The molecular evidence of Babesia microti infection in small mammals collected in Slovenia

Parasitology ◽  
2003 ◽  
Vol 126 (2) ◽  
pp. 113-117 ◽  
Author(s):  
D. DUH ◽  
M. PETROVEC ◽  
T. TRILAR ◽  
T. AVSIC-ZUPANC
Keyword(s):  
Small Mammals ◽  
Small Subunit ◽  
Clethrionomys Glareolus ◽  
Babesia Microti ◽  
Molecular Evidence ◽  
Rrna Gene ◽  
Small Subunit Rrna ◽  
Pcr Assay ◽  
Small Subunit Rrna Gene ◽  
Yellow Necked Mouse

In Europe, the zoonotic cycle of Babesia microti has not been determined so far. Recently, B. microti was detected in Ixodes ricinus ticks in Slovenia by using molecular methods. In order to investigate the mammalian hosts of B. microti in Slovenia we collected 261 small mammals representing 11 species. They were tested for the presence of babesial parasites with a PCR assay based on the nuclear small subunit rRNA gene (nss-rDNA). The bank vole (Clethrionomys glareolus) and yellow-necked mouse (Apodemus flavicollis) were infected with B. microti. The prevalence rate was 15·9% for C. glareolus and 11·8% for A. flavicollis. Nucleotide sequences of amplified portions of B. microti nss-rDNA from C. glareolus and A. flavicollis were indistinguishable from each other and identical with those previously described in I. ricinus ticks collected in Slovenia. The results of this study represent molecular evidence of B. microti in small mammals in Europe.

Detection of Kobe-typeBabesia microtiassociated with Japanese human babesiosis in field rodents in central Taiwan and southeastern mainland China

Parasitology ◽  
2008 ◽  
Vol 135 (6) ◽  
pp. 691-699 ◽  
Author(s):  
A. SAITO-ITO ◽  
N. TAKADA ◽  
F. ISHIGURO ◽  
H. FUJITA ◽  
Y. YANO ◽  
...  
Keyword(s):  
Mainland China ◽  
Small Subunit ◽  
Human Infection ◽  
Babesia Microti ◽  
Rrna Gene ◽  
Microscopical Examination ◽  
Small Subunit Rrna ◽  
Central Taiwan ◽  
Field Rodents ◽  
Human Babesiosis

SUMMARYField rodent surveys forBabesiainfection were performed from 2002 to 2005 in the vicinities of human babesiosis occurrences in Taiwan and mainland China.Babesia microtiwas identified by microscopical examination and/or PCR in 1Rattus coxingaand 1Crocidura horsfieldiiin central Taiwan and in 13Niviventer confucianusand 1Apodemus agrariusin Zhejiang and Fujian Provinces of southeastern China. Of 15B. microtisamples detected by PCR, all except 1 were shown to be the Kobe-type, the aetiological small subunit rRNA gene-type of the first Japanese patient; the exception was also a Kobe-related type. The Kobe-type had been found in rodents only in a few places including the human infection occurrence place in Japan. The internal transcribed spacer 1 to 2 sequences of the Taiwanese and Chinese Kobe-types were very similar to each other but considerably different (approx. 94% pairwise identities) from that of the Japanese Kobe-type. A Taiwanese Kobe-type strain was serologically differentiated from the Kobe strain originating from the Japanese first patient. The distribution of the Kobe-type in the vicinities of human babesiosis occurrences in Taiwan and China as well as in Japan is suggestive of involvement of the Kobe-type in Asian human babesiosis.

Phylogenetic analysis of Blastocystis isolates from different hosts based on the comparison of small-subunit rRNA gene sequences

2003 ◽  
Vol 126 (1) ◽  
pp. 119-123 ◽  
Author(s):  
Christophe Noël ◽  
Corinne Peyronnet ◽  
Delphine Gerbod ◽  
Virginia P Edgcomb ◽  
Pilar Delgado-Viscogliosi ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Small Subunit ◽  
Rrna Gene ◽  
Small Subunit Rrna ◽  
Gene Sequences ◽  
Small Subunit Rrna Gene

Genetic interrelationships of proteolyticClostridium botulinum types A, B, and F and other members of theClostridium botulinum complex as revealed by small-subunit rRNA gene sequences

1994 ◽  
Vol 64 (3-4) ◽  
pp. 273-283 ◽  
Author(s):  
R. A. Hutson ◽  
D. E. Thompson ◽  
P. A. Lawson ◽  
R. P. Schocken-Itturino ◽  
E. C. B�ttger ◽  
...  
Keyword(s):  
Small Subunit ◽  
Rrna Gene ◽  
Small Subunit Rrna ◽  
Gene Sequences ◽  
Small Subunit Rrna Gene

Free-living nematodes associated with pine cones of Pinus thunbergii and P. taeda at Japanese coastal and inland forest sites

Nematology ◽  
2019 ◽  
Vol 21 (4) ◽  
pp. 389-400 ◽  
Author(s):  
Yudai Kitagami ◽  
Natsumi Kanzaki ◽  
Toko Tanikawa ◽  
Yosuke Matsuda
Keyword(s):  
Gene Sequence ◽  
Small Subunit ◽  
Pinus Thunbergii ◽  
Rrna Gene ◽  
Small Subunit Rrna ◽  
Free Living ◽  
Small Subunit Rrna Gene ◽  
Forest Sites ◽  
High Bootstrap ◽  
Pine Cones

Summary We surveyed the distribution of nematodes in 56 cones of Pinus thunbergii collected from both live branches and on the forest floor in three coastal and inland habitats and in 11 cones of P. taeda collected at different heights. We identified 47 nematodes to family or genera by analysis of an 18S small subunit rRNA gene sequence. The frequencies of occurrence of free-living cone nematodes were 97% in coastal P. thunbergii, 92% in inland P. thunbergii, and 82% in P. taeda. Phylogenetic analysis assigned the nematodes to four clades with high bootstrap values. Nine sequences that were found only in cones on live branches were clustered with Panagrobelus stammeri and an unknown Panagrobelus sp. Our results imply that nematodes are commonly associated with cones in pine forest ecosystems and that a capacity for anhydrobiosis may be a key to surviving above-ground.

scholarly journals Description of Eurystomatella sinica n. gen., n. sp., with establishment of a new family Eurystomatellidae n. fam. (Protista, Ciliophora, Scuticociliatia) and analyses of its phylogeny inferred from sequences of the small-subunit rRNA gene

2010 ◽  
Vol 60 (2) ◽  
pp. 460-468 ◽  
Author(s):  
Miao Miao ◽  
Yangang Wang ◽  
Weibo Song ◽  
John C. Clamp ◽  
Khaled A. S. Al-Rasheid
Keyword(s):  
New Genus ◽  
Phylogenetic Analyses ◽  
Sister Group ◽  
Small Subunit ◽  
Rrna Gene ◽  
Small Subunit Rrna ◽  
New Family ◽  
Small Subunit Rrna Gene ◽  
The Family ◽  
Great Divergence

Recently, an undescribed marine ciliate was isolated from China. Investigation of its morphology and infraciliature revealed it as an undescribed species representing a new genus, Eurystomatella n. gen., the type of the new family Eurystomatellidae n. fam. The new family is defined by close-set, apically positioned oral membranelles and a dominant buccal field that is surrounded by an almost completely circular paroral membrane. The new genus is defined by having a small oral membranelle 1 (M1), bipartite M2 and well-developed M3, a body surface faintly sculptured with a silverline system in a quadrangular, reticulate pattern and a cytostome located at the anterior third of a large buccal field. The type species of the new genus, Eurystomatella sinica n. sp., is a morphologically unique form that is defined mainly by the combination of a conspicuously flattened body, several caudal cilia, extremely long cilia associated with the buccal apparatus and a contractile vacuole located subcaudally. According to phylogenetic analyses of small-subunit (SSU) rRNA gene sequences, Eurystomatella clusters with the genus Cyclidium, as a sister group to the family Pleuronematidae. The great divergence in both buccal and somatic ciliature between Eurystomatella and all other known scuticociliates supports the establishment of a new family for Eurystomatella.

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