scholarly journals The use of cough/nasal swabs in the rapid diagnosis of respiratory syncytial virus infection by the fluorescent antibody technique

1970 ◽  
Vol 68 (2) ◽  
pp. 283-292 ◽  
Author(s):  
Joyce McQuillin ◽  
P. S. Gardner ◽  
Patricia M. sturdy
Keyword(s):  
Respiratory Syncytial Virus ◽  
Virus Infection ◽  
Nasal Swab ◽  
Fluorescent Antibody ◽  
Rapid Diagnosis ◽  
Fluorescent Antibody Technique ◽  
Antibody Technique ◽  
Nasal Swabs ◽  
Respiratory Virus Infection ◽  
Syncytial Virus

SUMMARYThirty-five consecutive infants admitted into hospital in Newcastle upon Tyne with acute respiratory disease had cough/nasal swabs and nasopharyngeal secretions taken. Both types of specimens were examined the fluorescent antibody technique for respiratory syncytial virus; isolation techniques were also used. Twenty-eight specimens of nasopharyngeal secretion were positive, as were 26 of the corresponding cough/nasal swab preparations. Respiratory syncytial virus was isolated from all but one.Sixteen consecutive children who were only suitable for examination cough/nasal swab preparations were also investigated isolation and fluorescent antibody techniques for respiratory syncytial virus. Respiratory syncytial virus was isolated from eight, seven of whom were positive the fluorescent antibody technique. The use of cough/nasal swab preparations stained the fluorescent antibody technique, although not as efficient as nasopharyngeal secretions, may have a place in the rapid diagnosis of respiratory virus infection in older children and children in general practice. The importance of rapid diagnosis for respiratory virus infection in relationship to antiviral therapy was also discussed.

scholarly journals A comparative study of methods for the diagnosis of respiratory virus infections in childhood

1969 ◽  
Vol 67 (4) ◽  
pp. 659-670 ◽  
Author(s):  
Patricia M. Sturdy ◽  
Joyce McQuillin ◽  
P. S. Gardner
Keyword(s):  
Tissue Culture ◽  
Respiratory Syncytial Virus ◽  
Virus Infection ◽  
Respiratory Syncytial Virus Infection ◽  
Fluorescent Antibody ◽  
Health Department ◽  
Fluorescent Antibody Technique ◽  
Antibody Technique ◽  
Nasal Swabs ◽  
Syncytial Virus

SUMMARYNasopharyngeal secretions and cough/nasal swabs were taken from 111 children admitted to hospital in Newcastle upon Tyne with acute lower respiratory disease. A comparison was made between nasopharyngeal secretions and cough/nasal swabs as material for isolation of viruses in tissue culture. These results were, in turn, compared with those obtained by applying a fluorescent antibody technique to the exfoliated cells in the nasopharyngeal secretions for the rapid diagnosis of respiratory syncytial virus infection.More viruses were isolated in tissue culture from nasopharyngeal secretion than from cough/nasal swabs. Further evidence for the superiority of nasopharyngeal secretions was obtained by comparing the virus isolations in the laboratory in 1967 with those in 1968. Respiratory syncytial virus was not only isolated more often but more quickly in tissue culture inoculated with nasopharyngeal secretions.The fluorescent antibody technique not only provided a diagnosis on the patient's day of admission in 95% of those infected with respiratory syncytial virus but also proved to be as sensitive as the culture of nasopharyngeal secretions and considerably more sensitive than the culture of cough/nasal swabs for the diagnosis of respiratory syncytial virus infection.We wish to acknowledge the invaluable help given us by consultants, medical officers, ward sisters and their staff in the paediatric wards of the Child Health Department. We also wish to thank the technical staff in the Department of Virology for their aid.

Value of Rapid Diagnosis of Respiratory Syncytial Virus Infection on Management of Small Infants

1986 ◽  
Vol 25 (8) ◽  
pp. 404-406 ◽  
Author(s):  
Allan D. Friedman ◽  
Shehla H. Naqvi ◽  
Max Q. Arens ◽  
Margaret A. Eyler
Keyword(s):  
Respiratory Syncytial Virus ◽  
Virus Infection ◽  
Respiratory Syncytial Virus Infection ◽  
Rapid Diagnosis ◽  
Syncytial Virus

scholarly journals Respiratory syncytial virus infection enhancesPseudomonas aeruginosabiofilm growth through dysregulation of nutritional immunity

2016 ◽  
Vol 113 (6) ◽  
pp. 1642-1647 ◽  
Author(s):  
Matthew R. Hendricks ◽  
Lauren P. Lashua ◽  
Douglas K. Fischer ◽  
Becca A. Flitter ◽  
Katherine M. Eichinger ◽  
...  
Keyword(s):  
Respiratory Syncytial Virus ◽  
Virus Infection ◽  
Airway Epithelium ◽  
Biofilm Formation ◽  
Respiratory Virus ◽  
Bacterial Biofilm ◽  
Nutritional Immunity ◽  
Rsv Infection ◽  
Respiratory Virus Infection ◽  
Syncytial Virus

Clinical observations link respiratory virus infection andPseudomonas aeruginosacolonization in chronic lung disease, including cystic fibrosis (CF) and chronic obstructive pulmonary disease. The development ofP.aeruginosainto highly antibiotic-resistant biofilm communities promotes airway colonization and accounts for disease progression in patients. Although clinical studies show a strong correlation between CF patients’ acquisition of chronicP.aeruginosainfections and respiratory virus infection, little is known about the mechanism by which chronicP.aeruginosainfections are initiated in the host. Using a coculture model to study the formation of bacterial biofilm formation associated with the airway epithelium, we show that respiratory viral infections and the induction of antiviral interferons promote robust secondaryP.aeruginosabiofilm formation. We report that the induction of antiviral IFN signaling in response to respiratory syncytial virus (RSV) infection induces bacterial biofilm formation through a mechanism of dysregulated iron homeostasis of the airway epithelium. Moreover, increased apical release of the host iron-binding protein transferrin during RSV infection promotesP.aeruginosabiofilm development in vitro and in vivo. Thus, nutritional immunity pathways that are disrupted during respiratory viral infection create an environment that favors secondary bacterial infection and may provide previously unidentified targets to combat bacterial biofilm formation.

scholarly journals Transmission of Respiratory Syncytial Virus Infection Within Families

2015 ◽  
Vol 2 (1) ◽  
Author(s):  
Terho Heikkinen ◽  
Heikki Valkonen ◽  
Matti Waris ◽  
Olli Ruuskanen
Keyword(s):  
Respiratory Syncytial Virus ◽  
Virus Infection ◽  
Respiratory Symptoms ◽  
Family Members ◽  
Primary Case ◽  
The Family ◽  
Nasal Swabs ◽  
Rsv Infection ◽  
Syncytial Virus ◽  
A Prospective Study

Abstract Background.  Because the production of an effective respiratory syncytial virus (RSV) vaccine for infants is challenging, vaccination of other family members is one viable alternative to prevent severe RSV illnesses in infants. Methods.  In a prospective study, we enrolled all family members of children who were hospitalized with RSV infection. Nasal swabs for RSV detection were obtained from all participating family members. Data on respiratory symptoms in the family members prior to and after the child's admission were collected using standardized questionnaires. Results.  At the time of or within 1 week after the index child's hospitalization, RSV was detected in 40 (77%) of the 52 families and in 60 (47%) of 129 family members. Forty-nine (82%) of RSV detections in the family members were associated with respiratory symptoms. A sibling or a parent was the probable primary case of RSV in 30 (58%) families. Respiratory syncytial virus loads in the nasal swabs were significantly higher (107.7) in index children than in their parents (105.1, P < .0001). Conclusions.  In most cases, the likely source of an infant's RSV infection is an older sibling or a parent. These findings support the strategy of reducing the burden of RSV in infants by vaccination of their family members.

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