scholarly journals Genetic characterization ofMycobacterium aviumisolates recovered from humans and animals in Australia

1996 ◽  
Vol 116 (1) ◽  
pp. 41-49 ◽  
Author(s):  
M. M. Feizabadi ◽  
I. D. Robertson ◽  
D. V. Cousins ◽  
D. Dawson ◽  
W. Chew ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Mycobacterium Avium ◽  
Gel Electrophoresis ◽  
Genetic Characterization ◽  
Genetic Relationships ◽  
Pulsed Field Gel Electrophoresis ◽  
Pfge Type ◽  
Enzyme Electrophoresis ◽  
Pulsed Field ◽  
Multilocus Enzyme Electrophoresis

SummaryGenetic relationships amongst 115 mainly Australian isolates ofMycobacterium aviumwere assessed using multilocus enzyme electrophoresis (MEE). The isolates were divided into 58 electrophoretic types (ETs), with a mean genetic diversity of 0·29. Isolates from humans were closely related to but distinct from those cultured from birds, whilst some porcine isolates belonged to the same ETs as certain human isolates. Pulsed field gel electrophoresis (PFGE) was used to differentiate related isolates, and those from birds and some from other animals, including pigs, were distinguished from the human isolates. The results of MEE and PFGE suggested that certain strains ofM. aviummay be transmitted between birds and pigs, but there was no clear evidence of transmission to humans. The serovar of theM. aviumisolates was not obviously related to their ET assignment or their PFGE type.

Disparity between Multilocus Enzyme Electrophoresis, Microsatellite Markers and Pulsed-Field Gel Electrophoresis in epidemiological tracking of Candida albicans

2010 ◽  
Vol 82 (3) ◽  
pp. 265-281 ◽  
Author(s):  
Marcelo Fabiano Gomes Boriollo ◽  
Ricardo Antunes Dias ◽  
João Evangelista Fiorini ◽  
Nelma de Mello Silva Oliveira ◽  
Denise Madalena Palomari Spolidório ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Microsatellite Markers ◽  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Enzyme Electrophoresis ◽  
Pulsed Field ◽  
Multilocus Enzyme Electrophoresis

scholarly journals Staphylococcus aureus strains of type 95. Spread of a single clone

1994 ◽  
Vol 113 (3) ◽  
pp. 463-470 ◽  
Author(s):  
V. T. Rosdahl ◽  
W. Witte ◽  
M. Musser ◽  
J. O. Jarløv
Keyword(s):  
Staphylococcus Aureus ◽  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Enzyme Electrophoresis ◽  
Restriction Fragments ◽  
Pulsed Field ◽  
Multilocus Enzyme Electrophoresis ◽  
Single Clone ◽  
Clonal Origin ◽  
Reference Strains

SUMMARYStaphylococcus aureus strains of type 95 in Denmark have increased to a frequency of 20% of the total S. aureus population. A clonal origin and possible subdivision of these strains have been discussed. In the present investigation 35 epidemiologically unrelated S. aureus strains of type 95 as well as reference strains of other types have been analysed by other typing techniques including lectintyping, multilocus enzyme electrophoresis and pulsed-field gel electrophoresis of genomic restriction fragments. No subdivision could be achieved based on any of these methods and a clonal origin seems therefore possible.

scholarly journals Molecular Analysis of Mycobacterium avium Isolates by Using Pulsed-Field Gel Electrophoresis and PCR

1999 ◽  
Vol 37 (8) ◽  
pp. 2450-2455 ◽  
Author(s):  
Martine Pestel-Caron ◽  
Gabriel Graff ◽  
Gilles Berthelot ◽  
Jean-Louis Pons ◽  
Jean-François Lemeland
Keyword(s):  
Mycobacterium Avium ◽  
Gel Electrophoresis ◽  
Insertion Sequence ◽  
Genetic Relationships ◽  
Pcr Amplification ◽  
Pulsed Field Gel Electrophoresis ◽  
Pulsed Field ◽  
Copy Numbers ◽  
Region Ii ◽  
Laboratory Contamination

Genetic relationships among 46 isolates of Mycobacterium avium recovered from 37 patients in a 2,500-bed hospital from 1993 to 1998 were assessed by pulsed-field gel electrophoresis (PFGE) and PCR amplification of genomic sequences located between the repetitive elements IS1245 and IS1311. Each technique enabled the identification of 27 to 32 different patterns among the 46 isolates, confirming that the genetic heterogeneity ofM. avium strains is high in a given community. Furthermore, this retrospective analysis of sporadic isolates allowed us (i) to suggest the existence of two remanent strains in our region, (ii) to raise the question of the possibility of nosocomial acquisition ofM. avium strains, and (iii) to document laboratory contamination. The methods applied in the present study were found to be useful for the typing of M. avium isolates. In general, both methods yielded similar results for both related and unrelated isolates. However, the isolates in five of the six PCR clusters were distributed among two to three PFGE patterns, suggesting that this PCR-based method may have limitations for the analysis of strains with low insertion sequence copy numbers or for resolution of extended epidemiologic relationships.

scholarly journals Assessment of Resolution and Intercenter Reproducibility of Results of Genotyping Staphylococcus aureus by Pulsed-Field Gel Electrophoresis of SmaI Macrorestriction Fragments: a Multicenter Study

1998 ◽  
Vol 36 (6) ◽  
pp. 1653-1659 ◽  
Author(s):  
Alex van Belkum ◽  
Willem van Leeuwen ◽  
Mary Elizabeth Kaufmann ◽  
Barry Cookson ◽  
Françoise Forey ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Pfge Type ◽  
Data Sets ◽  
Bacterial Typing ◽  
Technical Problems ◽  
Pulsed Field ◽  
Pattern Similarity ◽  
Percent Similarity

Twenty well-characterized isolates of methicillin-resistantStaphylococcus aureus were used to study the optimal resolution and interlaboratory reproducibility of pulsed-field gel electrophoresis (PFGE) of DNA macrorestriction fragments. Five identical isolates (one PFGE type), 5 isolates that produced related PFGE subtypes, and 10 isolates with unique PFGE patterns were analyzed blindly in 12 different laboratories by in-house protocols. In several laboratories a standardized PFGE protocol with a commercial kit was applied successfully as well. Eight of the centers correctly identified the genetic homogeneity of the identical isolates by both the in-house and standard protocols. Four of 12 laboratories failed to produce interpretable data by the standardized protocol, due to technical problems (primarily plug preparation). With the five related isolates, five of eight participants identified the same subtype interrelationships with both in-house and standard protocols. However, two participants identified multiple strain types in this group or classified some of the isolates as unrelated isolates rather than as subtypes. The remaining laboratory failed to distinguish differences between some of the related isolates by utilizing both the in-house and standardized protocols. There were large differences in the relative genome lengths of the isolates as calculated on the basis of the gel pictures. By visual inspection, the numbers of restriction fragments and overall banding pattern similarity in the three groups of isolates showed interlaboratory concordance, but centralized computer analysis of data from four laboratories yielded percent similarity values of only 85% for the group of identical isolates. The differences between the data sets obtained with in-house and standardized protocols could be the experimental parameters which differed with respect to the brand of equipment used, imaging software, running time (20 to 48 h), and pulsing conditions. In conclusion, it appears that the standardization of PFGE depends on controlling a variety of experimental intricacies, as is the case with other bacterial typing procedures.

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