Molecular evidence for the presence of a G7 genotype of Echinococcus granulosus in Slovakia

2000 ◽  
Vol 74 (2) ◽  
pp. 177-181 ◽  
Author(s):  
V. Šnábel ◽  
S. D'Amelio ◽  
K. Mathiopoulos ◽  
L. Turčeková ◽  
P. Dubinský
Keyword(s):  
Echinococcus Granulosus ◽  
Hydatid Disease ◽  
Nadh Dehydrogenase ◽  
Sequence Variation ◽  
Rflp Analysis ◽  
Nucleotide Sequences ◽  
Molecular Evidence ◽  
Its1 Region ◽  
Pcr Rflp ◽  
Local Patterns

AbstractVariability in Echinococcus granulosus is very important epidemiologically since strain characteristics may influence local patterns of transmission of hydatid disease. To classify the genotype presented in pig protoscoleces of the Slovak territory, a DNA-based approach has been used. Nucleotide sequences for a 471 bp region of the mitochondrial NADH dehydrogenase 1 (ND1) gene revealed a substantial affinity of isolates examined to the G7 genotype. Only a 0.9–3.4% sequence variation was recorded for E. granulosus samples compared with the reference G7 variant. To distinguish between G7 and G9 genotypes not differing in ND1 sequences, isolates were additionally examined by PCR-RFLP analysis of the nuclear ITS1 region. The resulting two-banded pattern is characteristic for the G7 strain. The data presented thus provides the first explicit evidence of the G7 genotype in the Slovak region.

scholarly journals Genotyping of Echinococcus granulosus Isolates from Human in Khorasan Province, North-Eastern Iran

2019 ◽  
Author(s):  
Fariba BERENJI ◽  
Seyed Aliakbar SHAMSIAN ◽  
Marziyeh NOURI DALOEE ◽  
Seyed Hossein FATTAHI MASOOM ◽  
Elham MOGHADDAS
Keyword(s):  
Echinococcus Granulosus ◽  
Nadh Dehydrogenase ◽  
Rflp Analysis ◽  
Medical Sciences ◽  
Lung Cysts ◽  
Eastern Iran ◽  
Pcr Rflp ◽  
North Eastern ◽  
Polymerase Chain ◽  
Sheep Strain

Background: Human hydatidosis is endemic in northeastern Iran. The present study aimed to investigate molecular diversity of Echinococcus granulosus isolates collected from human surgically. Methods: Sixty human hydatid cysts (58 lung cysts and 2 liver cysts) were collected through surgery from Ghaem and Emam Reza hospitals in Mashhad University of Medical Sciences during 2015-2016. Cysts were characterized using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis of the internal transcribed spacer 1 (ITS1) gene and sequencing fragments of the genes coding for mitochondrial cytochrome c oxidase subunit 1 (cox1) and NADH dehydrogenase subunit I (nad1). Results: Overall, 55 out of 60 Echinococcus granulosus cysts (91.6%) were determined as the G1 strain, 4 cases (6.6%) were determined as the G6 strain and 1 sample was not identified. Conclusion: Although sheep strain (G1) is dominated in human patients in Great Khorasan, the prevention of camel-dog cycle should pay attention in this region.

Genetic variation and epidemiology of Echinococcus granulosus in Argentina

Parasitology ◽  
1999 ◽  
Vol 118 (5) ◽  
pp. 523-530 ◽  
Author(s):  
M. C. ROSENZVIT ◽  
L.-H. ZHANG ◽  
L. KAMENETZKY ◽  
S. G. CANOVA ◽  
E. A. GUARNERA ◽  
...  
Keyword(s):  
Echinococcus Granulosus ◽  
Rflp Analysis ◽  
Santa Fe ◽  
Cystic Hydatid Disease ◽  
Rio Negro ◽  
Pcr Rflp ◽  
Control Programmes ◽  
The Common ◽  
Cystic Hydatid ◽  
Sheep Strain

Polymerase chain reaction–ribosomal ITS-1 DNA (rDNA) restriction fragment length polymorphism (PCR–RFLP) analysis and sequencing of the mitochondrial cytochrome c oxidase subunit 1 (CO1) and NADH dehydrogenase 1 (ND1) genes were used to characterize 33 Echinococcus granulosus isolates collected from different regions and hosts in Argentina, and to determine which genotypes occurred in humans with cystic hydatid disease. The results of the study demonstrated the presence of at least 4 distinct genotypes; the common sheep strain (G1) in sheep from Chubut Province and in humans from Río Negro Province, the Tasmanian sheep strain (G2) in sheep and 1 human from Tucumán Province, the pig strain (G7) in pigs from Santa Fe Province and the camel strain (G6) in humans from Río Negro and Buenos Aires Provinces. The finding that pigs harboured the pig strain and the occurrence of the Tasmanian sheep strain has considerable implications for the implementation of hydatid control programmes due to the shorter maturation time of both strains in dogs compared with the common sheep strain. Furthermore, this is the first report of the presence of the G2 and G6 genotypes in humans which may also have important consequences for human health.

Presence of Two Plasminogen Alleles in Normal Populations

1998 ◽  
Vol 79 (01) ◽  
pp. 150-154 ◽  
Author(s):  
Masafumi Kida ◽  
Masuyo H.- Kawabata ◽  
Tomio Yamazaki ◽  
Akitada Ichinose
Keyword(s):  
Rflp Analysis ◽  
Normal Subjects ◽  
Nucleotide Sequences ◽  
Normal Populations ◽  
Pcr Rflp ◽  
Expression Activity ◽  
Flanking Regions

SummaryWhen we compared nucleotide sequences of the 5’-flanking regions for plasminogen genes from 11 individuals, six substitutions were identified even among normal subjects. A new haplotype (termed allele II) was screened by PCR-RFLP analysis among 54 Japanese and 58 Caucasian normal subjects. The frequency of allele II was 0.787 in the Japanese and 0.560 in the Caucasians, indicating that the ratio of alleles differs between populations. Examination of 118 cases with dysplasminogenemia revealed that the Ala601-Thr mutation was present on allele I in most cases. This mutation was also associated with allele II in one-fourth of all cases, suggesting possible recombination within the plasminogen gene. Interestingly, we previously demonstrated that the expression activity of allele II was about 1.8 fold that of allele I in vitro.

Identification of razor clams Ensis arcuatus and Ensis siliqua by PCR–RFLP analysis of ITS1 region

2008 ◽  
Vol 74 (3) ◽  
pp. 511-515 ◽  
Author(s):  
Ruth FREIRE ◽  
Juan FERNÁNDEZ-TAJES ◽  
Josefina MÉNDEZ
Keyword(s):  
Rflp Analysis ◽  
Its1 Region ◽  
Ensis Siliqua ◽  
Pcr Rflp ◽  
Razor Clams

Molecular genetic characterization of the cervid strain (‘northern form’) ofEchinococcus granulosus

Parasitology ◽  
1994 ◽  
Vol 109 (2) ◽  
pp. 215-221 ◽  
Author(s):  
J. Bowles ◽  
D. Blair ◽  
D. P. McManus
Keyword(s):  
Genetic Characterization ◽  
Molecular Genetic ◽  
Rflp Analysis ◽  
Domestic Sheep ◽  
Rflp Pattern ◽  
Its1 Region ◽  
Pcr Rflp ◽  
Mitochondrial Nd1 ◽  
Molecular Genetic Characterization

SUMMARYWe have used a number of molecular genetic approaches to characterize the cervid strain (‘northern form’) ofEchinococcus granulosus.PCR–RFLP analysis of the nuclear ITS1 region of the rDNA repeat can readily distinguish the cervid form from other strains ofE. granulosus. The complexity of the RFLP patterns obtained suggests that a number of distinct ITS1 types are present in this strain which may represent an inter-strainE. granulosushybrid. Mitochondrial CO1 sequence of the cervid genotype was ambiguous at 18 positions and closely resembles a cluster of previously characterizedE. granulosusgenotypes, G1 (common, domestic sheep)/G2 (Tasmanian sheep)/G3 (buffalo). In contrast, mitochondrial ND1 sequence, although unique, suggests that the cervid form is most similar to strains represented by the G6 (camel)/G7 (pig) genotypes. We assume that the CO1 and ND1 sequences obtained for the cervid genotype are linked in a single mitochondrial genome although this is difficult to explain if conventional molecular genetics of mitochondrial DNA are assumed. Based on its unique ND1 sequence and ITS1 PCR–RFLP pattern, the cervid strain appears to represent a distinct genotype (designated G8) ofE. granulosus.

Molecular genotyping of Echinococcus granulosus from dromedaries (Camelus dromedarius) in eastern Iran

2013 ◽  
Vol 89 (1) ◽  
pp. 100-104 ◽  
Author(s):  
E. Moghaddas ◽  
H. Borji ◽  
A. Naghibi ◽  
P. Shayan ◽  
G.R. Razmi
Keyword(s):  
Echinococcus Granulosus ◽  
Rflp Analysis ◽  
Camelus Dromedarius ◽  
Public Health Importance ◽  
Morphological Findings ◽  
Eastern Iran ◽  
Morphological Criteria ◽  
Geographical Regions ◽  
Pcr Rflp ◽  
Polymerase Chain

AbstractWith the aim of genotyping Echinococcus granulosus cysts found in Iranian dromedaries (Camelus dromedarius), 50 cysts of E. granulosus were collected from five geographical regions in Iran. Cysts were characterized using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis of the internal transcribed spacer 1 (ITS1) gene and sequencing fragments of the genes coding for mitochondrial cytochrome c oxidase subunit 1 (cox1). Morphological criteria using rostellar hook dimensions were also undertaken. The present results have shown that 27 out of 50 E. granulosus cysts (54%) were determined as the G1 strain, and the other (46%) were determined as the G6 strain. The molecular analysis of the ITS1 region of ribosomal DNA corresponded with the morphological findings. Because of its recognized infectivity in humans, the G1 genotype is a direct threat to human health and its presence in Iranian dromedaries is of urgent public health importance.

PCR–RFLP identification of endemic Chilean species of Rhagoletis (Diptera: Tephritidae) attacking Solanaceae

2002 ◽  
Vol 92 (4) ◽  
pp. 337-341 ◽  
Author(s):  
M. Salazar ◽  
C. Theoduloz ◽  
A. Vega ◽  
F. Poblete ◽  
E. González ◽  
...  
Keyword(s):  
Geographical Distribution ◽  
Endemic Species ◽  
Nadh Dehydrogenase ◽  
Reliable Method ◽  
Nucleotide Sequences ◽  
Target Sequence ◽  
Restriction Maps ◽  
Strong Similarity ◽  
Morphological Criteria ◽  
Pcr Rflp

AbstractFour species of the genus Rhagoletis are native to Chile: R. nova (Schiner), R. conversa, (Brèthes), R. penela Foote and R. tomatis Foote. Currently, identification of these species is based on morphological criteria, but their strong similarity makes precise recognition difficult. To clarify species separation for quarantine purposes, a reliable method based on a PCR–RFLP procedure is reported. A DNA region containing mitochondrial NADH dehydrogenase genes was selected as a target sequence for the analysis. The amplification products (c. 1 kb) were digested with either SspI or DdeI, yielding specific patterns that differentiated each of the endemic species. Complete nucleotide sequences were determined, confirming empirical restriction maps. This report updates information on the geographical distribution of Rhagoletis species in Chile.

scholarly journals Molecular Identification of Agents of Human Cutaneous Leishmaniasis and Canine Visceral Leishmaniasis in Different Areas of Iran Using Internal Transcribed Spacer 1 PCR-RFLP

2018 ◽  
pp. 162-171 ◽  
Author(s):  
Aref Teimouri ◽  
Mehdi Mohebali ◽  
Elham Kazemirad ◽  
Homa Hajjaran
Keyword(s):  
Visceral Leishmaniasis ◽  
Cutaneous Leishmaniasis ◽  
Rflp Analysis ◽  
Canine Visceral Leishmaniasis ◽  
Species Comparison ◽  
Its1 Region ◽  
Pcr Rflp ◽  
Leishmania Spp ◽  
Human Cutaneous Leishmaniasis ◽  
Rflp Assay

Background: Leishmaniasis is a major medical health problem and distributes in nearly half of 31 provinces of Iran. We aimed to identify cutaneous and visceral Leishmania spp. isolated from infected humans and domestic dogs in various regions of Iran, 2010‒2013. Methods: DNA was extracted from 108 lesion exudate samples of suspected patients to cutaneous leishmaniasis and nine liver and spleen aspirates of infected dogs cultured in RPMI-1640 and amplified using partial sequence of ITS1 gene. The PCR amplicons were digested using HaeIII endonuclease enzyme and used in restriction fragment length polymorphism (RFLP) assay. Then, 48 amplicons representing various hosts were sequenced and compared to se­quences from GenBank databases using BLAST. Results: PCR-RFLP analysis showed that 60 and 48 CL patients were infected by Leishmania tropica and L. major, respectively. From nine canine visceral leishmaniasis (CVL) isolates, eight isolates were identified as L. infantum and one as L. tropica. The greatest similarity of 95.7% in ITS1 region was seen between L. infantum and L. major. Furthermore, the lowest similarity with 65.7% was seen between L. tropica and L. major. Intra-species comparison of ITS1 region in L. infantum, L. major and L. tropica isolates were showed 100%, 98.2% and 72.4 % similarities, respectively. Conclusion: PCR-RFLP based on ITS1 region is an appropriate method to distinguish three Leishmania spp. of L. major, L. tropica, and L. infantum. In intra-species comparison of ITS1 region, genotypic variations showed that L. tropica isolates were more heterogeneous than L. major and L. infantum isolates.

scholarly journals Spontaneous Hybridization between Pinus sylvestris L. and P. mugo Turra in Slovakia

2008 ◽  
Vol 57 (1-6) ◽  
pp. 76-82 ◽  
Author(s):  
A. Kormutak ◽  
B. Demankova ◽  
D. Gömöry
Keyword(s):  
Pinus Sylvestris ◽  
Rflp Analysis ◽  
Putative Hybrid ◽  
Molecular Evidence ◽  
Hybrid Swarm ◽  
Cpdna Region ◽  
Pcr Rflp ◽  
Specific Restriction ◽  
Maternal Tree ◽  
Species Specific

AbstractMolecular evidence for spontaneous hybridization between Pinus sylvestris L. and P. mugo Turra in the putative hybrid swarm populations of the species in Slovakia was provided based on PCR-RFLP analysis of the cpDNA trnV-trnH region. Species-specific restriction profiles generated by Hinf I digests of the cpDNA products reliably identified P. sylvestris and P. mugo haplotypes of the embryos from open pollination. Simultaneous analysis of the respective cpDNA region in megagametophytes and embryos of individual seeds along with needles of a given maternal tree has enabled to score either the P. sylvestris or P. mugo haplotypes in the embryos illustrating hybridization patterns between the two species. Data obtained in this way indicate a relatively extensive hybridization which takes place between P. sylvestris and P. mugo. The extent of hybridization varied among populations as evidenced by the 41.1-58.7% proportion of hybrid embryos registered on the locality Habovka, and by the 8.3% and 2.7% proportions of hybrid embryos on the localities Tisovnica and Sucha Hora, respectively. The approach itself is recommended as a convenient method for monitoring the hybridization patterns in sympatric zones of the studied pine species.

Molecular authentication of Thai medicinal plant Khamin khruea by PCR-RFLP analysis

Planta Medica ◽  
2008 ◽  
Vol 74 (09) ◽  
Author(s):  
P Rojsanga ◽  
W Gritsanapan ◽  
W Leelamanit ◽  
S Sukrong
Keyword(s):  
Medicinal Plant ◽  
Rflp Analysis ◽  
Molecular Authentication ◽  
Pcr Rflp
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