Electron Cytochemical Study of Carbon Dioxide Laser Effect on Rhesus Monkey Cornea

1974 ◽  
Vol 32 ◽  
pp. 224-225
Author(s):  
K. C. Tsou ◽  
J. Morris ◽  
P. Shawaluk ◽  
B. Stuck ◽  
E. Beatrice
Keyword(s):  
Carbon Dioxide ◽  
Electron Microscope ◽  
Rhesus Monkey ◽  
Carbon Dioxide Laser ◽  
Cytochemical Study ◽  
Laser Effect

While much is known regarding the effect of lasers on the retina, little study has been done on the effect of lasers on cornea, because of the limitation of the size of the material. Using a combination of electron microscope and several newly developed cytochemical methods, the effect of laser can now be studied on eye for the purpose of correlating functional and morphological damage. The present paper illustrates such study with CO2 laser on Rhesus monkey.

Electron Cytochemical Study of Corneal Epithelial Repair After Carbon Dioxide Laser Damage

1976 ◽  
Vol 34 ◽  
pp. 100-101
Author(s):  
K. C. Tsou ◽  
J. Severdia ◽  
B. Stuck ◽  
E. Beatrice
Keyword(s):  
Electron Microscopy ◽  
Carbon Dioxide ◽  
Rhesus Monkey ◽  
Carbon Dioxide Laser ◽  
Laser Damage ◽  
Adjacent Area ◽  
Cytochemical Study ◽  
Corneal Epithelial ◽  
Cacodylate Buffer ◽  
Center Area

Investigation of laser damage to the eye relies mainly on electron microscopy because of the limitation of the amount of tissue available for such study and the low level of damage that is discernible only under the electron microscope (1). Previously, we showed that functional damage to Rhesus monkey cornea epithelium by carbon dioxide laser could be studied with cytochemical methods adaptable to electron microscopy (2). The present paper reports repair of corneal epithelium in Rhesus monkey, following treatment by carbon dioxide laser.Rhesus monkey corneas were divided into three sections: the damaged area in the center (Area 1), the adjacent area (Area 2), and the area next to the sclera region (Area 3). The tissues were fixed in 3% glutaraldehyde in 0.25 M sucrose overnight for NADH diaphorase (2) and Mg++-ATPase (3) study, and postosmicated with 1% Os04 in cacodylate buffer. Lasing effect could not be seen visually after 24 hrs.

Carbon dioxide laser effect on platelet function and surface ultrastructure in vitro

1988 ◽  
Vol 8 (3) ◽  
pp. 259-263 ◽  
Author(s):  
Michael Eldar ◽  
Dov Gal ◽  
Meir Djaldetti ◽  
Dan Douer ◽  
Ester Rosner ◽  
...  
Keyword(s):  
Carbon Dioxide ◽  
Platelet Function ◽  
Carbon Dioxide Laser ◽  
Surface Ultrastructure ◽  
Laser Effect

Lased Enamel: Ultrastructural Observations of Pulsed Carbon Dioxide Laser Effects

1972 ◽  
Vol 51 (2) ◽  
pp. 455-460 ◽  
Author(s):  
Ralph H. Stern ◽  
Johanna Vahl ◽  
Reidar F. Sognnaes
Keyword(s):  
Carbon Dioxide ◽  
Carbon Dioxide Laser ◽  
Ruby Laser ◽  
Electron Microscopic ◽  
Human Enamel ◽  
Scanning Electron Microscopic ◽  
Laser Effect ◽  
Scanning Electron ◽  
Surface Changes

Scanning electron microscopic observations of the pulsed carbon dioxide laser effect on human enamel support microradiographic findings and indicate that this laser is significantly more efficient than the ruby laser within the limits of this investigation. Surface changes which were suggestive of fusion occurred between energy densities of 13 to 50 joules per square centimeter.

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