Vesicular system associated with spermiogenesis of bullfrog

1987 ◽  
Vol 45 ◽  
pp. 818-819
Author(s):  
K. C. Liu ◽  
S. F. Tsay
Keyword(s):  
Electron Microscopy ◽  
Microscopic Study ◽  
Electron Microscopic Study ◽  
Reproductive System ◽  
Seminiferous Tubule ◽  
Rana Catesbeiana ◽  
Routine Procedure ◽  
Electron Microscopic ◽  
Twisted Tubules ◽  
Electron Lucent

In the histologic and electron microscopic study of the male reproductive system of bullfrog, Rana catesbeiana, a vesicular system associated with spermiogenesis was observed. It appeared in the lumenal space of the seminiferous tubule (Fig. 1), in the heads of spermatids (Fig. 2), associated with the chromatins of the spermatid (Fig. 4). As deduced from sections, this vesicular system consisted of vesicles of various size or a large group of waving and twisted tubules (Fig. 3), After routine procedure of treatment for electron microscopy, the lumens of both of the vesicles and tubules were electron lucent.In human, vesicles and vesicular system associated with reproductive cell and tissue were reported. In abnormal spermiogenesis, flower-like body, actually vesicles, and giant vesicle associated with the head of spermatid were observed. In both cases the number of vesicle was limited from a single one to a few.

scholarly journals Electron-microscopic study of measles virus in lymphocytes of affected children

1973 ◽  
Vol 71 (3) ◽  
pp. 447-451 ◽  
Author(s):  
H. K. Narang
Keyword(s):  
Electron Microscopy ◽  
Incubation Period ◽  
Microscopic Study ◽  
Cell Lines ◽  
Electron Microscopic Study ◽  
Inclusion Bodies ◽  
Measles Virus ◽  
Internal Diameter ◽  
Electron Microscopic ◽  
Different Types

SUMMARYTwo different types of inclusion bodies have been found by electron-microscopy in the cytoplasm of sectioned lymphocytes from children infected with measles.The first is tubular, 18 nm. internal diameter, embedded in osmiophilic material, and is found during the incubation period. It is not thought to be specific for measles, and is probably identical with structures recently reported in HEp-2 cell lines and tumour tissue.The second type is less obviously tubular and morphologically resembles measles nucleocapsid. This was found only after the rash had appeared.

scholarly journals ELECTRON MICROSCOPIC STUDY OF THE ATPASE ACTIVITY OF THE BAI STRAIN A (MYELOBLASTOSIS) AVIAN TUMOR VIRUS

1962 ◽  
Vol 15 (3) ◽  
pp. 451-462 ◽  
Author(s):  
Alex B. Novikoff ◽  
Guy de Thé ◽  
D. Beard ◽  
J. W. Beard
Keyword(s):  
Electron Microscopy ◽  
Plasma Membrane ◽  
Atpase Activity ◽  
Light Microscopy ◽  
Microscopic Study ◽  
Electron Microscopic Study ◽  
Frozen Sections ◽  
Electron Microscopic ◽  
Tumor Virus

Thymus glands of chicks with leukemia induced by BAI strain A (myeloblastosis) virus were fixed in cold 4 per cent formaldehyde-sucrose. Frozen sections were incubated in the ATPase medium of Wachstein and Meisel and studied by light microscopy and electron microscopy. The ATPase activity of the virus is localized to the outermost membrane of the virus. The membrane of the blast-like cells of the thymus cortex from which the virus emerges, by budding, also possesses such activity. It appears likely that the outermost membrane of the virus is derived from the plasma membrane of these cells.

An Electron Microscopic Study of Kidney Cells in the Lizard Treated with Mercuric Chloride

1982 ◽  
Vol 40 ◽  
pp. 60-61
Author(s):  
E. C. V. Ooi ◽  
E. C. Chew
Keyword(s):  
Electron Microscopy ◽  
Microscopic Study ◽  
Electron Microscopic Study ◽  
Mercuric Chloride ◽  
Kidney Cells ◽  
Electron Microscopic ◽  
Cellular Effects ◽  
Tissue Alterations

The nephrotoxicity of mercury is well established, especially in mammals and some fishes. However, no information on tissue alterations caused by mercurial compounds in reptiles is available. The present work was to study by using electron microscopy the cellular effects of mercury on kidney cells in the lizard, Gekko gekko. Twelve lizards were given single intraperitoneal injections of 12 mg/kg of mercuric chloride in saline. They were sacrificed after various intervals from 2 to 13 days. Four control animals received only saline injections.

Morphologic Changes of Rabbit Ovarian Follicles Following Copulation

1973 ◽  
Vol 31 ◽  
pp. 554-555
Author(s):  
David D. Cherney ◽  
Liberato J. A DiDio ◽  
Pietro Motta
Keyword(s):  
Electron Microscopy ◽  
Microscopic Study ◽  
Electron Micrographs ◽  
Electron Microscopic Study ◽  
Ovarian Follicles ◽  
Preovulatory Follicle ◽  
Electron Microscopic ◽  
Causal Factors ◽  
The Subject ◽  
Morphologic Changes

The interrelated processes enabling the ovum to be released from the mammalian ovary have been the subject of many investigations, which resulted in the advancement of several hypotheses; none, however, can as yet totally explain the morphophysiologic events leading to and responsible for ovulation.This investigation deals with a comprehensive light and electron microscopic study of the morphologic changes related to ovulation in the rabbit. Moreover, an attempt is made to establish a correlation between the modification of the follicle and perifollicular structures and their most likely causal factors.The morphology of the non-stimulated follicle is illustrated in Figure 1. Following copulation, follicles were removed at 2,4,6,8 and 10 hours, processed for electron microscopy, and comparable electron micrographs taken. As expected, the apex of the preovulatory follicle exhibited the most dramatic changes since it is the site where ovulation takes place.

Electron Microscopic Study of the Morphogenesis of Echovirus 23

1973 ◽  
Vol 31 ◽  
pp. 590-591
Author(s):  
C. M. Trant ◽  
R. M. Jamison
Keyword(s):  
Electron Microscopy ◽  
Microscopic Study ◽  
Electron Microscopic Study ◽  
Post Infection ◽  
Glass Surface ◽  
Kidney Cells ◽  
Electron Microscopic ◽  
Monolayer Cultures ◽  
Infected Cells ◽  
Infectious Cycle

The cytopathology which accompanies the replication of Echovirus 23 in monkey kidney cells has been studied. Cells grown in monolayer cultures in glass bottles were infected with Echovirus 23 and incubated at 37°C. At varying intervals, cells were harvested for electron microscopy. Multiple embeddings of specimens at each interval post-infection were performed to confirm and extend the observations. It was noted that as the interval postinfection lengthened, individual infected cells rounded and detached from the surface of the monolayer. As the infectious cycle continued, virtually all cells in the bottle became detached. Accordingly, each infected bottle provided two specimens for electron microscopy; a) those free in the supernatent. fluid and b) those remaining on.the glass surface.

scholarly journals Analytical electron microscopic study of mitochondrial inclusions in canine myocardial infarcts.

1976 ◽  
Vol 24 (3) ◽  
pp. 508-516 ◽  
Author(s):  
L M Buja ◽  
J H Dees ◽  
D F Harling ◽  
J T Willerson
Keyword(s):  
Electron Microscopy ◽  
Microscopic Study ◽  
Electron Microscopic Study ◽  
Mitochondrial Protein ◽  
Analytical Electron Microscopy ◽  
Muscle Cells ◽  
Electron Microscopic ◽  
Thin Sections ◽  
Mitochondrial Inclusions ◽  
Analytical Electron

An analytical electron microscopic study, utilizing scanning transmission electron microscopy and energy-dispersive x-ray spectroscopy, was made of two types of mitochondrial inclusions identified in canine myocardial infarcts. The data were obtained from thin sections of tissues that were fixed in aldehyde, osmicated and embedded in epoxy resin. Calcium peaks of variable intensity were detected in inclusions which contained very electron-dense spicular material and which were localized to muscle cells at the peripheries of the infarcts. These findings indicate that the spicular inclusions represent early stages in the process of mitochondrial calcification in myocardial infarcts. In contrast, calcium or other trace elements were not detected in moderately electron-dense amorphous inclusions which were present in mitochondria of muscle cells throughout the infarcts. With the tissue preparative techniques employed, the possibility cannot be excluded that the amorphous inclusions contained calcium, either in small amounts or in a readily diffusable state, in vivo. The data, however, are in accord with the previously advanced hypothesis that the amorphous inclusions represent precipitates of denatured mitochondrial protein formed during the evolution of irreversible cellular injury. This study provides further evidence that analytical electron microscopy can yield important information regarding the nature of various inclusions occurring in normal and diseased tissues.

An electron microscopic study of Amoeba proteus

1959 ◽  
Vol 150 (939) ◽  
pp. 216-232 ◽  
Keyword(s):  
Electron Microscopy ◽  
Fine Structure ◽  
Microscopic Study ◽  
Electron Microscopic Study ◽  
Amoeba Proteus ◽  
Contractile Vacuole ◽  
Electron Microscopic ◽  
Functional Implications ◽  
Food Vacuoles

Methods of fixing, embedding and sectioning of Amoeba proteus for electron microscopy are described. The fine structure of the various organelles: nucleus, mitochondria, food vacuoles; and the contractile vacuole, is discussed in detail. A number of minor unidentified objects has also been found. The functional implications of the structural findings are considered.

scholarly journals Canine Cutaneous Histiocytoma A Light and Electron Microscopic Study

1970 ◽  
Vol 7 (1) ◽  
pp. 12-27 ◽  
Author(s):  
D. F. Kelly
Keyword(s):  
Electron Microscopy ◽  
Endoplasmic Reticulum ◽  
Plasma Membrane ◽  
Acid Phosphatase ◽  
Microscopic Study ◽  
Mononuclear Cell ◽  
Electron Microscopic Study ◽  
Light And Electron Microscopy ◽  
Perinuclear Region ◽  
Electron Microscopic

Cutaneous histiocytomas from 4 dogs were examined by light and electron microscopy. A large (up to 10 μ in diameter) mononuclear cell with prominent filiform processes of the plasma membrane predominated. Its cytoplasm contained relatively small amounts of endoplasmic reticulum and mitochondria, only occasional lysosomes, fibrils, most obvious in the perinuclear region, and small amounts of cytoplasmic debris. Acid phosphatase was not detected. Fibroblasts and collagen formed a small part of the lesion, except at the junction with surrounding dermis, where fibers were plentiful. The morphologic features of the lesion are compatible with the suggestion that the predominant cell is of histiocytic type.

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