Observations on Developing Blood Vessels in Rat Spinal Cord—A High Voltage Electron Microscopy Study

1974 ◽  
Vol 32 ◽  
pp. 66-67
Author(s):  
Richard S. Hannah
Keyword(s):  
Electron Microscopy ◽  
Spinal Cord ◽  
High Voltage ◽  
Microscopy Study ◽  
Electron Microscopy Study ◽  
High Voltage Electron Microscopy ◽  
Thin Sections ◽  
Voltage Electron ◽  
High Voltage Electron ◽  
Transmission Electron

The formation of junctional complexes between endothelial cell processes was examined in rat spinal cords, from age birth to six weeks. Segments of spinal cord were removed from the region of the cervical enlargement and fixed. For comparative purposes, animals from each time group were subdivided into groups, fixed by either immersion or perfusion with an aldehyde combination in sodium cacodylate buffer and embedded in Araldite. Thin sections were examined by conventional transmission electron microscopy. Thick sections (0.5μ - 1.0μ) were stained with uranyl magnesium acetate for four hours at 60°C and lead citrate for 30 mins. and examined in the AEI Mark II High Voltage Electron Microscope.

On the High Voltage Electron Microscopy (1 MeV) of Biological Thick Sections

1972 ◽  
Vol 30 ◽  
pp. 464-465
Author(s):  
William H. Massover
Keyword(s):  
Electron Microscopy ◽  
High Voltage ◽  
Present Report ◽  
Biological Tissues ◽  
Specimen Preparation ◽  
Technical Problems ◽  
High Voltage Electron Microscopy ◽  
Thin Sections ◽  
Voltage Electron ◽  
High Voltage Electron

Stereoscopic examination of thick sections of fixed and embedded biological tissues by high voltage electron microscopy has been shown to allow direct visualization of three-dimensional fine structure. The present report will consider the occurrence of some new technical problems in specimen preparation and Image interpretation that are not common during lower voltage studies of thin sections.Thick Sectioning and Tissue Coloration - Epon sections of 0.5 μm or more that are cut with glass knives do not have a uniform thickness as Judged by their interference colors; these colors change with time during their flotation on the knife bath, and again when drying onto the specimen support. Quoted thicknesses thus must be considered only as rough estimates unless measured in specific regions by other methods. Chloroform vapors do not always result in good spreading of thick sections; however, they will spread spontaneously to large degrees after resting on the flotation bath for several minutes. Ribbons of thick sections have been almost impossible to obtain.

scholarly journals The chondriome of selected trypanosomatids. A three-dimensional study based on serial thick sections and high voltage electron microscopy.

1975 ◽  
Vol 66 (2) ◽  
pp. 404-413 ◽  
Author(s):  
J J Paulin
Keyword(s):  
Electron Microscopy ◽  
High Voltage ◽  
Three Dimensional ◽  
Flagellar Pocket ◽  
High Voltage Electron Microscopy ◽  
Thin Sections ◽  
Voltage Electron ◽  
Trypomastigote Form ◽  
High Voltage Electron ◽  
Three Dimensional Models

The unitary nature of the chondriome of two species of trypanosomatids, Blastocrithidia culicis and Trypanosoma cruzi, has been demonstrated by utilizing serial thick-sectioning techniques combined with high voltage electron microscopy. Profiles of mitochondrial elements seen in thin sections and suspected to be parts of a continuum were confirmed by serial thick sectioning (0.25-0.50 mum thick) and stereopair analysis to be parts of the same mitochondrion. Three-dimensional models obtained from tracings of mitochondrial profiles on cellulose acetate reveal the mitochondrion of B. culicis to consist of a posterior mass with six tubular extensions extending upward and terminating in the anterior apex. The kinetoplast was found suspended between two of the tubular extensions, or less frequently, protuding as a nodule from one of the extensions. A bifurcation of one of the extensions was found in some specimens. The mitochondrion of T. cruzi consists of a triangular-shaped convoluted tubule, the base being the kinetoplast portion while the apex is directed posteriorly. The mitochondrion bifurcates behind the flagellar pocket, lateral to the kinetoplast, sending two entwined extensions into the tenuous anterior apex. Whether the mitochondrion of T. cruzi is unitary in the trypomastigote form was not determined in this study, since only epimastigote forms were used.

High Voltage Electron Microscopy of Muscle

1969 ◽  
Vol 27 ◽  
pp. 96-97
Author(s):  
Robert V. Rice ◽  
J. S. Lally
Keyword(s):  
Electron Microscopy ◽  
Fine Structure ◽  
High Voltage ◽  
Striated Muscle ◽  
High Voltage Electron Microscopy ◽  
Thin Sections ◽  
Voltage Electron ◽  
High Voltage Electron ◽  
Cell Biol ◽  
Adjacent Structures

Several structures have been proposed to account for the appearance of Z and M-lines seen in thin sections of striated muscle. The high penetrating power of 800,000 to 1,000,000 volt electrons coupled with stereology offers a unique opportunity to resolve the complicated fine structure of Z and M-lines. In addition use has been made of the recently developed extraction and reconstitution of Z and M-lines (Stromer, Hartshorne, Mueller, and Rice, J. Cell Biol., 40, 167, 1969). Removal of portions of these structures helps to eliminate confusion due to adjacent structures.

Histo-grade diamond knives may be an appropriate tool for high-voltage electron microscopy

1991 ◽  
Vol 49 ◽  
pp. 296-297
Author(s):  
M.E. Rock ◽  
J.A. Anderson ◽  
P.S. Binder
Keyword(s):  
Electron Microscopy ◽  
High Voltage ◽  
Stereo Pair ◽  
Specimen Thickness ◽  
Serial Sections ◽  
High Voltage Electron Microscopy ◽  
Thin Sections ◽  
Voltage Electron ◽  
High Voltage Electron ◽  
Diamond Knife

High voltage electron microscopy (HVEM) has been employed in various ways (whole mounts of cells stereo pair imaging, axial tomography, and serial sections for reconstruction) to elucidate three dimensional (3-D) ultrastructural data. The increased specimen thickness allows further data analysis unobtainable from ultra-thin sections. HVEM can reduce the number of sections needed in 3-D reconstructiortby approximately ten times over conventional transmission electron microscopy (CTEM). But increasing section thickness also increases wear on the diamond knife used to section. We have compared the serial sections obtained from a histo-grade diamond knife with those from an E.M. grade ultra-knife. Both sets of sections were cut 0.5 μm thick from the same block, and evaluated under the one million volt beam of the HVEM.

Sign in / Sign up

Export Citation Format

Share Document