Ultrastructural and molecular characterization of Bacillidium vesiculoformis n. sp. (Microspora: Mrazekiidae) in the freshwater oligochaete Nais simplex (Oligochaeta: Naididae)

Parasitology ◽  
2004 ◽  
Vol 130 (1) ◽  
pp. 31-40 ◽  
Author(s):  
D. J. MORRIS ◽  
R. S. TERRY ◽  
K. B. FERGUSON ◽  
J. E. SMITH ◽  
A. ADAMS
Keyword(s):  
Phylogenetic Analysis ◽  
New Species ◽  
Infected Cell ◽  
Host Cell ◽  
Developmental Stages ◽  
Parasite Development ◽  
Adhesive Quality ◽  
Transmission Studies ◽  
A New Species

The development of a new species, Bacillidium vesiculoformis n. sp. (Microspora, Mrazekiidae), is described from the freshwater oligochaete Nais simplex (Oligochaeta, Naididae). Initial stages of parasite development consist of a monokaryotic merogony within a haemocyte of the intestinal blood sinus. The resulting hypertrophied haemocyte is attached to the chloragocytes of the sinus by fine cytoplasmic extensions with the sinus around the cell becoming greatly enlarged. The meronts within the haemocyte form diplokaryotic sporonts that undergo sporogenesis directly within the cytoplasm of the host cell. The infected cell becomes packed with spores and developmental stages, causing it dramatically to increase in size, eventually rupturing the oligochaete and cell. Sporogony appears to be disporoblastic. Released spores were observed to have an adhesive quality. Transmission studies conducted with mature spores failed to transmit the parasite horizontally although vertical transmission was observed. Phylogenetic analysis of the parasite demonstrated that B. vesiculoformis clustered with microsporidian parasites of bryozoa and two other microsporidians, Janacekia debaiseuxi and an unidentified Bacillidium sp.

scholarly journals Discovery and Characterization of a Novel Ampelovirus on Firespike

Viruses ◽  
2020 ◽  
Vol 12 (12) ◽  
pp. 1452
Author(s):  
Yaqin Wang ◽  
Yu Song ◽  
Yongzhi Wang ◽  
Mengji Cao ◽  
Tao Hu ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
New Species ◽  
Nicotiana Benthamiana ◽  
Rna Virus ◽  
Genomic Rna ◽  
Sequence Comparisons ◽  
Molecular Features ◽  
Viral Genomic Rna ◽  
A New Species

A novel RNA virus was identified in firespike (Odontonema tubaeforme) plants exhibiting leaf curling and chlorosis. The molecular features of the viral genomic RNA and proteins resemble those of ampeloviruses. Based on sequence comparisons and phylogenetic analysis, we propose a new species in the genus Ampelovirus, which we have tentatively named Firespike leafroll-associated virus (FLRaV). Bioassays showed that the virus is mechanically transmissible to Nicotiana benthamiana. In addition, a full-length cDNA clone of FLRaV could successfully infect N. benthamiana via agroinfiltration.

Culex (Culex) longitubus, A New Species of the Mimeticus Subgroup (Diptera: Culicidae) From Bhutan

2021 ◽  
Author(s):  
Pradya Somboon ◽  
Thanari Phanitchakun ◽  
Jassada Saingamsook ◽  
Rinzin Namgay ◽  
Ralph E Harbach
Keyword(s):  
Phylogenetic Analysis ◽  
New Species ◽  
Male Genitalia ◽  
Immature Stages ◽  
High Altitudes ◽  
Coi Sequences ◽  
Anal Papillae ◽  
A New Species

Abstract Culex longitubus Somboon, Namgay & Harbach is described as a new species of the Mimeticus Subgroup of the subgenus Culex. The larva is most similar to the larva of Cx. tianpingensis Chen from China, but is distinguished by the length of the siphon and the anal papillae, the form of the comb scales and pecten spines, and the development of setae 7-P, 13-T, 1-X, and 4-X. The adults have wing markings and male genitalia similar to those of species of the Mimeticus Complex. Phylogenetic analysis of COI sequences revealed that the new species is closely related to Cx. murrelli Lien of the Mimulus Complex. The immature stages of the new species were found in stagnant pools and marshes at high altitudes in several districts of Bhutan.

A new species of Cynomops (Chiroptera: Molossidae) from the northwestern slope of the Andes

Mammalia ◽  
2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Daniela Arenas-Viveros ◽  
Pamela Sánchez-Vendizú ◽  
Alan Giraldo ◽  
Jorge Salazar-Bravo
Keyword(s):  
Phylogenetic Analysis ◽  
New Species ◽  
Molecular Data ◽  
Mtdna Sequences ◽  
The Andes ◽  
Western Side ◽  
Morphological And Molecular Data ◽  
A New Species ◽  
Lines Of Evidence

Abstract The systematics and taxonomy of the broadly distributed bats of the genus Cynomops has changed considerably in the last few years. Among the major changes, Cynomops abrasus was split into two species of large-bodied forms (Cynomops mastivus and C. abrasus) distributed east of the Andes. However, large Colombian specimens identified as C. abrasus from the western side of the Andes had yet to be included in any revisionary work. Phylogenetic analysis performed in this study, using mtDNA sequences (Cytochrome-b), revealed that these Colombian individuals are more closely related to Cynomops greenhalli. Morphological and molecular data allowed us to recognize populations from western Colombia, western Ecuador and northwestern Peru, as members of a new species of Cynomops. Characters that allow for its differentiation from C. greenhalli include a larger forearm, paler but more uniform ventral pelage, more globular braincase, and well-developed zygomatic processes of the maxilla (almost reaching the postorbital constriction). This study serves as another example of the importance of including multiple lines of evidence in the recognition of a new species. Given its rarity and the advanced transformation of its habitat, this new species is particularly important from a conservation perspective.

Description of a new species of Auriculostoma (Digenea: Allocreadiidae) from Characidium heirmostigmata (Characiformes: Crenuchidae) from Argentina, using morphological and molecular data

2021 ◽  
Vol 95 ◽  
Author(s):  
M.M. Montes ◽  
J. Barneche ◽  
Y. Croci ◽  
D. Balcazar ◽  
A. Almirón ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
New Species ◽  
National Park ◽  
Molecular Data ◽  
Rrna Gene ◽  
28S Rrna ◽  
The Family ◽  
Parasitological Survey ◽  
Morphological And Molecular Data ◽  
A New Species

Abstract During a parasitological survey of fishes at Iguazu National Park, Argentina, specimens belonging to the allocreadiid genus Auriculostoma were collected from the intestine of Characidium heirmostigmata. The erection of the new species is based on a unique combination of morphological traits as well as on phylogenetic analysis. Auriculostoma guacurarii n. sp. resembles four congeneric species – Auriculostoma diagonale, Auriculostoma platense, Auriculostoma tica and Auriculostoma totonacapanensis – in having smooth and oblique testes, but can be distinguished by a combination of several morphological features, hosts association and geographic distribution. Morphologically, the new species can be distinguished from both A. diagonale and A. platense by the egg size (bigger in the first and smaller in the last); from A. tica by a shorter body length, the genital pore position and the extension of the caeca; and from A. totonacapanensis by the size of the oral and ventral sucker and the post-testicular space. Additionally, one specimen of Auriculostoma cf. stenopteri from the characid Charax stenopterus (Characiformes) from La Plata River, Argentina, was sampled and the partial 28S rRNA gene was sequenced. The phylogenetic analysis revealed that A. guacurarii n. sp. clustered with A. tica and these two as sister taxa to A. cf. stenopteri. The new species described herein is the tenth species in the genus and the first one parasitizing a member of the family Crenuchidae.

The genus Xylaria (Xylariaceae) in the south of China—6. A new Xylaria species based on morphological and molecular characters

Phytotaxa ◽  
2013 ◽  
Vol 147 (2) ◽  
pp. 48 ◽  
Author(s):  
HAI-XIA MA ◽  
LARISSA VASILYEVA ◽  
YU LI
Keyword(s):  
Phylogenetic Analysis ◽  
New Species ◽  
Its Sequences ◽  
Guizhou Province ◽  
Nrdna Its ◽  
Undescribed Species ◽  
The South ◽  
A New Species ◽  
Molecular Characters

Xylaria fusispora, an undescribed species of Xylaria (Xylariales, Xylariaceae), is described and illustrated as a new species based on collections from Guizhou Province, China. Both morphology and phylogenetic analysis of nrDNA ITS sequences support the establishment of this new species. The fungus is characterized by its fusoid-equilateral ascospores and an ascus apical ring not bluing in Melzer’s reagent. The differences between the new species and the related fungi are discussed.

Pilaira australis sp. nov. (Mucorales, Mucoromycota) isolated from emu faeces in Australia

Phytotaxa ◽  
2017 ◽  
Vol 329 (3) ◽  
pp. 277 ◽  
Author(s):  
ANDREW S. URQUHART ◽  
PAULINE M. L. COULON ◽  
ALEXANDER IDNURM
Keyword(s):  
Phylogenetic Analysis ◽  
New Species ◽  
Species Identification ◽  
Internal Transcribed Spacers ◽  
Molecular Phylogenetic Analysis ◽  
Unique Combination ◽  
Dromaius Novaehollandiae ◽  
Molecular Phylogenetic ◽  
Carbon Utilisation ◽  
A New Species

Pilaira australis, a new species of fungus in the coprophilous genus Pilaira, was isolated from emu (Dromaius novaehollandiae) faeces and is described. Morphologically, the species resembles other species in the genus, particularly P. moreaui, except differs in its unique combination of sporangiophore height and sporangiospore length. Molecular phylogenetic analysis indicates that P. australis is distinct from other species in the genus with two regions, the internal transcribed spacers (ITS) and a fragment of the pyrG gene, showing 91% and 90% identity to the nearest species, respectively. Ultrastructure features and carbon utilisation were determined for P. australis, and may provide characteristics for species identification in this genus.

Codonopsis atriplicifolia (Campanulaceae), a new species from western Sichuan, China

Phytotaxa ◽  
2021 ◽  
Vol 512 (3) ◽  
Author(s):  
YU-YANG ZHOU ◽  
YUN-HAO SI ◽  
ZHEN ZHANG ◽  
QIANG WANG ◽  
YAN YU
Keyword(s):  
Phylogenetic Analysis ◽  
New Species ◽  
Chloroplast Dna ◽  
Molecular Phylogenetic Analysis ◽  
Dna Fragments ◽  
Western Sichuan ◽  
Molecular Phylogenetic ◽  
A New Species

Codonopsis atriplicifolia (Campanulaceae) from western Sichuan, China, is described here as a new species, which is similar to C. subscaposa in a number of characters. The results of molecular phylogenetic analysis of 25 species of Codonopsis based on chloroplast DNA fragments (matK, petD with petB-petD, rbcL) show that C. atriplicifolia is closely related to C. farreri. In morphology, the new species is distinctly different from the allied species by its linear-lanceolate leaves with subentire to pinnatipartite margins.

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