vulgare L

PRODUCTION, MORPHOLOGY AND MEIOSIS OF RECIPROCAL BARLEY-WHEAT HYBRIDS

1980 ◽  
Vol 22 (1) ◽  
pp. 117-123 ◽  
Author(s):  
George Fedak
Keyword(s):  
Triticum Aestivum ◽  
Hordeum Vulgare ◽  
Chinese Spring ◽  
Reciprocal Cross ◽  
Triticum Aestivum L ◽  
Homoeologous Pairing ◽  
Hordeum Vulgare L ◽  
Barley Genome ◽  
Cytological Abnormalities ◽  
Wheat Hybrids

The intercrossing of wheat (Triticum aestivum L. cv. Chinese Spring) and barley (Hordeum vulgare L. cv. Betzes) yielded hybrids at a frequency of 0.80% of pollinated florets for the barley-wheat combinations and 0.23% for the reciprocal cross. An increase in homoeologous pairing of wheat chromosomes was observed in both hybrids compared with the pairing observed in wheat haploids indicating that the barley genome had pairing promoting properties. Cytological abnormalities such as hyperploid meiotic cells and isochromosomes were attributed to abnormalities at premeiotic mitosis.

Transfer and mapping of the shoot-differentiation locus Shd1 in barley chromosome 2

Genome ◽  
1995 ◽  
Vol 38 (5) ◽  
pp. 1009-1014 ◽  
Author(s):  
T. Komatsuda ◽  
F. Taguchi-Shiobara ◽  
S. Oka ◽  
F. Takaiwa ◽  
T. Annaka ◽  
...  
Keyword(s):  
Hordeum Vulgare ◽  
Immature Embryo ◽  
Gene Products ◽  
Chromosome 2 ◽  
Near Isogenic Lines ◽  
Hordeum Vulgare L ◽  
Isogenic Lines ◽  
Shoot Differentiation ◽  
Backcross Breeding ◽  
Positive Effect

The locus Shd1, which we previously mapped to the long arm of chromosome 2 of Hordeum vulgare L., controls the differentiation of shoots from immature barley embryo callus. The locus has major effects and its action explains more than 65% of the total genetic variance in the shoot-differentiation rate. The allele of cultivar Kanto Nakate Gold designated Shd1K has a significant positive effect on the shoot-differentiation rate, whereas Shd1A of cultivar Azumamugi does not promote shoot differentiation. To identify gene products and characterize the function of Shd1, a set of near-isogenic lines is essential. In this study we produced BC5F1 plants by repeated backcrossing of 'Azumamugi' to F1 plants ('Azumamugi' × 'Kanto Nakate Gold'). The BC5F1 plants were examined for their RFLP genotype and for the shoot-differentiation ability of immature embryo-derived callus. The results indicated that the Shd1 locus was located in a chromosomal region between MWG2081 and MWG503 that flanks the MWG801, cMWG699, v (ear type), and MWG865 loci. Shd1K from 'Kanto Nakate Gold' functions effectively in the genetic background of 'Azumamugi', an indication that backcross breeding is possible for production of near-isogenic lines that would be very suitable for tissue culture.Key words: Hordeum vulgare L., shoot-differentiation ability, immature embryo callus, backcross breeding, RFLPs.

A novel QTL for Septoria speckled leaf blotch resistance in barley (Hordeum vulgare L.) accession PI 643302 by whole-genome QTL mapping

Genome ◽  
2010 ◽  
Vol 53 (8) ◽  
pp. 630-636 ◽  
Author(s):  
G. T. Yu ◽  
J. D. Franckowiak ◽  
S. H. Lee ◽  
R. D. Horsley ◽  
S. M. Neate
Keyword(s):  
Hordeum Vulgare ◽  
Yield Loss ◽  
Wild Barley ◽  
Chromosomal Location ◽  
Interval Mapping ◽  
Distal Region ◽  
Barley Accession ◽  
Hordeum Vulgare L ◽  
Leaf Blotch ◽  
Primary Problem

Septoria speckled leaf blotch (SSLB), caused by Septoria passerinii , is one of the most important foliar diseases of barley ( Hordeum vulgare L.) in North America. The primary problem caused by this disease is substantial yield loss. The objective of this study was to determine the chromosomal location of SSLB resistance genes in the barley accession PI 643302. A recombinant inbred line population was developed from the cross Zhenongda 7/PI 643302. PI 643302 is resistant while Zhenongda 7 is susceptible to SSLB. The population was phenotyped for SSLB resistance in five experiments in the greenhouse. A linkage map comprising 113 molecular markers was constructed and simplified composite interval mapping was performed. Two QTLs, designated QrSp-1H and QrSP-2H, were found. QrSp-1H was found on the short arm of chromosome 1H (1HS) in all five experiments and showed a large effect against SSLB. Based on the location of QrSp-1H, it is likely the SSLB resistance gene Rsp2. The QTL QrSp-2H mapped to the distal region on the long arm of chromosome 2H (2HL), had a smaller effect than QrSp-1H, and was also detected consistently in all five experiments. A QTL for SSLB resistance in the same region on chromosome 2H has not been reported previously in either cultivated or wild barley; thus, QrSp-2H is a new QTL for SSLB resistance in barley.

Comparison and integration of four barley genetic maps

Genome ◽  
1996 ◽  
Vol 39 (2) ◽  
pp. 379-394 ◽  
Author(s):  
Xiaoquan Qi ◽  
Piet Stam ◽  
Pim Lindhout
Keyword(s):  
Hordeum Vulgare ◽  
Region Of Interest ◽  
Genetic Maps ◽  
Data Sets ◽  
Independent Data ◽  
Hordeum Vulgare L ◽  
Integrated Map ◽  
Barley Genome ◽  
The Individual ◽  
Good Agreement

Barley (Hordeum vulgare L.) is one of the most extensively studied food crops in recent molecular research. More than 1000 molecular markers have been located on the barley genome by using five independent populations. For the present study, four segregation data sets, 'Proctor' × 'Nudinka', 'Igri' × 'Franka', 'Steptoe' × 'Morex', and 'Harrington' × TR306, were downloaded from the publicly available GrainGenes databank. Since 22% of the markers are common to at least two of the independent data sets, we were able to establish an integrated map using the computer package JOINMAP v2.0. The integrated map contains 898 markers, covers 1060 cM, and removes many large gaps present in the individual maps. Comparison of the integrated map with the individual maps revealed that the overall linear order of markers is in good agreement and that the integrated map is consistent with the component maps. No significant reordering of markers was found. This conservative property of the barley genome makes the integrated map reliable and successful. Except for chromosome 7 (5H), marker clustering was observed in the centromeric regions, probably owing to the centromeric suppression of recombination. Based on this integrated map, geneticists and breeders can choose their favourite markers in any region of interest of the barley genome. Key words : Hordeum vulgare, RFLP, integrated map.

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