Serial sections and stereoscopy-complementary approaches to three-dimensional reconstruction

1986 ◽  
Vol 44 ◽  
pp. 36-37 ◽  
Author(s):  
John C. Russ ◽  
Thomas M. Hare
Keyword(s):  
Surface Area ◽  
Serial Section ◽  
Three Dimensional ◽  
Color Images ◽  
Physical Models ◽  
Serial Sections ◽  
Transparent Material ◽  
Modern Computer ◽  
Computer Equipment ◽  
Dimensional Reconstruction

Modelling of three-dimensional structures, either for purposes of geometrical measurement (eg. volume, surface area) or as an aid to visualization, has traditionally been carried out by a variety of different methods. Biologists, who are usually able to conveniently cut sections through their specimens, often make use of serial sections for this purpose. The most common interpretation of serial section photos has been by printing micrographs on transparent material, aligning them, and stacking them up. Occasionally, physical models of lucite, wood, clay or styrofoam have been constructed using the prints as templates, and with the advent of modern computer equipment, some digitization of the sections and their subsequent viewing or plotting with any viewpoint and orientation has enabled researchers to better see the structures represented. There has even been limited use of stereoscopy, that is, producing plots or on-screen color images of the feature outlines from two different viewpoints which can be visually merged to produce the illusion of relief.

THREE DIMENSIONAL RECONSTRUCTION OF QUADRIVALENTS AND MAPPING OF TRANSLOCATION BREAKPOINTS OF THE MOUSE TRANSLOCATIONS T(2;8) 26H AND T(9;17)138Ca

1980 ◽  
Vol 22 (2) ◽  
pp. 261-270 ◽  
Author(s):  
Anthony H. C. Choi
Keyword(s):  
Electron Micrographs ◽  
Serial Section ◽  
Three Dimensional ◽  
Serial Sections ◽  
Three Dimensional Reconstruction ◽  
Reciprocal Translocations ◽  
Mammalian Oocyte ◽  
Dimensional Reconstruction ◽  
Translocation Breakpoints ◽  
Serial Section Reconstruction

Three dimensional reconstruction from electron micrographs of serial sections reveals 18 synaptonemal complexes and a cross-shaped quadrivalent in the mouse pachytene oocytes of the heterozygous reciprocal translocations T(2;8)26H and T(9;17)138Ca. The unambiguous identification of translocation breakpoints on the quadrivalents has allowed the mapping of the translocation breakpoints on the chromosomes. The translocation breakpoints of T(2;8)26H are mapped at 73% and 45% from the telocentric centromeres of chromosomes 2 and 8, while those of T(9;17)138Ca are mapped at 41% and 45% from the telocentric centromeres of chromosomes 9 and 17 respectively. This report represents the first study of serial section reconstruction of a mammalian oocyte.

Three-Dimensional Reconstruction Using Stereo Pairs from Serial Thick Sections

1977 ◽  
Vol 35 ◽  
pp. 562-563
Author(s):  
Robert Glaeser ◽  
Thomas Bauer ◽  
David Grano
Keyword(s):  
Three Dimensional ◽  
Dimensional Structure ◽  
Serial Sections ◽  
Thin Sections ◽  
3 Dimensional ◽  
Transmission Electron ◽  
Freeze Dried ◽  
Dimensional Reconstruction ◽  
Stereo Imagery ◽  
Whole Mounts

In transmission electron microscopy, the 3-dimensional structure of an object is usually obtained in one of two ways. For objects which can be included in one specimen, as for example with elements included in freeze- dried whole mounts and examined with a high voltage microscope, stereo pairs can be obtained which exhibit the 3-D structure of the element. For objects which can not be included in one specimen, the 3-D shape is obtained by reconstruction from serial sections. However, without stereo imagery, only detail which remains constant within the thickness of the section can be used in the reconstruction; consequently, the choice is between a low resolution reconstruction using a few thick sections and a better resolution reconstruction using many thin sections, generally a tedious chore. This paper describes an approach to 3-D reconstruction which uses stereo images of serial thick sections to reconstruct an object including detail which changes within the depth of an individual thick section.

Cell morphology, volume, and surface area determinations from multilevel contouring within HVEM micrographs of serial sections and whole-cell mounts

1987 ◽  
Vol 45 ◽  
pp. 588-589
Author(s):  
M. Marko ◽  
A. Leith ◽  
D. Parsons
Keyword(s):  
Surface Area ◽  
Electron Micrographs ◽  
Cell Morphology ◽  
Individual Variation ◽  
Serial Section ◽  
Stereo Pair ◽  
Complex Structures ◽  
Serial Sections ◽  
Surface Areas ◽  
Computer Based

The use of serial sections and computer-based 3-D reconstruction techniques affords an opportunity not only to visualize the shape and distribution of the structures being studied, but also to determine their volumes and surface areas. Up until now, this has been done using serial ultrathin sections.The serial-section approach differs from the stereo logical methods of Weibel in that it is based on the Information from a set of single, complete cells (or organelles) rather than on a random 2-dimensional sampling of a population of cells. Because of this, it can more easily provide absolute values of volume and surface area, especially for highly-complex structures. It also allows study of individual variation among the cells, and study of structures which occur only infrequently.We have developed a system for 3-D reconstruction of objects from stereo-pair electron micrographs of thick specimens.

scholarly journals THE THREE-DIMENSIONAL RECONSTRUCTION OF THE XY CHROMOSOMAL PAIR IN HUMAN SPERMATOCYTES

1970 ◽  
Vol 45 (1) ◽  
pp. 43-53 ◽  
Author(s):  
A. J. Solari ◽  
Laura L. Tres
Keyword(s):  
Synaptonemal Complex ◽  
Three Dimensional ◽  
Strong Support ◽  
Serial Sections ◽  
Lateral Element ◽  
Spatial Reconstruction ◽  
Chromosomal Pair ◽  
Dimensional Reconstruction ◽  
Short Core ◽  
Apparent Association

The spatial reconstruction of the XY pair of chromosomes from human spermatocytes has been made by the study of serial sections 1000 A in thickness. The sex pair during zygotene-pachytene forms a condensed mass of chromatin that has two filamentous, electron-opaque cores: the long and the short core. During early pachytene both cores have a common ending region, about 0.4–0.8 µ long. This common end is a synaptonemal complex, and each of the cores forms a lateral element of that complex. The cores become more convoluted during middle pachytene forming "ringlike bodies." Nucleoli from spermatocytes have three distinct regions: (a) granular; (b) dense fibrillar; and (c) clear intermediate. Occasional association of the XY pair and the heteropycnotic "basal knobs" results in apparent association of nucleoli and the sex pair in a minority of cells. The evidence presented is interpreted as a strong support of the hypothesis of homologous regions in the human XY pair.

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