Infection of Mammary Glands of Small Mammals in Eastern North America by Helminths

To determine whether small mammals living in natural settings harbor helminth infections in their mammary glands, we conducted a survey of helminths infecting rodents and soricimorphs in three widespread locations in the eastern United States: states of New York, Tennessee, and Georgia. We examined all the primary organs in all hosts, and identified all helminths. We also excised the complete mammary glands within their subcutaneous fat pads, then stained and mounted each whole mammary gland set for microscopical examination. A total of 53 individual hosts were examined, including 32 Peromyscus spp., 11 Mus musculus, 5 Sigmodon hispidus, 4 Clethrionomys gapperi, and 1 Blarina carolinensis. Helminths collected included Heligmosomoides sp., Hymenolepisdiminuta, Hymenolepis nana, Pterygodermatites peromysci, Schistosomatium douthitti, Syphacia obvelata, Syphacia sigmodontis, and Trichostrongylus sigmodontis. Four S. hispidus were infected by T. sigmodontis in the small intestine; in all four, we also found nematode larvae in lactiferous duct lumen and lactogenic tissue of the mammary glands. We were unable to identify the species of nematode larvae, but the co-occurrence with T. sigmodontis in all cases may suggest an association. Future studies should seek to identify such larvae using molecular and other methods, and to determine the role of these mammary nematode larvae in the life cycle of the identified species. No other host species harbored helminths in the mammary glands. Overall, our results suggest that mammary infections in wild small mammals are not common, but warrant inclusion in future surveys.

Some Physical Processes Involved in Deformation and Fracture

<p>The effects of strain rate, of moisture content, and of tracheid structure on the transverse fracture properties of Pinus Radiata have been studied. Small rectangular blocks were loaded to failure in transverse tension, with the conditions of fracture being varied as follows: (i) strain raite - at 2 x 10 caret-6 sec caret-1, and from 10 caret-5 to 10 caret 2 sec caret-1 in decade steps , (ii) moisture content - airdry (12.7%) and saturated, and (iii) structure - springwood and summerwood. Microscopical examination (both scanning electron and optical) of the surfaces produced by the facture showed that the cellular level, either of two types of failure could occur. These are called transwall and intrawall; transwall is the longitudinal splitting of a tracheid wall, and intrawall is a splitting between adjacent tracheids.</p>

Some Physical Processes Involved in Deformation and Fracture

<p>The effects of strain rate, of moisture content, and of tracheid structure on the transverse fracture properties of Pinus Radiata have been studied. Small rectangular blocks were loaded to failure in transverse tension, with the conditions of fracture being varied as follows: (i) strain raite - at 2 x 10 caret-6 sec caret-1, and from 10 caret-5 to 10 caret 2 sec caret-1 in decade steps , (ii) moisture content - airdry (12.7%) and saturated, and (iii) structure - springwood and summerwood. Microscopical examination (both scanning electron and optical) of the surfaces produced by the facture showed that the cellular level, either of two types of failure could occur. These are called transwall and intrawall; transwall is the longitudinal splitting of a tracheid wall, and intrawall is a splitting between adjacent tracheids.</p>

Prevalence of gastrointestinal endoparasites in Waringin sheep (Ovis aries) fed with complete forage and forage-concentrate

Feed composition has a substantial impact on the persistence of gastrointestinal endoparasite infection in sheep. This study aimed to compare the prevalence of gastrointestinal endoparasites in Waringin sheep (Ovis aries) fed with complete forage and feed concentrate in Sidomulyo Village, Langkat Regency, North Sumatra. The study has been conducted from June to August 2020 with a total of 20 sheep for each type of feed. Fecal samples from each sheep were prepared for microscopical examination using sedimentation technique (glass beads). Gastrointestinal endoparasites was identifiedmorphologically. The results documented 3 genera of endoparasites namely Haemonchus (Nematoda), Fasciola (Trematoda) and Paramphistomum (Trematoda). Based on the type of forages, sheep fed with forage-concentrate showed a lower number of helminth eggs than the complete forage group. Haemonchus sp was identified as the most frequent parasite in both type of feeds. The intensity was categorized from mild-to-moderate level of infection. Provision of forage-concentrate was then proven to prevent a high rate of infection in Waringin sheep.

Endoparasite infestation of Indian Muntjac (Muntiacus muntjak) in Citra Pesona Ladangku Animal Park, North Sumatra

An investigation on the infestation by endoparasites in the fecal samples of Indian Muntjac (Muntiacus muntjak) captived at Citra Pesona Ladangku Animal Park, North Sumatera, Indonesia had been conducted from October to November 2020. The study aimed to determine the endoparasite species, prevalence, and intensity of the infestation. Fecal samples were prepared for microscopical examination using glass bead sedimentation method. Based the observation on 9 deers, three species of endoparasites were found namely Ascaris sp, Haemonchus sp and Paramphistomum sp. Observations in the first week revealed the prevalence of each species from often (Ascaris sp, Haemonchus sp) to common (Paramphistomum sp) with light (Haemonchus sp, Paramphistomum sp) to moderate (Ascaris sp). Observations in the second week only revealed the presence of Paramphistomum with often prevalency and light intensity of infection.

STUDY ON SOME ASPECTS OF PATHOGENESIS OF RHODOCOCCUS EQUI IN MICE

In order to study some aspects of pathogenesis of Rhodococcus equi, 60 white Swiss mice were used. They were randomly divided into 2 groups. The 1s' group (40 animals) was inoculated subcutaneously with a dose of 4x10° CFU .of R.equi while the 2nd group was kept as a control. Four animals from the 1st group and 2 animals from the 2nd group were sacrified at day, 1,2,4,8,12,16,20,24,28 and 30 postinoculation (P.I) Six animals of 1stgroup were died during the first and second days P.I. Microscopical examination showed acute to subacute suppurative inflammation in the liver, lung and spleen at I to 4 days PI. While pyogranulomatous lesions and epithelioid granuloma were seen in liver, lungs and spleen at 4 to 24 days PI R.equi was isolated liver, lungs, spleen, kidney and brain in the first day PI. The bacteria persisted in the spleen from and brain up to 20th day, in the liver up to 24" day while in the lungs, and kidney bacterial isolation continuous till 28th day PI.

Intestinal coccidiosis (Apicomplexa: Eimeriidae) in a Himalayan Griffon Vulture Gyps himalayensis

A free-ranging juvenile Himalayan Griffon Vulture from the Haldwani forest range division, Nainital, Uttarakhand, on detailed inspection, showed dullness, weakness, emaciation, ruffled feathers, droopy head, and watery whitish diarrhoea. It was unable to bear its own weight on both the legs. The bird died later and the post mortem revealed haemorrhagic intestinal tract. Based on the findings and microscopical examination it was confirmed as intestinal coccidiosis.

Effect of Cadmium Chloride on the Histological Structure of Lung in Adult Male Mice with and without Parsley Oil

This study is to detect the toxic effect of cadmium chloride on the histological structure of the lung and the effect of parsley oil to amilorate these changes In this experiement 40 adult male mice were divided into four groups. Goup A (control group) in this group animals were injected with the normal saline intraperitoneally single daily dose for 30 days. Group B injected intrapertioneally with cadmium chloride single daily dose 3.5 mg/kg body weight for 30 days. Group C injected intraperetonially with cadmium chloride in a dose of 3.5 mg/kg body weight. Intragastric tube was put to recieve parsley oil in a dose of 0.5 ml/kg body weight prior to cadmium injection. The two drugs were given for 30 days. Group D recieved 0.5 ml/kg body weight by intragastric tube of parsley oil for 30 days. At the end of this experiement, the animals were sacrified the lungs were collected from all groups and prepared for light microscopical examination. Histological changes were detected in cadmium chloride treated group in comparison with the control group including congestion, inflammatory cell infiltration, interstial pneumonia (decreased alveolar space), thickening of interalveolar septum and damge to the alveolar cells. All these changes were eliminated by giving parsley oil.

The Efficiency of Hand Sanitizers Against Microbes From Coins

Objective:This study aimed to examine the effectiveness of hand sanitizers against bacteria from circulating coins during COVID 19 era.Method:22-coin samples have been collected by the research group from taxi drivers in Nablus city, Then the samples were transferred in a sterile plastic bag to the Microorganism Laboratory (17B1140) at the Faculty of Medicine and Health Sciences at An-Najah National University. The collected coins were swabbed with normal saline and cultured on nutrient agar for enrichment. After their appearance, the grown colony has been sub-cultured on Blood, MacConkey, and Uri select, for identification with the assistance of microscopical examination and chemical tests for confirmation. To answer the question of the study, four different sanitizers have been applied to measure their antimicrobial activity against microbes from circulating metal coins. Result:The results showed that sanitizer A (Hand sanitizer gel) (ג'ל להיגיינת הידיים ללא צורך , בשטיפה ובמים) is the most effective one of the four sanitizers that were used, followed by sanitizer (D)(HiGeen )(JO), then sanitizer (C)(National ) (PS), while sanitizer (B)(Icona London) (UK) was the least effective one.Conclusion:The research group have conducted research on coins circulated from taxi drivers during COVID 19 era, hence the number of isolated bacteria was low, due to the current sterilization and prevention behaviors like the using of sanitizers containing 70% of alcohol, which was the best among the used types in the current study as well as it has been observed that excessive use of alcohol- based hand sanitizers can cause microorganisms resistant and or less affected to repeated use of hand sanitizer. Hence, Hand sanitizers may need to be used appropriately in combination with other procedures.

Contrast-Enhanced Tissue Processing of Fibrillin-Rich Elastic Fibres for 3D Visualization by Volume Scanning Electron Microscopy

Elastic fibres constitute an important component of the extracellular matrix and currently are the subject of intensive study in order to elucidate their assembly, function and involvement in cell–matrix interactions and disease. However, few studies to date have investigated the 3D architecture of the elastic fibre system in bulk tissue. We describe a protocol for preparation of tissue samples, including primary fixation and backscatter electron contrast-enhancement steps, through dehydration into stable resin-embedded blocks for volume electron microscopy. The use of low molecular weight tannic acid and alcoholic lead staining are critical stages in this procedure. Block preparation by ultramicrotomy and evaporative metal coating prior to microscopical examination are also described. We present images acquired from serial block face scanning electron microscopy of cornea and aorta showing target structures clearly differentiated from cells and other matrix components. The processing method imparts high contrast to fibrillin-containing elastic fibres, thus facilitating their segmentation and rendering into 3D reconstructions by image analysis software from large serial image datasets.