Immunological relationships during primary infection with Heligmosomoides polygyrus (Nematospiroides dubius): H-2 linked genes determine worm survival

Parasitology ◽  
1991 ◽  
Vol 103 (1) ◽  
pp. 157-164 ◽  
Author(s):  
J. M. Behnke ◽  
F. N. Wahid
Keyword(s):  
Primary Infection ◽  
Congenic Mouse ◽  
Mouse Strains ◽  
Gene Products ◽  
Heligmosomoides Polygyrus ◽  
Duration Of Infection ◽  
Response Phenotype ◽  
Nematospiroides Dubius ◽  
Resistance To Infection ◽  
Congenic Mouse Strains

The course of primary infection was studied in BALB and B10 H-2 congenic mouse strains. The duration of infection, as assessed with regular faecal egg counts and worm burdens, was shorter in mice carrying the H-2s, H-2d or H-2q haplotypes when compared to mice with H-2b. Strains with H-2k were intermediate. An experiment was carried out to test the hypothesis proposed by Wassom, Krco & David (1987) predicting that the progeny of I–E+ve mouse strains crossed with I–E-ve strains, would show susceptibility rather than resistance to infection. This hypothesis was not substantiated by our data and we conclude that it does not apply to primary infections with Heligmosomoides polygyrus. It is proposed that the gene products of at least two loci within the H-2 (associated with the H-2b and H-2k haplotypes) are crucial in determining the response phenotype of mice to primary infection with H. polygyrus. One allele, (associated with the H-2b haplotype) may be preferentially affected by parasite-mediated immunosuppression.

Immunological realtionships during primary infection with Heligmosomides polygyrus (Nematospiroides dubius): expulsion of adult worms from fast responder syngeneic and hybrid strains of mice

Parasitology ◽  
1989 ◽  
Vol 98 (3) ◽  
pp. 459-469 ◽  
Author(s):  
F. N. Wahid ◽  
M. Robinson ◽  
J. M Behnke
Keyword(s):  
High Intensity ◽  
Primary Infection ◽  
Time Course ◽  
The Other ◽  
Mouse Strains ◽  
Heligmosomoides Polygyrus ◽  
Worm Expulsion ◽  
Nematospiroides Dubius ◽  
The One ◽  
Identical Gene

SUMMARYThe time-course of low and high intensity primary infections with Heligmosomoides polygyrus was monitored in SJL and SWR mice, both of which usually expel worms within 7 weeks of larval administration. Worm expulsion in these strains was not dependent on the intensity of infection, with low and high intensity worm burdens being lost within the same period of time. The ability to expel worms rapidly was inherited in a dominant manner in F1 offspring of SJL or SWR mice mated with C57Bl10 mice; the latter being a strain in which no loss of worms was evident within 10 weeks of infection. However, neither (SJL × C57Bl10)F1 nor (SWR × C57Bl10)F1 mice expelled worms as rapidly as the parental SJL and SWR strains. (SWR × B10G)F1 [H-2q] mice eliminated worms faster than (SWR × C57Bl10)F1 [H-2bq], suggesting that the b haplotype had a moderating influence on the expulsion process. In fact (SWR × B10G)F1 mice showed a significant reduction in worm burdens by week 4 but by weeks 6–8 the rate of worm loss had slowed considerably. In contrast, SJL and SWR mice, whilst initiating rejection slightly later, (after week 4) expelled all worms within the following 2 weeks. Thus two distinct patterns of response were observed among the fast responder strains as exemplified by SWR and SJL mice on the one hand and (SWR × B10G)F1 on the other. Our results support the hypothesis that the course of a primary infection with H. polygyrus is influenced by multiple host gene loci, some of which are encoded within the MHC. SJL and SWR mice probably have similar if not identical gene combinations at loci which determine a fast responder phenotype, distinguishing them from the other mouse strains which have been studied.

scholarly journals Immunological relationships during primary infection with Heligmosomoides polygyrus (Nematospiroides dubius): the capacity of adult worms to survive following transplantation to recipient mice

Parasitology ◽  
1987 ◽  
Vol 95 (3) ◽  
pp. 569-581 ◽  
Author(s):  
J. M. Behnke ◽  
Diane J. Williams ◽  
J. Hannah ◽  
D. I. Pritchard
Keyword(s):  
Host Resistance ◽  
Post Infection ◽  
Primary Infection ◽  
Worm Burden ◽  
Egg Production ◽  
Heligmosomoides Polygyrus ◽  
Duration Of Infection ◽  
Nematospiroides Dubius ◽  
Single Worm

SUMMARYChronic primary infections with Heligmosomoides polygyrus (Nematospiroides dubius) are still relatively poorly documented, particularly in relation to the role of host resistance in limiting worm survival. In the present work the duration of infection with H. polygyrus was studied in CFLP mice given doses of infective larvae ranging from 50 to 500 L3. The least heavily infected (50 L3) group ceased egg production earliest (week 36) whereas eggs were still detected in the faeces of mice given 500 larvae in week 42. At autopsy (week 42) mice given 50 larvae had virtually lost their entire worm burden with 5 out of 11 mice still harbouring a single worm each. However, all the mice in the group given 500 larvae were still infected, the highest worm burden being 93. The concentration of serum IgGl and specific antibody was highest in mice given 500 larvae, but sera taken from mice with declining worm burdens 19–38 weeks post-infection did not contain detectable host-protective antibody. During the course of infection in CFLP mice, H, polygyrus sustained irreversible changes in its capacity for subsequent survival. Thus, adult worms transferred to naive mice 2, 7, 14, 30 or 36 weeks post-infection did not live longer than worms of a comparable age in the respective donor group. In contrast, primary infection worms taken from jirds in which expulsion is usually completed by 6 weeks post-infection, re-established in mice and survived considerably longer than in the group of donor jirds. These results were discussed in relation to the possible interactions between parasite senility and immunomodulation, and host resistance in limiting primary infections with H. polygyrus in mice and jirds.

scholarly journals The relative involvement of Th1 and Th2 associated immune responses in the expulsion of a primary infection of Heligmosomoides polygyrus in mice of differing response phenotype

2003 ◽  
Vol 77 (2) ◽  
pp. 133-146 ◽  
Author(s):  
A. Ben-Smith ◽  
D.A. Lammas ◽  
J.M. Behnke
Keyword(s):  
Nitric Oxide ◽  
Primary Infection ◽  
Gastrointestinal Nematode ◽  
Inbred Strains ◽  
Specific Ige ◽  
Mouse Strains ◽  
Chronic Infections ◽  
Heligmosomoides Polygyrus ◽  
Response Phenotype ◽  
Th1 And Th2

AbstractT helper cell (Th1 and Th2) associated responses were examined following a primary infection with the gastrointestinal nematode Heligmosomoides polygyrus in five inbred strains of mice with different resistance phenotypes. Levels of (i) mast cell protease, (ii) specific IgE, (iii) nitric oxide and (iv) specific IgG2a, as markers of Th2 and Th1 associated responses, respectively, were determined in sera and intestinal fluids and correlated with worm burdens. The ‘fast’ responder (resistant) strains SWR and SJL produced strong Th2 and Th1 associated responses respectively in a mutually exclusive fashion. The F1 hybrid (SWR×SJL) F1, showed rapid expulsion of the parasite and expressed both intense Th1 and Th2 responses, suggesting synergism between Th1 and Th2 activity in these mice. The results indicate that both Th2 and Th1 responses operate in mice following a primary infection with H. polygyrus and that each Th response may be involved to a greater or lesser degree within certain strains. Resistance to H. polygyrus was found to correlate only to the intensity of either the gut-associated mastocytosis or nitric oxide production in these strains but not to either specific IgE or IgG2a titres. Chronic infections in the ‘slow’ response phenotype mouse strains CBA and C57BL/10, were associated with both poor Th2 and poor Th1-associated responses attributed to a general parasite-mediated immunosuppression of the host immune response to infection.

scholarly journals Erbb is linked to the alpha-globin locus on mouse chromosome 11.

1985 ◽  
Vol 5 (7) ◽  
pp. 1784-1786 ◽  
Author(s):  
J Silver ◽  
J B Whitney ◽  
C Kozak ◽  
G Hollis ◽  
I Kirsch
Keyword(s):  
Mouse Chromosome ◽  
Human Gene ◽  
Congenic Mouse ◽  
Mouse Strains ◽  
Somatic Cell Hybrids ◽  
Chromosome 11 ◽  
Homologous Sequences ◽  
Alpha Globin ◽  
Mouse Chromosome 11 ◽  
Congenic Mouse Strains

A fragment of the human gene for c-erb-B was used to map homologous sequences in mice. Analysis of somatic cell hybrids and recombinant inbred and congenic mouse strains indicated that this gene, designated Erbb, is closely linked to the gene for alpha-globin on mouse chromosome 11. Several genes controlling hematopoietic differentiation map to mouse chromosome 11.

Mating preferences of F2 segregants of crosses between MHC-congenic mouse strains

1978 ◽  
Vol 6 (1) ◽  
pp. 253-259 ◽  
Author(s):  
K. Yamazaki ◽  
M. Yamaguchi ◽  
P. W. Andrews ◽  
B. Peake ◽  
E. A. Boyse
Keyword(s):  
Congenic Mouse ◽  
Mouse Strains ◽  
Mating Preferences ◽  
Congenic Mouse Strains

scholarly journals Immunological relationships during primary infection with Heligmosomoides polygyrus. Regulation of fast response phenotype by H-2 and non-H-2 genes

Parasitology ◽  
1993 ◽  
Vol 107 (3) ◽  
pp. 343-350 ◽  
Author(s):  
F. N. Wahid ◽  
J. M. Behnke
Keyword(s):  
Fast Response ◽  
Antibody Responses ◽  
Mouse Strains ◽  
Hybrid Progeny ◽  
Heligmosomoides Polygyrus ◽  
Response Phenotype ◽  
Mhc Haplotype ◽  
Slow Responder ◽  
Dominant Genes ◽  
Responder Mouse

SummaryThe inheritance of response phenotype to Heligmosomoides polygyrus was investigated in Fl hybrid progeny of fast and slow responder mouse strains. The fast responses of SJL(H-2s) and SWR(H-2q) mice were mediated by dominant genes complementing each other in Fl hybrids which lost worms earlier and produced faster parasite-specific IgG1 antibody responses than either parent. However, the response of Fl hybrids from crosses of C57BL/10 (B10, H-2b) mice with either SJL or SWR differed from that of the parental strains and from each other: (B10 × SWR)F1 lost worms earlier than SWR whilst (B10 × SJL)F1 lost worms later than SJL mice. The Fl progeny of SJL mice with congenic strains B10.G (H-2q) and B10.S (H-2s) lost worms as quickly as SJL. Therefore, the response phenotype mediated by the genome of SJL mice was unaffected by H-2 heterozygosity (with H-2q) or homozygosity (H-2s) despite heterozygosity with B10 background genes, but was slowed significantly by heterozygosity with H-2b. All hybrids involving heterozygosity with B10, irrespective of MHC haplotype or background, failed to clear worms completely, in each case a proportion of mice harbouring residual worm burdens after loss of worms from parental strains.

Development of intra-natural killer complex (NKC) recombinant and congenic mouse strains for mapping and functional analysis of NK cell regulatory loci

1999 ◽  
Vol 49 (3) ◽  
pp. 238-241 ◽  
Author(s):  
A. A. Scalzo ◽  
Michael G. Brown ◽  
Dortha T. Chu ◽  
Jonathan W. Heusel ◽  
Wayne M. Yokoyama ◽  
...  
Keyword(s):  
Functional Analysis ◽  
Natural Killer ◽  
Nk Cell ◽  
Congenic Mouse ◽  
Mouse Strains ◽  
Regulatory Loci ◽  
Congenic Mouse Strains
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